Yogita S. Ozarde, Vishnu P. Choudhari
Yogita S. Ozarde1, Vishnu P. Choudhari1,2
1Department of Pharmaceutical Chemistry, MAEER’s Maharashtra Institute of Pharmacy, Pune, India.
2Dr. Vishwanath Karad MIT World Peace University, School of Pharmacy, Pune, India.
Volume - 15,
Issue - 3,
Year - 2022
Euphorbia fusiformis Buch.-Ham.Ex D.Don, (Synonym: Euphorbia acaulis Roxb) is an infrequent medicinal, geophytic herb belonging to family Euphorbiaceae. The latex obtained from tubers of Euphorbia fusiformis was traditionally used as an antidote in scorpion and snake bites. Fresh latex has been externally applied to heal chronic wounds and cracks and to cure skin diseases. Previous research on the Euphorbia fusifornis primarily focused on phytochemical investigation and pharmacological screening of various solvent extracts of dried root powder. Considering the therapeutic potential of the latex, there is a need to explore the studies on plant latex. The present work deals with the determination of physicochemical properties, preliminary phytochemical analysis and acute oral toxicity study of latex of Euphorbia fusiformis. Physicochemical characterization and preliminary phytochemical studies were carried out by using ethanolic solution of latex. The studies revealed the presence of alkaloids, glycosides, terpenoids, steroids and saponins. Acute oral toxicity study of latex were carried out by acute toxic class method of OECD 423 guideline. Euphorbia fusiformis latex was found to be safe at the dose of 2000 mg/kg and therefore characterized as Category 5 in the GHS system. It is concluded that further studies are essential for isolation and identification of chemical constituents from Euphorbia fusiformis and screening of pharmacological activities to support the traditional claims.
Cite this article:
Yogita S. Ozarde, Vishnu P. Choudhari. Preliminary Qualitative Phytochemical Analysis and Acute Oral Toxicity Study of Latex of an Ethnomedicinal Plant Euphorbia fusiformis Buch.-Ham.Ex D.Don. Research Journal of Pharmacy and Technology. 2022; 15(3):1123-7. doi: 10.52711/0974-360X.2022.00188
Yogita S. Ozarde, Vishnu P. Choudhari. Preliminary Qualitative Phytochemical Analysis and Acute Oral Toxicity Study of Latex of an Ethnomedicinal Plant Euphorbia fusiformis Buch.-Ham.Ex D.Don. Research Journal of Pharmacy and Technology. 2022; 15(3):1123-7. doi: 10.52711/0974-360X.2022.00188 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2022-15-3-31
1. Juan H, Yan L, Chao-Jiang X, Shu-Xi J, Shi-Hong L, Sheng-Hong L. Chemical profile and defensive function of the latex of Euphorbia peplus. Phytochemistry. 2017; 136: 56-64.
2. Sake PK, Rajeswari B, Reddy VL, Damu AG, Sha Valli Khan PS. Isolation and Quantification of Flavonoid from Euphorbia antiquorum Latex and its Antibacterial Studies. Indian Journal of Advances in Chemical Science. 2013; 1(2): 117-122.
3. Manna S, Roy A. Euphorbia fusiformis and Co-Existed Plants: An Interspecific covariance analysis for experimental designing of its conservation. International Journal of Pure and Applied Biosciences. 2015; 3(2): 498-505.
4. Jagtap S, Satpute R. Phytochemical screening and antioxidant activity of tuber extracts of Euphorbia fusiformis var. Khandallensis (blatt. & hallb.) Binoj K. & N. P. Balakr. International Journal of Pharmaceutical Chemical and Biological Science. 2015; 5(3): 517-531.
5. Natarajan D, Britto SJ, Srinivasan K, Nagamurugan N, Mohanasundari C, Perumal G: Anti-bacterial activity of Euphorbia fusiformis-A rare medicinal herb. Journal of Ethnopharmacology. 2005; 102(1): 123-126.
6. Srinivas D, Reddy JK. Micropropagation of Euphorbia fusiformis Buch-Ham. A rare medicinal plant. Bioscience Discovery. 2017; 8(4): 766-770.
7. Manna S, Sur TK, Seal T, Roy A. Evaluation of galactagogue and antioxidant activities of the root extract of Euphorbia fusiformis. Asian Journal Traditional Medicine. 2013; 8(5): 20-29.
8. Kamalanathan D, Srinivasan R, Tamilselvan P, Yuvarajan R, Natarajan D. Antioxidant activities of leaf extracts of Euphorbia fusiformis Buch-Ham. ex D. Don (Euphorbiaceae). Free Radical and Antioxidants. 2015; 5(2): 83-89.
9. Bigoniya P, Shukla A, Singh CS. Dermal irritation and sensitization study of Euphorbia neriifolia latex and its anti-inflammatory efficacy. International Journal of phytomedicine. 2010; 2: 240-254.
10. Gohain HC, Sahu BP. Formulation and Evaluation of Mucoadhesive Tablet of Metformin HCl using Jack Fruit Latex (Artocarpus heterophyllus). Research Journal of Pharmacy and Technology. 2017; 10 (2): 371-377.
11. Marimuthu N, Viswanathan T, Mahendran R, Rathisre PR, Suganya J. Preliminary analysis of Phyto-constituents from the Leaf Extracts of Ballota nigra Linn. Research Journal of Pharmacy and Technology. 2017; 10(1): 161-165.
12. Kadali RM, Das MC, Bayya G, Kumar V. Preliminary Phytochemical Screening and Evaluation of effect of aqueous and Ethanolic leaf extracts of Chloroxylon swietenia on blood glucose in Streptozotocin Induced Diabetic rats. Research Journal of Pharmacy and Technology. 2019; 12(11): 5295-5300.
13. Mohamed ZF, Shanmugarajan TS, Somasundharam IJ, Ebens A, Neethu TT. Phytochemical investigation of Trichosanthes cucumerina linn for Analgesic Activity. Research Journal of Pharmacy and Technology. 2017; 10(1): 177-182.
14. Thilagavathi T, Kathiravan G. Phytochemical Analysis and Antimicrobial Activity of Ethonolic Leaf Extract of Ficus racemosa Linn. Research Journal of Pharmacy and Technology. 2017; 10(2): 537-540.
15. Khattak U, Ullah R, Khan SA, Ullah S, Saima. Pharmacognostic Evaluation and Analgesic Efficacy of Ethanolic Extract of Euphorbia dracunculoides L. Pharmacognosy Journal, 2017; 9(5): 644-653.
16. Pawar AT, Vyawahare NS. Antiurolithiatic activity of Abelmoschus moschatus seed extracts against zinc disc implantation-induced urolithiasis in rats. Journal of Basic and Clinical Pharmacy. 2016; 7(2): 32-38.
17. Vimal JB, Das SSM. Euphorbia antiquorum latex and its mosquitocidal potency against Aedes aegypti. Journal of Entomology and Zoology Studies. 2014; 2(6): 267-269.
18. Rindita, Hanani E, Saidah N. Pharmacognostical, Preliminary Phytochemical Evaluation and Flavonoids Content of Paederia foetida Linn. Research Journal of Pharmacognosy and Phytochemistry. 2019; 11(2): 81-86
19. Kane SR, Bhandari SV. In-vitro Phytochemical Investigation, Characterization & Antimicrobial Study of Latex of Euphorbia tirucalli Linn. International Journal of ChemTech Research. 2013; 5(5): 2344-2346.
20. Thorat SS., Patil PJ. Phytochemical Analysis and Pharmacological Screening of Caralluma fimfriata wall. Whole Plant Extract. Research Journal of. Pharmacognosy and Phytochemistry 2019; 11(4):217-221.
21. Upadhyaya C, Satish S, Hedge K. Screening of Antioxidant Property of Methanolic Extract of Euphorbia neriifolia Latex. International Journal of Pharmaceutical and Chemical Research. 2017; I(3): 541-545.
22. OECD 423 guidelines for the testing of chemicals. Acute Oral Toxicity – Acute Toxic Class Method- 2001: 1-14.
23. Jijith US, Sudhakaran Nair CR, Pramod K. Acute toxicity and Gross Behavioural effects of Alcoholic Extract of Anamirta cocculus willd. Research Journal Pharmacology and Pharmacodynamics. 2016; 8(1): 1-4.
24. Jain VK, Ahirwar D, Jain B, Ahirwar B. Evaluation of Acute Oral oxicity and Mast Cell Degranulation of an aqueous ethanolic extract of Tritium aestivum Linn. Research Journal of Pharmacy and Technology. 2018; 11(2): 643-648.
25. Anusuya N, Raju K, Manian S. Hepatoprotective and toxicological assessment of an ethnomedicinal plant Euphorbia fusiformis. Journal of Ethnopharmacology. 2010;127(2): 463-467.
26. Kavitha KS, Raju K. Pharmacological Studies on Euphorbia fusiformis Buch-Ham. ex. D. Don (Euphorbiaceae). Indian Journal of Natural Sciences. 2014; 4(22):1501-1508.
27. Selvan TA, Suresh R, Subramanian NS, Devi MR. Evaluation of Acute and Subacute Toxicity Studies of Polyherbal Extract on Rodents. Research Journal of Pharmacology and Pharmacodynamics. 2014: 6(2) :94-100.