Khoirul Anam, Bobi Prabowo, Meike Tiya Kusuma, Yuliati, Sri Winarsih, Tri Yudani Mardining Raras, Sumarno Reto Prawiro
Khoirul Anam1,2*, Bobi Prabowo3,4, Meike Tiya Kusuma3, Yuliati3, Sri Winarsih5, Tri Yudani Mardining Raras6, Sumarno Reto Prawiro5
1Doctoral Program in Medical Science, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia.
2Study Program of Medical Laboratory Technology, Institute of Health and Science Technology, Wiyata Husada Samarinda, Indonesia.
3Master Program in Biomedical Science, Faculty of Medicine, Universitas Brawijaya. Malang Indonesia.
4Department of Emergency Medicine, Dr. Iskak General Hospital, Tulungagung, Indonesia.
5Department of Clinical Microbiology, Faculty of Medicine, Universitas Brawijaya. Malang Indonesia.
6Department of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Brawijaya Malang. Indonesia.
Volume - 15,
Issue - 4,
Year - 2022
Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is the etiology of an outbreak Covid-19. SARS-CoV-2 has a structural part consisting of spike glycoprotein, nucleoprotein N, membrane M and envelopes small membrane pentamer E. Immunoinformatic approach epitope analysis is developed to identify both weak and robust epitopes. Our study aims to identify several epitopes present in the spike glycoprotein, envelope, and membrane protein from the SARCoV-2 surface, with the help of insilico approach that highly potential as vaccine candidates. Analysis of antigeninicity was performed with the Kolaskar and Tongaonkar Antigenicity software. Epitope Mapping was analyzed using Linear Epitope Prediction Bepired. The structure of proteins with epitope regions was visualized by software Pyrex and PyMOL. Conserve analysis was performed using bio edit software. HLA mimicry was analyzed through HLAPred software. Molecular docking between the epitope with HLA I and HLA II was validated by Chimera and PyMOL software. The toxicity test for candidate vaccine peptides was carried out using ToxinPred software. Our study found seven potential epitope candidates as vaccine candidates. The seven epitopes were derived from spike proteins (5 epitopes), envelope proteins (1 epitope), and membrane proteins (1 epitope). All epitope codes are conserved and are not the same as HLA in Humans. The docking test results show a value with low affinity so that a strong bond can provide a high immune response. Toxicity tests show that all epitopes are non-toxic and safe to use as vaccine ingredients. Seven peptides from the spike, envelope, membrane protein that showed potential as vaccine candidates against Covid-19.
Cite this article:
Khoirul Anam, Bobi Prabowo, Meike Tiya Kusuma, Yuliati, Sri Winarsih, Tri Yudani Mardining Raras, Sumarno Reto Prawiro. Multi Epitopes Potential on Surface SARS-CoV-2 Protein as a Covid-19 Vaccine Candidate. Research Journal of Pharmacy and Technology. 2022; 15(4):1437-2. doi: 10.52711/0974-360X.2022.00238
Khoirul Anam, Bobi Prabowo, Meike Tiya Kusuma, Yuliati, Sri Winarsih, Tri Yudani Mardining Raras, Sumarno Reto Prawiro. Multi Epitopes Potential on Surface SARS-CoV-2 Protein as a Covid-19 Vaccine Candidate. Research Journal of Pharmacy and Technology. 2022; 15(4):1437-2. doi: 10.52711/0974-360X.2022.00238 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2022-15-4-4
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