Hima Saila. M, Santhrani Thakur
Hima Saila. M1*, Santhrani Thakur2
1Assistant Professor, Department of Pharmacology, Sri Padmavathi School of Pharmacy, Tiruchanur, Tirupati, Chittoor Dist, Andhra Pradesh, India - 517503.
2Department of Pharmacology, Institute of Pharmaceutical Technology, Sri Padmavathi Mahila Visvavidyalayam, Tirupati, Chittoor Dist, Andhra Pradesh, India-517502.
Volume - 15,
Issue - 4,
Year - 2022
Aim and Objective: In this research study, transanethole at a dose of 250 and 500mg/kg p.o was investigated for its potency against Bilateral Common Carotid Artery occlusion (BCCAO) for 1 hr accompanied by 22 hrs reperfusion. Method: Healthy Albino Wistar rats (200–250gm) were divided randomly into 4 groups (n=9). Group I was considered as sham control, received 2% tween 80p.o, group II was considered as ischemic- reperfusion (disease control) and received 2% tween 80p.o and group III and IV were considered as prophylactic treatment groups and received transanethole at doses of 250mg/kg, p.o and 500mg/kg, p.o. respectively. After pretreatment with transanethole for14 days, rats were subjected to bilateral common carotid artery occlusion (BCCAO) for 1 hour accompanied by 22 hr reperfusion (I/R). After 22 hrs of reperfusion, motor coordination, behavioral assessment, infarct area, brain water content, excitatory mediators, inflammatory and apoptotic markers were determined. Results: Transanethole improved the motor coordination, lowered the brain water content, infarction volume and attenuated the levels of excitatory mediators. Western blotting analysis was performed to identify the proinflammatory mediators (TNF a, p38 MAPK), anti-inflammatory cytokine IL 10 and apoptotic marker proteins (Caspase 3, Bcl-2 and Bax) in brain tissue. Prophylactic treatment with trans anethole significantly down regulated the expressions of TNF-a, caspase 3, Bax, dephosphorylated p38 MAPK and upregulated the expressions of Bcl-2, IL 10 in a dose dependent manner in comparison with disease control group. Conclusion: An anti-inflammatory and anti- apoptotic activity of transanethole protected from cerebral ischemia reperfusion injury.
Cite this article:
Hima Saila. M, Santhrani Thakur. Transanethole protects against Global Cerebral Ischemia through its Anti-inflammatory and Anti apoptotic activity. Research Journal of Pharmacy and Technology. 2022; 15(4):1713-0. doi: 10.52711/0974-360X.2022.00287
Hima Saila. M, Santhrani Thakur. Transanethole protects against Global Cerebral Ischemia through its Anti-inflammatory and Anti apoptotic activity. Research Journal of Pharmacy and Technology. 2022; 15(4):1713-0. doi: 10.52711/0974-360X.2022.00287 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2022-15-4-53
1. Mozaffarian D, Benjamin EJ, Go AS, Arnett DK, Blaha MJ, Cushman M, de Ferranti S, Després JP, Fullerton HJ, Howard VJ et.al. American Heart Association Statistics Committee and Stroke Statistics Subcommittee. Heart disease and stroke statistics--2015 update: a report from the American Heart Association. Circulation. 131(4):e29-322. doi: 10.1161/CIR.0000000000000152.
2. George PM, Steinberg GK. Novel stroke therapeutics: unraveling stroke pathophysiology and its impact on clinical treatments. Neuron. 2015; 87(2): 297-309. doi: 10.1016/j.neuron.2015.05.041.
3. Freire RS, Morais SM, Catunda-Junior FE, Pinheiro DC. Synthesis and antioxidant, anti-inflammatory and gastroprotector activities of anethole and related compounds. Bioorg Med Chem. 2005;13(13):4353-4358. doi: 10.1016/j.bmc.2005.03.058.
4. Chouksey Divya, Preeti Sharma and Rajesh Singh Pawar. Biological activities and chemical constituents of Illicium verum hook fruits (Chinese star anise). Pelagia Research Library, Der Pharmacia Sinica. 2010; 1(3): 1-10. doi:10.1016/S1995-7645(13)60155-8
5. Ryu S, Seol GH, Park H, Choi IY. Trans-anethole protects cortical neuronal cells against oxygen–glucose deprivation/reoxygenation. Neurol Sci. 2014; 35(10):1541-1547. doi: 10.1007/s10072-014-1791-8.
6. Domiciano TP, de Oliveira Dalalio MM, Silva EL, Ritter AM, Estevão-Silva CF, Ramos FS, Caparroz-Assef SM, Cuman RK, Bersani-Amado CA. Inhibitory effect of anethole in nonimmune acute inflammation. Naunyn-Schmiedeberg's archives of pharmacology. 2013;Apr 1;386(4):331-338. : 10.1007/s00210-012-0820-5
7. Marinov V, Valcheva-Kuzmanova S. Review on the pharmacological activities of anethole. Scr Sci Pharm. 2015;2(2):14: doi.org/10.14748/ssp.v2i2.1141
8. Iwasaki Y Forebrain ischemia induced by temporary bilateral common carotid occlusion in normotensive rats. J of the neurolsci 1989; 90(2):155-165. 10.1016/0022-510x(89)90098-1. PMID: 2723680.
9. Gaur V, Aggarwal A, Kumar A. Protective effect of naringin against ischemic reperfusion cerebral injury: possible neurobehavioral, biochemical and cellular alterations in rat brain. Eur J of Pharmacol 2009; 616(1-3):147-154: 10.1016/j.ejphar.2009.06.056.
10. Reddy DS, Kulkarni SK. Possible role of nitric oxide in the nootropic and antiamnesic effects of neurosteroids on aging- and dizocilpine-induced learning impairment. Brain Res. 1989; 799(2):215-29:10.1016/s0006-8993(98)00419-3.
11. Hunter AJ, Hatcher J, Virley D, Nelson P, Irving E, Hadingham SJ, Parsons AA. Functional assessments in mice and rats after focal stroke. Neuropharmacol. 2000; 39(5):806-16: 10.1016/s0028-3908(99)00262-2
12. Willner P The validity of animal models of depression. Psychopharmacology. 1984; 83(1): 1-16: 10.1007/BF00427414.
13. Dheeraj Ahirwar, Bharti Ahirwar.Antidepressant effect of Nigella sativa in stress –induced depression. Research J. Pharm. And Tech.2020;13(4): 1611-1614. 10.5958/0974-360X.2020.00291.7
14. Joshi CN, Jain SK, Murthy PS. An optimized triphenyltetrazolium chloride method for identification of cerebral infarcts. Brain Res Protoc.2004; 13(1):11-17: 10.1016/j.brainresprot.2003.12.001
15. Yang Z, Weian C, Susu H, Hanmin W. Protective effects of mangiferin on cerebral ischemia-reperfusion injury and its mechanisms. Eur J Pharmacol. 2016;771:145-51. doi: 10.1016/j.ejphar.2015.12.003.
16. Tiwari M, Dwivedi UN, Kakkar P. Suppression of oxidative stress and pro-inflammatory mediators by Cymbopogoncitratus D. Stapf extract in lipopolysaccharide stimulated murine alveolar macrophages. Food ChemToxicol. 2010; 48(10):2913-2919. 10.1016/j.fct.2010.07.027
17. Bernt, Erich, and Hans Ulrich Bergmeyer. Glutathione." Methods of enzymatic analysis. Academic Press.194; 1643-1647.
18. Svoboda P, Mosinger B. Catecholamines and the brain microsomal Na, K-adenosinetriphosphatase--I. Protection against lipoperoxidative damage. BiochemPharmacol. 1981; 30(5):427-432. 10.1016/0006-2952(81)90626-2.
19. Wang Z, Liu T, Gan L, Wang T, Yuan X, Zhang B, Chen H, Zheng Q. Shikonin protects mouse brain against cerebral ischemia/reperfusion injury through its antioxidant activity. Eur J Pharmacol. 2010; 643(2-3):211-217: 10.1016/j.ejphar.2010.06.027
20. Rivera-Cervantes, MC, Castaneda-Arellano, R, Castro-Torres, RD, Gudino-Cabrera, G y Velasco, AIF, Camins, A & Beas-Zárate, C. P38 MAPK inhibition protects against glutamate neurotoxicity and modifies NMDA and AMPA receptor subunit expression. J of Mol Neuroscience. 2015; 55(3):596-608. 10.1007/s12031-014-0398-0.
21. Chaparro-Huerta V, Rivera-Cervantes MC, Flores-Soto ME, Gomez-Pinedo U and Beas-Zarate, C Proinflammatory cytokines and apoptosis following glutamate-induced excitotoxicity mediated by p38 MAPK in the hippocampus of neonatal rats. J of neuroimmunology. 2005; 165(1-2):53-62. 10.1016/j.jneuroim.2005.04.025
22. Bachstetter AD, Van Eldik LJ. The p38 MAP kinase family as regulators of proinflammatory cytokine production in degenerative diseases of the CNS. Aging and disease. 2010; 1(3):199. PMCID: PMC3377763.
23. Mori T, Wang X, Jung JC, Sumii T, Singhal AB, FiniME and Lo EH. Mitogen-activated protein kinase inhibition in traumatic brain injury: in vitro and in vivo effects. J of Cerebral Blood Flow &Metabolism.2002; 22(4):444-452. https://doi.org/10.1097%2F00004647-200204000-00008
24. Morganti JM, Goulding DS, Van Eldik LJ. Deletion of p38α MAPK in microglia blunts trauma-induced inflammatory responses in mice. J of neuroinflammation.2019; 16(1):98. https://doi.org/10.1186/s12974-019-1493-5.
25. Lobo-Silva, Carriche GM., Castro AG Balancing the immune response in the brain: IL-10 and its regulation. J of Neuroinflammation. 2019; 13:297. https://doi.org/10.1186/s12974-016-0763-8
26. Jiang G, Wu H, Hu Y, Li J, Li Q. Gastrodin inhibits glutamate-induced apoptosis of PC12 cells via inhibition of CaMKII/ASK-1/p38 MAPK/p53 signaling cascade. Cellular and Mol Neurobiology. 2014; 34(4):591-602. 10.1007/s10571-014-0043-z.