Sachin Kumar, Manoj Kumar Sharma, Manoj Kumar Sharma, Gautam Kumar
Sachin Kumar1*, Manoj Kumar Sharma1, Manoj Kumar Sharma2, Gautam Kumar3
1Department of Bioinformatics, JV College, Baraut (Baghpat), Uttar Pradesh, India.
2Department of Botany, JV College, Baraut (Baghpat), Uttar Pradesh, India.
3M. Tech. (Biotechnology), Meerut Institute of Engineering and Technology (MIET), Meerut, India.
Volume - 15,
Issue - 5,
Year - 2022
Polyhydroxyalkalonates (PHAs) have numerous industrial and medical applications, is being used in various medical applications such as scaffold, suture, heart valve and drug delivery etc. Poly 3-hydroxy butyrate (PHB) is the most common and important family member of PHAs. The bacterial strains which are able to produce higher quantities of PHA using low-cost substrates are always in demand. In the present study, a local strain of Bacillus sp. SM11 isolated from soil was screened for ability to produce biopolymer i.e., PHB (polyhydroxybutyrate). Various factors which have been found to have an impact on PHB production by the selected capable bacterial isolates were optimized viz., organic waste source (soya extract, whey, molasses, corn extract, and distillery waste liquor), nitrogen source (peptone, beef extract, yeast extract, ammonium chloride, and ammonium sulphate), pH, and trace elements. An optimized PHB yield of 3.53g/L was obtained using whey as a source of carbon, added with 1% of yeast extract as a nitrogen source at pH 6.0 in presence of calcium as a trace element. The outcome of the present study indicates that isolate is among one of the high PHB producing microorganism, using whey as a carbon source.
Cite this article:
Sachin Kumar, Manoj Kumar Sharma, Manoj Kumar Sharma, Gautam Kumar. Isolation of local strain of Bacillus sp. SM-11, producing PHB using different waste raw as substrate. Research Journal of Pharmacy and Technology. 2022; 15(5):2053-8. doi: 10.52711/0974-360X.2022.00339
Sachin Kumar, Manoj Kumar Sharma, Manoj Kumar Sharma, Gautam Kumar. Isolation of local strain of Bacillus sp. SM-11, producing PHB using different waste raw as substrate. Research Journal of Pharmacy and Technology. 2022; 15(5):2053-8. doi: 10.52711/0974-360X.2022.00339 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2022-15-5-21
1. Steinbuchel A. Polyhydroxyalkanoic acids. In Biomaterials: Novel Materials from Biological Sources, Edited by Byrom D. Macmillan Publishers, Basingstoke, United Kingdom. 1991; pp.123-213
2. Steinbuchel A, Valentin HE. Diversity of bacterial polyhydroxyalkanoic acids. FEMS Microbiology Letters 1995; 128: 219–228.
3. Singh P, Parmar N. Isolation and characterization of two novel polyhydroxybutyrate (PHB)-producing bacteria. African Journal of Biotechnology. 2011; 10 (24): 4907-4919.
4. Jackson DE, Srienc F. Novel methods to synthesize Polyhydroxyalkanoates. Biochemical Engineering Annals of the New York Academy of Sciences. 1994; 8 (745):134-148.
5. Khanna S, Srivastava AK. Recent advances in microbial polyhydroxyalkanoates. Process Biochemistry 2005; 40: 607–619.
6. Hasirci V, Kok FN. (2003). Polyhydroxybutyrate and Its Copolymers: Applications in the Medical Field. In Tissue Engineering and Novel Delivery Systems. Edited by Yaszemski MJ, Trantolo DJ, Lewandrowski K-U, Hasirci V, Altobelli DE and Wise DL Taylor & Francis, 2004; 1-19.
7. Brigham CJ, Sinskey AJ. Applications of Polyhydroxyalkanoates in the Medical Industry. International Journal of Biotechnology for Wellness Industries. 2012; 1: 53-60
8. Shrivastav A, Kim HY, Kim YR. Advances in the Applications of Polyhydroxyalkanoate Nanoparticles for Novel Drug Delivery System. BioMed Research International. 2013; Article ID 581684, A review. Available from: URL: http://dx.doi.org/10.1155/2013/581684
9. Luef KP, Stelzer F, Wiesbrock F. Poly(hydroxy alkanoate)s in Medical Applications. Chemical and Biochemical Engineering Quarterly 2012; 29(2): 287–297.
10. Ali I, Jamil N. Polyhydroxyalkanoates: Current applications in the medical field. Frontiers in Biology. 2016; 11: 19–27.
11. Balgun-Agbaje OA, Odeniyi OA, Odeniyi MA. Applications of Polyhydroxyalkanoates in Drug Delivery. Fabad Journal of Pharmaceutical Sciences. 2019; 44(2):147-158.
12. Bonartsev AP, Bonartseva GA, Reshetov IV, Kirpichnikov MP, Shaitan KV. Application of Polyhydroxyalkanoates in Medicine and the Biological Activity of Natural Poly(3-Hydroxybutyrate). Acta Naturae. 2019; 11(2):4-16.
13. Elmowafy E, Abdal-Hay A, Skouras A, Tiboni M, Casettari L, Guarino V. Polyhydroxyalkanoate (PHA): applications in drug delivery and tissue engineering, Expert Review of Medical Devices, 2019; 16 (6): 467-482.
14. Babos G, Rydz J, Kawalec M, Klim M, Fodor-Kardos A, Trif L, Feczko T. Poly(3-Hydroxybutyrate)-Based Nanoparticles for Sorafenib and Doxorubicin Anticancer Drug Delivery. International Journal of Molecular Science. 2020; 21(19): 7312
15. Pozo C, Toledo, MVM, Rodelas B, Lopez JG. Effects of culture conditions on the production of polyhydroxyalkanoates by Azotobacter chroococcum H23 in media containing a high concentration of alpechin (wastewater from olive oil mills) as primary carbon source. Journal of Biotechnology. 2002; 97:125-131.
16. Van-Thuoc D, Quillaguaman J, Mamo G, Mattiasson B (2008). Utilization of agricultural residues for poly(3-hydroxybutyrate) production by Halomonas boliviensis LC1. Journal of Applied Microbiology. 2008; 104(2): 420–428
17. Troschl C, Meixner K, Drosg B. Cyanobacterial PHA Production—Review of Recent Advances and a Summary of Three Years’ Working Experience Running a Pilot Plant. Bioengineering. 2017; 4 (2): 26.
18. Anon. Mannual of microbiological methods. Mc-Graw Hill Book Co., New York. 1957 (p157)
19. Juan ML, Gonzalez LW, Walker GC. A Novel Screening Method for Isolating Exopolysaccharide deficient Mutants. Applied and Environmental Microbiology. 1998; 64: 4600-4602.
20. Slepecky RA, Law JH. Assay of poly-βhydroxybutyric acid. Journal of Bacteriology. 1961; 82(1): 37-42.
21. Cappuccino TG, Sherman N. Microbiology, a laboratory manual. The Benjamin/cummings publishing, Company Inc., California. 1992
22. Krieg NR, Holt JG. Bergey’s Manual of Systematic Bacteriology, Williams and Wilkins, Baltimore, MD 1984.
23. Holt JG, Krieg NR, Sneath PHA, Stanley JT, Williams ST. Bergey’s manual of determinative bacteriology, 9th edn. Lippincot, Williams and Wilkins, Baltimore 2000.
24. Purushothama KV. Plastic a cancer in Nature: Trends, Problems and Policies in India. Research J. Humanities and Social Sciences 2015; 6(3): 209-212. doi: 10.5958/2321-5828.2015.00026.1
25. Antony A, George A, Jose J, Paul L, Babu MP, Sikha P S, Paul L, Sr. Naveena CMC, Joseph A. A Study to evaluate the effectiveness of Planned Teaching Programme on knowledge regarding the Ill effects of plastics on health and environment among housewives in selected community at Aluva. Int. J. Nur. Edu. and Research. 2018; 6(4):335-337. doi: 10.5958/2454-2660.2018.00081.9
26. Rani U. A Study to Assess the Effectiveness of Structured Programme on Knowledge regarding Hazards of Plastic usage among Housewives in selected Community Area, Bangalore. Int. J. of Advances in Nur. Management. 2019; 7(3):255-257. doi: 10.5958/2454-2652.2019.00058.1
27. Thiruchelvi. R, Kavitha K, Shankari K. New Biotechnological Routes for Greener Bio-plastics from Seaweeds. Research J. Pharm. and Tech 2020; 13(5):2488-2492. DOI: 10.5958/0974-360X.2020.00444.8
28. Saini RD. New Age Biodegradable and Compostable Plastics. Asian J. Research Chem. 2018; 11(1):179-188. doi: 10.5958/0974-4150.2018.00037.8
29. Thangavel S, Sujin David Rajan S., Kevin B.C. A Novel Approach to treat Bio Wastes Using Biodigesters and Microorganisms Employed to Increase Plastic Degradation. Research J. Engineering and Tech. Oct.-Dec., 2013; 4(4):221-225.
30. Yilmaz M, Beyatli Y. Poly-β-hydroxybutyrate (PHB) production by a Bacillus cereus MS strain in sugarbeet molasses. Sugar industry. 2005; 130(2):109-112.
31. Ataei SA, Vasheghani‐Farahani E, Shojaosadati SA, Tehrani HA. Isolation of PHA–Producing Bacteria from Date Syrup Waste. Macromolecular Symposia. 2008; 269 (1): 11-16.
32. Reddy AR, Kumar RB, Prabhakar KV. Isolation and Identification of PolyHydroxyButyrate (PHB) producing bacteria from Sewage sample. Research Journal of Pharmacy and Technology. 2017; 10(4): 1065-1069. DOI: 10.5958/0974-360X.2017.00193.7
33. Hire D, Pawar N, Kate S. Isolation and Screening of Poly β-Hydroxybutyrate Producing Microbes. International Journal of Research and Analytical Reviews. 2019; 6 (2):634-637.
34. Tehmina A, Shagufta S., Muhammed T, Huma M, Ali RA, Sehrish F, AbuSaeed H. Bioconversion of Agricultural Wastes to Polyhydroxybutyrate by Azotobacter vinelandii. Pakistan Journal of Zoology. 2020; 52 (6): 2227-2231.
35. Alagunachiyar M, Jeevitha RAJ, Handique M, S. Sudha Sri Kesavan. Isolation and Screening of Poly Hydroxy Butyrate (PHB) Producing Bacteria from Jeppiaar Salt Pane, Chennai. Research J. Pharm. and Tech. Sept, 2015; 8(9):1276-1280. doi: 10.5958/0974-360X.2015.00231.0
36. Jaiganesh R, Tattwaprakash C, Rahul KG, Iyappan S, Jaganathan MK. Characterization and Optimization of process parameter for maximum Poly (-β-) hydroxyl butyrate production by bacteria isolated from the soil. Research J. Pharm. and Tech. 2019; 12(10):4926-4930. doi: 10.5958/0974-360X.2019.00854.0
37. Deepa S, Bhuvanehswari P, Kanimozhi K, Panneerselvam A. Utilization of Industrial Waste for the Production of Poly- Beta- Hydroxy Butyrate (Phb) by Alcaligenes eutrophus. Research J. Science and Tech Oct.- Dec., 2013; 5(4):421-424.