Author(s): Gondo Mastutik, Nila Kurniasari, Alphania Rahniayu, Anny Setijo Rahaju, S. Eriaty N. Ruslan, Khafidhotul Ilmiah, Bagus Setyoboedi, Erna Sulistyani

Email(s): gondomastutik@fk.unair.ac.id , gondomastutik@gmail.com

DOI: 10.52711/0974-360X.2022.00357   

Address: Gondo Mastutik1*, Nila Kurniasari1,2, Alphania Rahniayu1,2, Anny Setijo Rahaju1,2, S. Eriaty N. Ruslan3, Khafidhotul Ilmiah1, Bagus Setyoboedi4,5, Erna Sulistyani6
1Department of Anatomic Pathology, Faculty of Medicine, Universitas Airlangga, Surabaya - 60132, Indonesia.
2Department of Anatomic Pathology, Dr. Soetomo General Academic Hospital, Surabaya - 60132, Indonesia.
3Institute of Tropical Diseases, Universitas Airlangga, Surabaya - 60132, Indonesia.
4Department of Child Health of Pediatric, Faculty of Medicine, Universitas Airlangga, Surabaya - 60132, Indonesia.
5Department of Child Health of Pediatric, Dr. Soetomo General Academic Hospital, Surabaya - 60132, Indonesia.
6Department of Oral Medicine, Faculty Dentistry, Jember University, Jember- 68121, Indonesia.
*Corresponding Author

Published In:   Volume - 15,      Issue - 5,     Year - 2022


ABSTRACT:
Cholestatic infants are associated with congenital abnormalities or viral infections, such as cytomegalovirus (CMV) infection. CMV can be detected by polymerase chain reaction (PCR) in body fluids, including urine which can be obtained easily and is non-invasive. The objective was to detect CMV in urine specimens of cholestasis infants and to analyze its correlation with serological status. This was a descriptive observational study with the cross-sectional approach, used urine from 39 cholestatic infants who meet the inclusion and exclusion criteria and have been approved by Ethics Committee. The nested-PCR was performed from extracted urine and unextracted direct urine. Serological data of immunoglobulin (Ig) M and IgG data were collected. Data were analyzed by Chi-square. Detection of CMV from extracted urine by PCR showed positive in 87.2% patients and from unextracted urine was positive in 48.7% patients. Serological status showed that IgM was positive in 41.0% patients and IgG was positive in 89.7% patients. The acute infection (IgM+ IgG+) was found in 41.0% patients, past infection (IgM-IgG+) was 48.7% patients, and not infected (IgM-IgG-) was in 10.3% patients. The acute infection (IgM+ IgG+), past infection (IgM-IgG+) and not infected (IgM-IgG-) was found in 41.0%, 48.7%, and 10.3% patients, respectively. The correlation between PCR CMV from extracted urine with serological CMV was moderate, while the unextracted urine was low. It indicates that to detect the infection of CMV, PCR technique is more accurate than serological testing, and the extracted urine is more appropriate specimen as PCR template than direct urine.


Cite this article:
Gondo Mastutik, Nila Kurniasari, Alphania Rahniayu, Anny Setijo Rahaju, S. Eriaty N. Ruslan, Khafidhotul Ilmiah, Bagus Setyoboedi, Erna Sulistyani. Detection of Cytomegalovirus in Urine Specimen of Cholestatic Infants by Polymerase Chain Reaction. Research Journal of Pharmacy and Technology. 2022; 15(5):2151-7. doi: 10.52711/0974-360X.2022.00357

Cite(Electronic):
Gondo Mastutik, Nila Kurniasari, Alphania Rahniayu, Anny Setijo Rahaju, S. Eriaty N. Ruslan, Khafidhotul Ilmiah, Bagus Setyoboedi, Erna Sulistyani. Detection of Cytomegalovirus in Urine Specimen of Cholestatic Infants by Polymerase Chain Reaction. Research Journal of Pharmacy and Technology. 2022; 15(5):2151-7. doi: 10.52711/0974-360X.2022.00357   Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2022-15-5-39


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