Author(s):
Sathish M, Nandhini V, Suresh R
Email(s):
sathishpharmacy14@gmail.com
DOI:
10.52711/0974-360X.2022.00416 
Address:
Sathish M1*, Nandhini V1, Suresh R2
1Department of Pharmaceutical Chemistry, College of Pharmacy, Madras Medical College, Chennai, Tamil Nadu, India.
2Greensmed Labs, Thoraipakkam, Chennai, Tamil Nadu, India.
*Corresponding Author
Published In:
Volume - 15,
Issue - 6,
Year - 2022
ABSTRACT:
Objective: To investigate the in vitro antidiabetic effect on the leaf extracts of Jatropha glandulifera Roxb. Methods: The Preliminary phytochemical screening was investigated on extracts using standard procedure. The in vitro a-amylase and a-glucosidase inhibition assay was carried out at different concentration such as 10, 20, 40, 80, 160, and 320µg/ml of extracts. The acarbose and voglibose were used as standards respectively. The absorbance was recorded by spectrophotometer. The percentage inhibition and IC50 value were calculated for both the assays. Results: The ethyl acetate extract of Jatropha glandulifera Roxb has shown good inhibition against a-amylase and a-glucosidase with an IC50 value of 21.6137 and 25.8257µg/ml respectively. The phytochemical investigation revealed that the ethyl acetate extract have various phytochemical constituents compared to other extracts like alkaloids, carbohydrates, saponins, Flavonoids and glycosides. These phytoconstitients may be responsible for observed anti diabetic activity. Conclusion: This study concludes that the ethyl acetate extract of Jatropha glandulifera Roxb exhibits better inhibitory activity against both a-amylase and a-glucosidase.
Cite this article:
Sathish M, Nandhini V, Suresh R. In vitro Alpha amylase and Alpha glucosidase enzyme inhibition of leaf extracts of Jatropha glandulifera Roxb. Research Journal of Pharmacy and Technology. 2022; 15(6):2493-7. doi: 10.52711/0974-360X.2022.00416
Cite(Electronic):
Sathish M, Nandhini V, Suresh R. In vitro Alpha amylase and Alpha glucosidase enzyme inhibition of leaf extracts of Jatropha glandulifera Roxb. Research Journal of Pharmacy and Technology. 2022; 15(6):2493-7. doi: 10.52711/0974-360X.2022.00416 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2022-15-6-20
REFERENCES:
1. Ahmed Am. History of Diabetes mellitus. Saudi Med J. 2002 Apr;23 (4):373-8. PMID: 11953758
2. Classification of diabetes mellitus 2019, World Health Organization. ISBN 978-92- 4-151570-2.
3. International Diabetes Federation. IDF Diabetes Atlas, 9th edition. Brussels, Belgium: 2019. https://www.diabetesatlas.org.
4. Sharma N, Sharma M, Bindal MC. Potential antidiabetic herbal drugs: A comparative review of marketed products. Research Journal of Pharmacognosy and Phytochemistry. 2010 Apr 24;2(2):115-21.
5. Ravichandiran V, Nirmala S. A systematic Review on Antidiabetic medicinal Plants. Research Journal of Pharmacognosy and Phytochemistry. 2013 May 1;5(3):155-68.
6. Perez G RM, Zavala S MA, Perez G S, Perez G C. Antidiabetic effect of compounds isolated from plants. Phytomedicine. 1998 Mar;5(1):55-75. doi.org/10.1016/S0944-7113(98)80060-3
7. Ayurvedic Pharmacopoeia of India (API) Volume 5. Page no: 26.
8. Ballantine JA. The isolation of two esters of the naphtha quinone alcohol, shikonin, from the shrub Jatropha glandulifera. Phytochemistry. 1969 Aug 1;8(8):1587-90. doi.org/10.1016/S0031-9422(00)85935-X
9. Parthasarathy MR, Saradhi KP. A coumarino-lignan from Jatropha glandulifera. Phytochemistry. 1984;23(4):867-9. doi.org/10.1016/S0031-9422(00)85045-1
10. Bhagat RB, Kulkarni DK. Phytochemical evaluation and In vitro Antimicrobial activity of Jatropha glandulifera Roxb.(Family Euphorbiaceae). International Journal of Pharmacy and Life Sciences. 2013 Dec 1;4(12).
11. Viswanathan MB, Ananthi JD, Venkatesan N. Review on Jatropha (2018).
12. https://sites.google.com/site/efloraofindia/species/a---l/e/euphorbiaceae/jatropha/jatropha-glandulifera
13. Nikhat F, Satyanarayana D, Shastri CS, Rajni S, Sheikh AS. The Phytochemicals explored from the roots of Syzygium cuminni (L) skeel assessed for Anti-hyperglycemic activity. Asian Journal of Research in Chemistry. 2013 Oct 28;6(10):920-5.
14. Harborne J B. Phytochemical methods 11th edition. New York: In Chapman & Hall; 1984;4-5.
15. Kokate C K. Practical Pharmacognosy. Preliminary Phytochemical Screening. New Delhi: Vallabh prakashan; Chapter 6, 106 -111.
16. Jancy VJ, Kalaichelvan V, Balakrishnan N. Phytochemical Analysis and Anti-Oxidant Activity of various Extracts of Plant Cassia auriculata. Research Journal of Pharmacy and Technology. 2020 Dec 18;13(12):6150-5. doi.org/10.5958/0974-360X.2020.01073.2
17. Agarwal A, Gupta V, Gupta A, Jayalakshmi S. Phytochemical Evaluation of Extracts of Stem of Eclipta alba (Bhringaraja). Research Journal of Pharmacology and Pharmacodynamics. 2009;1(3):134-9.
18. Jose BE, Panneerselvam P. In Vitro Antidiabetic activity of Ethanol and Aqueous leaf extracts of Azima tetracantha Lam. Research Journal of Science and Technology. 2019 Mar 31;11(1):64-8. doi.org/10.5958/2349-2988.2019.00009.3
19. Khamis M, Talib F, Rosli NS, Dharmaraj S, Mohd KS, Srenivasan S, Latif ZA, Utharkar MR. In vitro α-amylase and α-glucosidase inhibition and increased glucose uptake of Morinda citrifolia fruit and scopoletin. Res J Pharm Technol. 2015 Feb 28;8:189-93.
20. In vitro alpha-amylase, alpha-glucosidase, lipase inhibitory and cytotoxic activities of tuber extracts of Kedrostis africana (L.) Cogn. doi.org/10.1016/j.heliyon.2018.e00810.
21. Apostolidis E, Kwon YI, Shetty K. Innovative Food Science and Emerging Technologies 2007; 8:46 - 54. doi.org/10.1016/j.ifset.2006.06.001
22. Anjitha R, Subbaraya U, Narasimhan G. Integrated Invitro-Insilico screening strategy of Ethanolic extract of leaves of Passiflora edulis Sims var. flavicarpa against Alpha Glucosidase. Research Journal of Pharmacy and Technology. 2020 Mar 1;13(3):1309-13. doi.org/10.5958/0974-360X.2020.00241.3
23. El-Saber Batiha G, Magdy Beshbishy A, G Wasef L, Elewa YH, A Al-Sagan A, ElHack A, Mohamed E, Taha AE, M Abd-Elhakim Y, Prasad Devkota H. Chemical constituents and pharmacological activities of garlic (Allium sativum L.): A review. Nutrients. 2020 Mar;12(3):872. doi.org/10.3390/nu12030872
24. Sathish M. In vitro and in vivo antidiabetic activity on leaves of Merremia hederacea (Burm. f.) Hallier f. International Journal of Green Pharmacy (IJGP). 2019 Dec 18;13(04). doi.org/10.22377/ijgp.v13i04.2750
25. Formagio, A.S.N.; Volobuff, C.R.F.; Santiago, M.; Cardoso, C.A.L.; Vieira, M.D.C.; Pereira, Z.V. Evaluation of Antioxidant Activity, Total Flavonoids, Tannins and Phenolic Compounds in Psychotria Leaf Extracts. Antioxidants 2014, 3, 745–757. doi.org/10.3390/antiox3040745
26. Raju SN, Sathis Kumar D, David Banji OB, Yogeswaran P, Narender Prasad D, Harani A. Inhibitory effects of ethanolic extract of physalis minima on amylase, Lipase and alpha glucosidase. Research Journal of Pharmacognosy and Phytochemistry. 2010 Apr 24;2(2):159-62.
27. Helen PM, Bency BJ. Inhibitory potential of Amaranthus viridis on α-amylase and glucose entrapment efficacy In vitro. Research Journal of Pharmacy and Technology. 2019 May 31;12(5):2089-92. doi.org/10.5958/0974-360X.2019.00346.9