ABSTRACT:
A stability indicating UV spectrophotometric method has been developed and validated for the estimation of voglibose in pharmaceutical preperation. The proposed method utilized 0.1 N NaOH as solvent and detection was carried out at 214.5nm. Experiments were designed for determining linearity, limit of detection and quantitation, accuracy, precision and specificity of this analytical method. The stability studies of voglibose was carried out under acid, alkaline, thermal, oxidative and photolytic. The responses were linear in the concentration range of 5-25µg/ml with correlation coefficient of 0.9997. The % RSD for both inter-day and intra-day precision were less than 2%. The recovery of drug from sample was found to be 99.6-100.4%. Although the degradation products of stress condition had not been identified, the method had been able to detect the changes due to stress condition. The stated method can be used as stability indicating method with high degree of linearity, accuracy and precision for assay of voglibose in routine pharmaceutical analysis of tablet formulation. The method was found to be simple and cost effective. Hence this method can be successfully used to study stress degradation behavior of voglibose in small industry where high end instruments are not available.