Author(s):
Thi Dac Ngan Thai, Nguyen Tuong Vi Le, Van Chinh Nguyen, Tong Hung Quach, Cuu Khoa Nguyen
Email(s):
nckhoavnn@yahoo.com
DOI:
10.52711/0974-360X.2022.00459
Address:
Thi Dac Ngan Thai1,2, Nguyen Tuong Vi Le1,2, Van Chinh Nguyen1, Tong Hung Quach1, 2, Cuu Khoa Nguyen1,2*
1Department of Materials and Pharmaceutical Chemistry, Institute of Applied Materials Science, Vietnam Academy of Science and Technology, 01 TL29 District 12, Ho Chi Minh City 700000, Vietnam.
2Graduate University of Science and Technology, Vietnam Academy of Science and Technology, Hanoi 100000, Vietnam.
*Corresponding Author
Published In:
Volume - 15,
Issue - 6,
Year - 2022
ABSTRACT:
Active ingredient (AI), particularly quercetin (Q), has been known as types of nature-derived chemotherapic agents in cancer treatment. However, the advantages of this agent concerning antineoplastic activity were restricted by its poor water solubility. Therefore, the encapsulation of AI in nano-mediated drug delivery is expected to create diverse effects and can sufficiently increase their therapeutic outcomes. The aim of this present study was to effectively prepare folate-conjugated liposome (L-F) that can enhance the delivery of Q. L-F containing Q (Q-L-F) was successfully prepared by thin film technique, using tween 80-ethylenediamine-acid folic as the surface-modified moiety. Physicochemical parameters, including morphology, particles size, zeta potential, drug encapsulation efficiency and release profiles were investigated. In addition, in vitro cytotoxicity of the prepared formulation was evaluated against NCI-H460 cell line. Results showed that the prepared Q-L-F had a mean size of about 166.8 nm with low polydispersity index (below 0.5) and high encapsulation efficiency (96.6%). The release profile showed a sustained release of Q up to 48 h. Moreover, Q-L-F liposomal system was proposed to have the enhanced toxicity effect toward cancerous cells with expressed folate receptors due to the targeting of folic acid conjugated. In support for this, cell proliferation using SRB assay on NCI-H460 cells demonstrated that Q-L-F exhibited higher cytotoxicity than quercetin loaded conventional liposome (Q-L). For the purpose of researching, the data could serve as proof for the potential of L-F as a sustained delivery system for Q in anti-cancer therapy.
Cite this article:
Thi Dac Ngan Thai, Nguyen Tuong Vi Le, Van Chinh Nguyen, Tong Hung Quach, Cuu Khoa Nguyen. Folate-conjugated liposome as effective Drug delivery system for Quercetin. Research Journal of Pharmacy and Technology. 2022; 15(6):2741-6. doi: 10.52711/0974-360X.2022.00459
Cite(Electronic):
Thi Dac Ngan Thai, Nguyen Tuong Vi Le, Van Chinh Nguyen, Tong Hung Quach, Cuu Khoa Nguyen. Folate-conjugated liposome as effective Drug delivery system for Quercetin. Research Journal of Pharmacy and Technology. 2022; 15(6):2741-6. doi: 10.52711/0974-360X.2022.00459 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2022-15-6-63
REFERENCES:
1. Bachmetov, L, Gal‐Tanamy, M, Shapira, A, et al. Suppression of hepatitis C virus by the flavonoid quercetin is mediated by inhibition of NS3 protease activity. Journal of viral hepatitis. 2012 Aug 16; 19(2):e81-88.doi: 10.1111/j.1365-2893.2011.01507.x
2. Thapa, M, Kim, Y, Desper, J, et al. Synthesis and antiviral activity of substituted quercetins. Bioorganic & medicinal chemistry letters. 2012 Jan 1; 22(1):353-356.doi: 10.1016/j.bmcl.2011.10.119
3. Mohanty, S, Pal, A, Si, SC. Flavonoid as Nutraceuticals: A Therapeutic approach to Rheumatoid Arthritis. Research Journal of Pharmacy and Technology. 2020 Apr 9; 13(2):991-998.doi: 10.5958/0974-360X.2020.00184.5
4. Saha, S, Roy, A, Bahadur, S, et al. Fabrication and in-vitro evaluation of liposomal quercetin and its optimization. Research Journal of Pharmacy and Technology. 2018 Jun 12; 11(1):61-64.doi: 10.5958/0974-360X.2018.00012.4
5. Choubey, A, Bajpai, P, Jain, S. Nanomedicines based Drug delivery system and their significant role in Herbal Formulations: A Review. Research Journal of Pharmacy and Technology. 2020 Oct 28; 13(10):5034-5039.doi: 10.5958/0974-360X.2020.00881.1
6. Patra, M, Mukherjee, R, Banik, M, et al. Calcium phosphate-quercetin nanocomposite (CPQN): A multi-functional nanoparticle having pH indicating, highly fluorescent and anti-oxidant properties. Colloids and Surfaces B: Biointerfaces. 2017 Jun 1; 154:63-73.doi: 10.1016/j.colsurfb.2017.03.018
7. Le, NTT, Nguyen, DTD, Nguyen, NH, et al. Methoxy polyethylene glycol–cholesterol modified soy lecithin liposomes for poorly water‐soluble anticancer drug delivery. Journal of Applied Polymer Science. 2021 Sep 22; 138(7):49858.doi: 10.1002/app.49858
8. Le, NTT, Vu, MT, Nguyen, NH, et al. Preparation and in vitro evaluation of PEGylated liposomes as effective nanocarrier for delivery of oxaliplatin. Journal of Materials Research. 2021 Jan 14; 36:1-12.doi: 10.1557/s43578-021-00105-9
9. Dai Hai, N. 5-fluorouracil encapsulated CS-mPEG nanogels for controlling drug release. Vietnam Journal of Chemistry. 2017 Aug 31; 55(4):446.doi: 10.15625/2525-2321.2017-00489
10. Hamzah, H, Hertiani, T, Pratiwi, SUT, et al. Efficacy of Quercetin against Polymicrobial Biofilm on Catheters. Research Journal of Pharmacy and Technology. 2020 13(11):5277-5282.doi: 10.5958/0974-360X.2020.00923.3
11. Chauhan, SB, Gupta, V. Recent advances in liposome. Research Journal of Pharmacy and Technology. 2020 Apr 30; 13(4):2053-2058.doi: 10.5958/0974-360X.2020.00369.8
12. Yadav, C, Asif, M, Akhtar, J, et al. An outline on Curcumin: Its pro-liposome and Liposome Formulations. Research Journal of Pharmacy and Technology. 2020 Dec; 13(12):6276-6283.doi: 10.5958/0974-360X.2020.01092.6
13. Scaranti, M, Cojocaru, E, Banerjee, S, et al. Exploiting the folate receptor α in oncology. Nature Reviews Clinical Oncology. 2020 Jun; 17(6):349-359.doi: 10.1038/s41571-020-0339-5
14. Rana, A, Bhatnagar, S. Advancements in folate receptor targeting for anti-cancer therapy: A small molecule-drug conjugate approach. Bioorganic Chemistry. 2021 Jul; 112:104946-104946.doi: 10.1016/j.bioorg.2021.104946
15. Ruozi, B, Tosi, G, Forni, F, et al. Ketorolac tromethamine liposomes: encapsulation and release studies. Journal of liposome research. 2005 Dec 9; 15(3-4):175-185.doi: 10.1080/08982100500364214
16. Liang, X, Fan, J, Zhao, Y, et al. A targeted drug delivery system based on folic acid-functionalized upconversion luminescent nanoparticles. Journal of biomaterials applications. 2017 Mar 28; 31(9):1247-1256.doi: 10.1177/0885328217701289
17. Nguyen, M-NT, Ho-Huynh, T-D. Selective cytotoxicity of a Vietnamese traditional formula, Nam Dia long, against MCF-7 cells by synergistic effects. BMC complementary and alternative medicine. 2016 Jul 16; 16(1):1-10.doi: 10.1186/s12906-016-1212-z
18. Kumar, D, Sakhare, N, Das, S, et al. Development of technetium-99m labeled ultrafine gold nanobioconjugates for targeted imaging of folate receptor positive cancers. Nuclear Medicine Biology. 2021 Feb; 93:1-10.doi: 10.1016/j.nucmedbio.2020.11.001
19. Mutalik, S, Salian, SR, Avadhani, K, et al. Liposome encapsulated soy lecithin and cholesterol can efficiently replace chicken egg yolk in human semen cryopreservation medium. Systems biology in reproductive medicine. 2014 Jun; 60(3):183-188.doi: 10.3109/19396368.2014.902521
20. Yokota, D, Moraes, M, Pinho, S. Characterization of lyophilized liposomes produced with non-purified soy lecithin: a case study of casein hydrolysate microencapsulation. Brazilian Journal of Chemical Engineering. 2012 Jun; 29(2):325-335.doi: 10.1590/S0104-66322012000200013
21. Zhang, W. Nanoparticle aggregation: principles and modeling. Nanomaterial. 2014 811:19-43.doi: 10.1007/978-94-017-8739-0_2
22. Kumar, PR, Lakshmi, AV. An overview on nanobased drug delivery system. Research Journal of Pharmacy and Technology. 2020 Oct; 13(10):4996-5003.doi: 10.5958/0974-360X.2020.00875.6
23. Thomas, JA, Abraham, S, Raju, SP, et al. Formulation and Evaluation of Anti-Arthritis Drug Loaded Stealth Liposomes. Research Journal of Pharmacy and Technology. 2021 14(3):1313-1318.doi: 10.5958/0974-360X.2021.00233.X
24. Joseph, AE, Abraham, S, Raju, SP, et al. Formulation and Evaluation of levofloxacin stealth liposome using different polymers. Research Journal of Pharmacy and Technology. 2021 14(3):1493-1498.doi: 10.5958/0974-360X.2021.00265.1
25. Le, NTT, Nguyen, CK, Nguyen, DH. Carboplatin delivery system based on poly (ethylene glycol) methyl ether–cholesterol modified soy lecithin liposomes. Advances in Natural Sciences: Nanoscience and Nanotechnology. 2020 Nov 30; 11(4):045016.doi: 10.1088/2043-6254/abcaf7
26. Gao, W. Preparation and evaluation of folate receptor mediated targeting liposomes. Liposome-Based Drug Delivery Systems. 2021 Jul 1; doi: 10.1007/978-3-662-49231-4_12-1:167-178.doi: 10.1007/978-3-662-49231-4_12-1
27. Li, Z, Xiong, X, Peng, S, et al. Novel folated pluronic F127 modified liposomes for delivery of curcumin: preparation, release, and cytotoxicity. Journal of microencapsulation. 2020 Feb 17; 37(3):220-229.doi: 10.1080/02652048.2020.1720030
28. Nikam, NR, Patil, PR, Vakhariya, R, et al. Liposomes: A Novel Drug Delivery System: An Overview. Asian Journal of Pharmaceutical Research. 2020 Jun 3; 10(1):23-28.doi: 10.5958/2231-5691.2020.00005.2
29. Mulla, TS, Thorat, MS, Rayate, Y, et al. Liposome as a drug carrier. Asian Journal of Research in Pharmaceutical Science. 2019 Jul 3; 9(2):141-147.doi: 10.5958/2231-5659.2019.00021.3
30. Gang, W, Jie, WJ, Ping, ZL, et al. Liposomal quercetin: evaluating drug delivery in vitro and biodistribution in vivo. Expert opinion on drug delivery. 2012 Jun; 9(6):599-613.doi: 10.1517/17425247.2012.679926
31. Gazzano, E, Rolando, B, Chegaev, K, et al. Folate-targeted liposomal nitrooxy-doxorubicin: An effective tool against P-glycoprotein-positive and folate receptor-positive tumors. Journal of controlled release. 2018 Jan 28; 270:37-52.doi: 10.1016/j.jconrel.2017.11.042
32. Karpuz, M, Silindir-Gunay, M, Kursunel, MA, et al. Design and in vitro evaluation of folate-targeted, co-drug encapsulated theranostic liposomes for non-small cell lung cancer. Journal of Drug Delivery Science Technology. 2020 Jun; 57:101707.doi: 10.1016/j.jddst.2020.101707
33. Tan, S, Wang, G. Lung cancer targeted therapy: folate and transferrin dual targeted, glutathione responsive nanocarriers for the delivery of cisplatin. Biomedicine Pharmacotherapy. 2018 Jun; 102:55-63.doi: 10.1016/j.biopha.2018.03.046