Author(s):
Retno Budiarti, Ediono, Ni Made Mertaniasih, Soedarsono
Email(s):
nmademertaniasih@gmail.com
DOI:
10.52711/0974-360X.2022.00498 
Address:
Retno Budiarti1, Ediono2, Ni Made Mertaniasih3*, Soedarsono4
1Microbiology Department, Medical Faculty Hang Tuah University, Surabaya, Indonesia.
2Pulmonology Department, Dr Ramelan Hospital, Surabaya, Indonesia.
3Medical Microbiology Department, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia.
4Department of Pulmonology and Medical Respirology, Faculty of Medicine Universitas Airlangga, Surabaya, Indonesia.
*Corresponding Author
Published In:
Volume - 15,
Issue - 7,
Year - 2022
ABSTRACT:
Diagnosis of active tuberculosis (TB) is the first step to determine the next treatment management of patient care. The success of the treatment will not be achieved without the determined accurate diagnosis rapidly. The definitive diagnosis of tuberculosis includes the presence of Mycobacterium tuberculosis through smear staining or histopathological examination or culture method or molecular methods, however some of the methods have limitations for TB diagnosis, especially on extrapulmonary tuberculosis (EPTB). In EPTB, culture method is time consuming till 6-8 weeks, where as it requires a rapid diagnosis, while tissue biopsy to obtain histopathological images is an invasive procedure. Likewise, the constrains on determining diagnosis of pulmonary TB (PTB) especially child TB and EPTB. In certain condition, the detected ADA level exceeds the detection limit as well as in TB disease with an active process. To study the meaning detection of Serum Adenosis Deaminase in serum of patients as an indicator in diagnosing of active disease process in Pulmonary and Extra Pulmonary Tuberculosis. This study was an observational study with a cross sectional study method, carried out in 8 months, by taking serum of blood samples from patients diagnosed with PTB and EPTB. The sample examination, then measuring the ADA serum levels using the ELISA method in Dr Ramelan Hospital Surabaya Indonesia, and cofirmed MTBC in blood samples using standard PCR of gyrB gene region in the Tuberculosis Laboratory, Tropical Disease Institute, Airlangga University, Surabaya, Indonesia. In totally 176 serum samples form PTB and EPTB patients, at January 2020 until December 2020, there were no significant results between serum Adenosine Deaminase (ADA) levels in diagnosing Pulmonary Tuberculosis and Extra Pulmonary Tuberculosis at Dr. Ramelan Hospital Surabaya. So, in this study, ADA serum levels could have the potential role in the diagnosis of pulmonary tuberculosis and EPTB, otherwise this study revealed that ADA serum activity is not a useful test for differentiating tuberculosis from other respiratory diseases, and could be an additional test especially for disease active progressive or recovery.
Cite this article:
Retno Budiarti, Ediono, Ni Made Mertaniasih, Soedarsono. Adenosine Deaminase Detection in Serum of Pulmonary Tuberculosis and Extra Pulmonary Tuberculosis patients at Dr. Ramelan Hospital Surabaya. Research Journal of Pharmacy and Technology. 2022; 15(7):2987-1. doi: 10.52711/0974-360X.2022.00498
Cite(Electronic):
Retno Budiarti, Ediono, Ni Made Mertaniasih, Soedarsono. Adenosine Deaminase Detection in Serum of Pulmonary Tuberculosis and Extra Pulmonary Tuberculosis patients at Dr. Ramelan Hospital Surabaya. Research Journal of Pharmacy and Technology. 2022; 15(7):2987-1. doi: 10.52711/0974-360X.2022.00498 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2022-15-7-20
REFERENCE:
1. Glaziou P, Floyd K, Raviglione MC. Global Epidemiology of Tuberculosis. Semin Respir Crit Care Med. 2018 Jun;39(3):271-285. doi: 10.1055/s-0038-1651492.
2. Lamees M. Al-Janabi. Immunological and Biochemical Changes Related of Tuberculosis in Human. Research J. Pharm. and Tech. 2019; 12(7):3428-3430. doi: 10.5958/0974-360X.2019.00580.8
3. Vijaya Kumar S.,Laxmi Nookala, Surya Prakash, Priya Rajam Vivean. Ziehl-Neelsen (ZN) Stained Method: Presence and Absence of Acid-Fast Bacilli (AFB) of Pulmonary and Non-Pulmonary Tuberculosis Patients Under Went Anti-Tuberculosis Treatment. Research J. Pharm. and Tech. 8(5): 529-532. doi: 10.5958/0974-360X.2015.00088.8
4. Lama Doya, Danial Dalloul, Mohammad Alkhayer, Haissam Yazigi. The importance of diagnosing Mycobacterium tuberculosis by real time PCR Compared with the approved diagnostic methods in the Clinical Laboratory. Research J. Pharm. and Tech 2018; 11(4): 1513-1515. doi: 10.5958/0974-360X.2018.00281.0
5. Barua, R. and Hossain, M. Adenosine Deaminase in Diagnosis of Tuberculosis: A Review. Anwer Khan Modern Medical College Journal. 2014; 5(2), pp. 43–48.
6. Nadim Chhipa, Pinkal Patel, Neil Panchal, Rakesh Parmar. Nitroimidazoles: A newer class of Heterocycles for treatment of Tuberculosis. Research J. Pharm. and Tech. 2020; 13(7): 3425-3432. doi: 10.5958/0974-360X.2020.00609.5
7. Mohammed Shabil, Rajesh V, Bharath Raj K C, Rajesh K S, Prasanna Shama K, Gururaja M P, Himanshu Joshi. A Study on Treatment Defaulters in Tuberculosis Patients on Dots Therapy. Research J. Pharm. and Tech. 2019; 12(5):2245-2253. doi: 10.5958/0974-360X.2019.00374.3
8. Sandip Zine, Shweta A. Patankar, Sushmita S. Raopati. Rise of Antibiotic Resistance in Tuberculosis. Research J. Pharm. and Tech 2018; 11(7): 3201-3204. doi: 10.5958/0974-360X.2018.00588.7
9. Gandikota Thanmayi, P Shanmugasundaram, P. Geetha. Role of Clinical Pharmacist in Improving Patients Compliance including Risk Factors among Tuberculosis Patients. Research J. Pharm. and Tech. 2017; 10(9): 2945-2948. doi: 10.5958/0974-360X.2017.00521.2
10. Rosfadilla P, Widirahardjo, Syarani F, Mutiara E. Diagnostic Accuracy of Pleural Fluid Adenosine Deaminase Level Test in Tuberculous Pleural Effusion. J Respir Indo Vol. 37(4). 2017; p. 278-282
11. Jaya Martolia, Hardik Soni, Falguni Tandel. Natural perspective for Management of drug resistant Tuberculosis: A Review. Research J. Pharm. and Tech. 2020; 13(8):4002-4008. doi: 10.5958/0974-360X.2020.00708.8
12. Golden, M. P. and Vikram, H. R. Extrapulmonary tuberculosis: An overview, American Family Physician. 2005; 72(9), pp. 1761–1768.
13. Amany SH. Jabe, Kadhimmohan Manhi2, Imanhadi Alfayyadh, Saad S. Hamim. Prevalence of Tuberculosis from 2010-2015 in Nasiriyah City/Iraq. Research J. Pharm. and Tech. 2019; 12(5):2275-2278. doi: 10.5958/0974-360X.2019.00379.2
14. Rasheed Ahemad Shaik, Syed Jaffer, Syeda Humera Fatima. Study on Prevalence of Adverse Drug Reactions and Drug-Drug Interactions and Co-Morbid Conditions of Patients Suffering from Tuberculosis. Research Journal of Pharmacy and Technology. 2021; 14(7):3911-5. doi: 10.52711/0974-360X.2021.00679
15. Aggarwal AN, Agarwal R, Sehgal IS, Dhooria S. Adenosine deaminase for diagnosis of tuberculous pleural effusion: A systematic review and meta-analysis. PLoS One. 2019; 14(3):e0213728. doi: 10.1371/journal.pone.0213728. PMID: 30913213; PMCID: PMC6435228.
16. Liang QL, Shi HZ, Wang K, Qin SM, Qin XJ. Diagnostic accuracy of adenosine deaminase in tuberculous pleurisy: a meta-analysis. Respir Med. 2008; 102(5): 744-54. doi: 10.1016/j.rmed.2007.12.007.
17. Parish ST, Kim S, Sekhon RK, Wu JE, Kawakatsu Y, Effros RB. Adenosine deaminase modulation of telomerase activity and replicative senescence in human CD8 T lymphocytes. J Immunol. 2010; 184(6): 2847-54. doi: 10.4049/jimmunol.0903647.
18. van Crevel R, Ottenhoff TH, van der Meer JW. Innate immunity to Mycobacterium tuberculosis. Clin Microbiol Rev. 2002; 15(2): 294-309. doi: 10.1128/CMR.15.2.294-309.2002.
19. Zhou XX, Liu YL, Zhai K, Shi HZ, Tong ZH. Body Fluid Interferon-γ Release Assay for Diagnosis of Extrapulmonary Tuberculosis in Adults: A Systematic Review and Meta-Analysis. Sci Rep. 2015; 5:15284. doi: 10.1038/srep15284.
20. Saranya, Parthasarathy V, Hariprasad B, Shobha Rani H. Factors Influencing Rifampicin Autoinduction in Adult Pulmonary Tuberculosis Patients. Research J Pharm and Tech. 2016; 9(8): 1223-1228. doi: 10.5958/0974-360X.2016.00233.X