Author(s):
Aasem Mohamed Al-Byti, Saygin Abdulkadir Chakmakchy, Abdulrazzaq Abbas Waheeb, Avan hassan Mohammed Ameen, Muhannad Abdullah Alazzawy, Nihad Khalawe Tektook
Email(s):
drnihadkhalawe@gmail.com
DOI:
10.52711/0974-360X.2022.00537 
Address:
Aasem Mohamed Al-Byti1, Saygin Abdulkadir Chakmakchy2, Abdulrazzaq Abbas Waheeb3, Avan hassan Mohammed Ameen4, Muhannad Abdullah Alazzawy5, Dr. Nihad Khalawe Tektook6
1-3M.B.Ch. B., FICM (Plastic), Tikrit University
College of Medicine, Tikrit, Iraq.
4M.B.Ch. B., FICM (Plastic), Kirkuk University
College of Medicine, Kirkuk, Iraq.
5Ph. D. Medical Microbiology, Kirkuk Health Directorate.
6Middle Technical University/College of Medical and Health Technology, Medical laboratory techniques dep. Iraq.
*Corresponding Author
Published In:
Volume - 15,
Issue - 7,
Year - 2022
ABSTRACT:
A total of 110 wound swabs were collected from the beginning of February 2020 to the end of April 2021 from patients attending private plastic surgery clinics. Collection of vaginal swabs included cultivation on blood agar and MacConkey agar for 24 hour to evaluate the role of S aureus in surgical infection associated with elective rhinoplasty. Media were organized and purified by the producer's direction. The prepared media were used for separation, affirmation of the useful check, conspicuous confirmation and weakness testing these media were finished in the wake of being solidified. Swabs was inoculated onto MacConkey, supplement and blood agars. By then the vaccinated plates were brought forth at 37°C for 24 hr. Inoculum from the attempted bacterium was prepared. A single territory was moved to a by sterile q-tip is dove into the inoculum and subsequently cleaned consistently over the outside of a Muller-Hinton agar plate, after that inside 15 minutes of inoculation, the antimicrobial-containing circles are applied to the agar with a forceps crushed determinedly to ensure contact with agar and a while later plate switched and brought forth at 37oC for 18 hours. According to distribution of the positive culture, the highest percentage of positive wound culture were within the age group 16-25 year (37.04%), followed by 25-35 yea (25.93%). According to the distribution of the isolated bacteria among the study groups, the common isolated bacteria from wound infection was S. aureus with rate (62.96%), followed by E.coli(14.81%) and the lowest rate was with K. pneumoniae, Pseudomonas aeruginosa and S epidermidis. Regarding to the virulence factors of Staphylococcus aureus, the study showed that, all S. aureus isolates were coagulase and DNase positive, 94.11% of isolates were beta-hemolysis, 88.23% were characterized by invasiveness (Growth on congored Agar), 82.35% of isolates was lecithinase and capsule production while 29.41% were protease production with highly significant relation between Staphylococcus aureus and in wound infection. the high rate of vancomycin resistance as virulence factors was found among S. aureus isolates (11.76%). Beta-lactam and methicillin resistances was recorded highly among S. aureus isolates (70.58%)
Cite this article:
Aasem Mohamed Al-Byti, Saygin Abdulkadir Chakmakchy, Abdulrazzaq Abbas Waheeb, Avan hassan Mohammed Ameen, Muhannad Abdullah Alazzawy, Nihad Khalawe Tektook. Study of S. aureus isolated from wound infection of Rhinoplasty. Research Journal of Pharmacy and Technology. 2022; 15(7):3205-8. doi: 10.52711/0974-360X.2022.00537
Cite(Electronic):
Aasem Mohamed Al-Byti, Saygin Abdulkadir Chakmakchy, Abdulrazzaq Abbas Waheeb, Avan hassan Mohammed Ameen, Muhannad Abdullah Alazzawy, Nihad Khalawe Tektook. Study of S. aureus isolated from wound infection of Rhinoplasty. Research Journal of Pharmacy and Technology. 2022; 15(7):3205-8. doi: 10.52711/0974-360X.2022.00537 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2022-15-7-59
REFERENCES:
1. Allegranzi B, Nejad SB, Combescure C, Graafmans W, Attar H, Donaldson L, Pittet D. Burden of endemic health-care-associated infection in developing countries: systematic review and meta-analysis. Lancet. 2011; 14(9761):228–241.
2. Lisboa T, Faria M, Hoher JA, Borges LAA, Gómez J, Schifelbain L, Dias FS, Lisboa J, Friedman G. Prevalência de Infecção Nosocomial em Unidades de Terapia Intensiva do Rio Grande do Sul. Rev Bras Terapia Intensiva. 2007; 14(4):414–420.
3. Borgundvaag B, Ng W, Rowe B, Katz K. Prevalence of methicillin-resistant Staphylococcus aureus in skin and soft tissue infections in patients presenting to Canadian emergency departments. CJEM. 2013; 14(3):141–160.
4. Lipsky BA, Weigelt JA, Gupta V, Killian A, Peng MM. Skin, soft tissue, bone, and joint infections in hospitalized patients: epidemiology and microbiological, clinical, and economic outcomes. Infect Control Hosp Epidemiol. 2007; 14(11):1290–1298.
5. Zervos MJ, Freeman K, Vo L, Haque N, Pokharna H, Raut M, Kim M. Epidemiology and outcomes of complicated skin and soft tissue infections in hospitalized patients. J Clin Microbiol. 2012; 14(2):238–245. doi: 10.1128/JCM.05817-11.
6. Albaugh KW, Biely SA, Cavorsi JP. The effect of a cellulose dressing and topical vancomycin on methicillin-resistant Staphylococcus aureus (MRSA) and Gram-positive organisms in chronic wounds: a case series. Ostomy Wound Manage. 2013; 14(5):34–43.
7. Aragónchez J, Lipsky BA, Lázaro-Martínez JL. Gram-negative diabetic foot osteomyelitis: risk factors and clinical presentation. Int J Low Extrem Wounds. 2013; 14(1):63–68. doi: 10.1177/1534734613477423.
8. Bessa LJ, Fazii P, Di Giulio M, Cellini L. Bacterial isolates from infected wounds and their antibiotic susceptibility pattern: some remarks about wound infection. Int Wound J. 2013. doi:10.1111/iwj.12049.
9. Feleke Y, Mengistu Y, Enquselassie F. Diabetic infections: clinical and bacteriological study at Tikur Anbessa Specialized University Hospital, Addis Ababa, Ethiopia. Ethiop Med J. 2007; 14(2):171–179.
10. Trividic-Rumeau M, Bouyssou-Gauthier ML, Mounier M, Sparsa A, Blaise S, Bédane C, Bonnetblanc JM. Methicilline-sensitive and methicilline-resistant Staphylococcus aureus related morbidity in chronic wounds: a prospective study. Ann Dermatol Venereol. 2003; 14(6–7):601–605.
11. Lima AF, Costa LB, Silva JL, Maia MB, Ximenes EC. Interventions for wound healing among diabetic patients infected with Staphylococcus aureus: a systematic review. Sao Paulo Med J. 2011; 14(3):165–170.
12. Sisirak M, Zvizdic A, Hukic M. Methicillin-resistant Staphylococcus aureus (MRSA) as a cause of nosocomial wound infections. Bosn J Basic Med Sci. 2010; 14(1):32–37.
13. Abdallah SA, Al-Asfoor KK, Salama MF, Al-Awadi BM. Prospective analysis methicillin-resistant staphylococcus aureus and its risk factors. J Glob Infect Dis. 2013; 14(1):19–25. doi: 10.4103/0974-777X.107170.
14. McKinnell JA, Huang SS, Eells SJ, Cui E, Miller LG. Quantifying the impact of extranasal testing of body sites for methicillin-resistant Staphylococcus aureus colonization at the time of hospital or intensive care unit admission. Infect Control Hosp Epidemiol. 2013; 14(2):161–170.
15. Richard JL, Sotto A, Jourdan N, Combescure C, Vannereau D, Rodier M, Lavigne JP. Risk factors and healing impact of multidrug-resistant bacteria in diabetic foot ulcers. Diabetes Metab. 2008; 14(4):363–369.
16. Madani TA. Epidemiology and clinical features of methicillin-resistant Staphylococcus aureus in the University Hospital, Jeddah, Saudi Arabia. Can J Infect Dis. 2002; 14(4):245–250. ]
17. Tosh PK, Agolory S, Strong BL, Verlee K, Finks J, Hayakawa K, Chopra T, Kaye KS, Gilpin N, Carpenter CF, Haque NZ, Lamarato LE, Zervos MJ, Albrecht VS, McAllister SK, Limbago B, Maccannell DR, McDougal LK, Kallen AJ, Guh AY. Prevalence and risk factors associated with vancomycin-resistant staphylococcus aureus precursor organism colonization among patients with chronic lower-extremity wounds in Southeastern Michigan. Infect Control Hosp Epidemiol. 2013; 14(9):954–960.]
18. Ramarathnam V, De Marco B, Ortegon A, Kemp D, Luby J, Sreeramoju P. Risk factors for development of methicillin-resistant Staphylococcus aureus infection among colonized patients. Am J Infect Control. 2013; 14(7):625–628.
19. Maina EK, Kiiyukia C, Wamae CN, Waiyaki PG, Kariuki S. Characterization of methicillin-resistant Staphylococcus aureus from skin and soft tissue infections in patients in Nairobi, Kenya. Int J Infect Dis. 2013; 14(2):e115–e119.
20. Edris B, Reed JF. MRSA infection in lower extremity wounds. Int J Low Extrem Wounds. 2008; 14(1):28–31.
21. IBGE. Instituto Brasileiro de Geografia e Estatística: Síntese de indicadores sociais: uma análise das condições de vida da população brasileira 2012. Estud Pesquisas Inform Demogr Soc. 2012; 14:293.
22. The Clinical and Laboratory Standards Institute (CLSI) Performance standards for antimicrobial susceptibility testing, twenty-third informational supplement. Wayne PA: Document M100-S23; 2013.
23. Pacheco AB, Guth BE, Soares KC, Nishimura L, De Almeida DF, Ferreira LC. Random amplification of polymorphic DNA reveals serotype-specific clonal clusters among enterotoxigenic Escherichia coli strains isolated from humans. J Clin Microbiol. 1997; 14(6):1521–1525.