ABSTRACT:
Plants bioactive have been found to be useful in therapeutic management of various diseases such as Cancer, Inflammation and diabetes. Analysis part becomes difficult when use their combination Analysis forms essential exercise before any formulation development. Various analytical techniques such as Chromatographic, Electrochemical and Spectrophotometric have been applied in the analysis of pharmaceuticals. However chromatographic methods are time consuming processes for separation of compounds and costly to these as require high quantity of solvent. Moreover, electrochemical methods are sensitive to temperature. Herein simple, rapid, economical efficient method has been reported for simultaneous estimation of curcumin and quercetin. This simultaneous equation method or Vierordt’s method involves use of methanol as solvent and further dilutions were made using phosphate buffer pH6.8 and were measured at two wavelengths 324nm and 426nm. The linearity was noted in concentration range of 2-12µg/ml for both the bioactives. Calibration curves of both were found to be linear with regression coefficient (r2) values as 0.991 for Quercetin and 0.999 for Curcumin. Linearity, accuracy and precision was validated for this method as per ICH guidelines. The noted values of accuracy, precision and other statistical analysis were found to be in good accordance with the prescribed values. UV-Spectrophotometric method was proposed and validated for simultaneous quantification of both Curcumin and Quercetin throughout formulation progress presently initiated in our laboratories. The proposed analytical method is easy, economical and experimentally prevents loss of time.