Author(s): Magda Rosalina Hutagalung, David S. Perdanakusuma, Pratidina Wulandari

Email(s): wulan_tox@yahoo.com

DOI: 10.52711/0974-360X.2022.00597   

Address: Magda Rosalina Hutagalung, David S. Perdanakusuma, Pratidina Wulandari*
Department of Plastic Reconstructive and Aesthetic Surgery, Faculty of Medicine, Universitas Airlangga, Dr. Soetomo General Hospital, Surabaya, 60115, Indonesia.
*Corresponding Author

Published In:   Volume - 15,      Issue - 8,     Year - 2022


ABSTRACT:
Imbalance of collagen synthesis and degradation causes formation of abnormal scarring leading to hypertrophic scar or keloid. Previous studies have shown that applying antihistamines could reduce the level of dermal collagen. However, no data whether topical antihistamine could be used as preventative therapy for abnormal scar formation. The aim of this study was to assess whether the application of topical antihistamine in acute wounds could affect the level of transforming growth factor beta (TGF-ß), a regulator of wound healing process, and collagen in wound healing phase in order to suppress the keloid formation. A randomized post-test only control group design study was conducted. Twenty-four acute wounds on Rattus novergicus rats were randomized divided into control and treatment groups. The animals of treatment group received daily topical antihistamine consisting 1% diphenhydramine hydrochloride and 0.1% zinc acetate. The full-thickness skin tissue from each rat were taken on day 5 and 21 and were subjected to ELISA test to measure the levels of TGF-ß and collagen. Our data found that in the treatment group, the level of collagen decreased significantly over time (1623.61ng/mL on day 5 to 755.15ng/mL on day 21, p=0.001). Similarly, the level of TGF-? also decreased although was not statistically significant (p=0.766). In contrast, the levels of TGF-ß level and collagen in the control group increased significantly from day 5 to day 21, p=0.003 and p=0.001, respectively. The level of TGF-? in treatment group was significantly higher compared to the control group p=0.001 on day 21 while the level of collagen had no different between treatment and control group (p=0.124). In conclusion, our data suggest that topical antihistamine reduced the levels of TGF-ß and collagen at the end of the proliferation phase or the beginning of the remodeling phase. Further study is warrant to elucidate the effect of antihistamine on other would healing markers or parameters.


Cite this article:
Magda Rosalina Hutagalung, David S. Perdanakusuma, Pratidina Wulandari. Effect of Topical H1-antihistamine on the level of Transforming growth factor beta (TGF-β) and Collagen of Acute wound in animal model. Research Journal of Pharmacy and Technology. 2022; 15(8):3559-2. doi: 10.52711/0974-360X.2022.00597

Cite(Electronic):
Magda Rosalina Hutagalung, David S. Perdanakusuma, Pratidina Wulandari. Effect of Topical H1-antihistamine on the level of Transforming growth factor beta (TGF-β) and Collagen of Acute wound in animal model. Research Journal of Pharmacy and Technology. 2022; 15(8):3559-2. doi: 10.52711/0974-360X.2022.00597   Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2022-15-8-39


REFERENCES:
1.    Winter GD. Some factors affecting skin and wound healing. J Tissue Viability. 2006; 16:20-3.
2.    Guo S, Dipietro LA. Factors affecting wound healing. J Dent Res. 2010; 89:219-29.
3.    Wang XJ, Han G, Owens P, Siddiqui Y, Li AG. Role of TGF beta-mediated inflammation in cutaneous wound healing. J Investig Dermatol Symp Proc. 2006; 11:112-7.
4.    Ramirez H, Patel SB, Pastar I. The Role of TGFbeta Signaling in Wound Epithelialization. Adv Wound Care (New Rochelle). 2014; 3:482-91.
5.    Penn JW, Grobbelaar AO, Rolfe KJ. The role of the TGF-beta family in wound healing, burns and scarring: a review. Int J Burns Trauma. 2012; 2:18-28.
6.    Perdanakusuma D, Noer M. Penanganan parut hipertrofik dan keloid. Surabaya: Airlangga University Press; 2006.
7.    Albaugh VL, Mukherjee K, Barbul A. Proline Precursors and Collagen Synthesis: Biochemical Challenges of Nutrient Supplementation and Wound Healing. J Nutr. 2017; 147:2011-7.
8.    Cohen IK, Keiser HR, Sjoerdsma A. Collagen synthesis in human keloid and hypertrophic scar. Surg Forum. 1971; 22:488-9.
9.    Cohen IK, Keiser HR. Collagen synthesis in keloid and hypertrophic scar following intralesional use of triamcinolone. Surg Forum. 1973; 24:521-23.
10.    Cohen IK, Beaven MA, Horakova Z, Keiser HR. Histamine and collagen synthesis in keloid and hypertrophic scar. Surg Forum. 1972; 23:509-10.
11.    Zhou S, Salisbury J, Preedy VR, Emery PW. Increased collagen synthesis rate during wound healing in muscle. PLoS One. 2013; 8:e58324.
12.    Taman EA, Aamer A. Recurrent earlobe keloids: a combined intralesional injection of 5-fluorouracil and excision. Egyptian Journal of Dermatology and Venerology. 2015; 35:23.
13.    Patil SR, Patil S, Patil MB, Kumar R, Paschapur MS. Screening For Wound Healing Activity of Alcoholic Extract of Ocimum kilimandscharicum Leaves. Research Journal of Pharmacology and Pharmacodynamics. 2009; 1:93-6.
14.    Badgujar SB, Mahajan RT, Chopda MZ. Wound healing activity of latex of Euphorbia nivulia Buch.-ham. in mice. Research Journal of Pharmacology and Pharmacodynamics. 2009; 1:90-2.
15.    Murti K, Lambole V, Panchal M, Shah M, Gajera V. Evaluation of wound healing activity of polyherbal formulation in rats. Res J Pharmacogn Phytochem. 2011; 3:112-5.
16.    Kumar KT, Panda DS, Khuntia S. Evaluation of Wound Healing and Anti-Oxidant Property of Seed Oil of Morinda citrifolia L (Noni) In Wistar Rats. Research Journal of Pharmacognosy and Phytochemistry. 2010; 2:203-7.
17.    Panda P, Ghosh A, Panda M. Anti-inflammatory and wound healing activity of Eupatorium odoratum Linn. Leaves. Research Journal of Pharmacognosy and Phytochemistry. 2010; 2:211-6.
18.    Khare P, Goswami RB, Khare S, Pathak AK. Evaluation of Wound Healing Activity of Ageratum conyzoides Linn. Research Journal of Pharmacognosy and Phytochemistry. 2009; 1:217-9.
19.    Elayaraja A, Vijayalakshmi M, Garikapati D. Evaluation of Wound Healing Potential of Ethanolic Extracts of Various Parts of Phyllanthus simplex Retz. Research Journal of Pharmacognosy and Phytochemistry. 2010; 2:34-6.
20.    Kumar D, Prasad DN, Dua JS, Bhatnagar SP. Evaluation of Wound Healing Activity of Ethanolic Extract of Aerva tomentosa Forsk. Research Journal of Pharmacognosy and Phytochemistry. 2009; 1:188-90.
21.    Ghori SS, Gouse M, Panda N, Khaled S, Basheeruddin S, Baig MD, et al. Wound Healing Activity of Poly Herbal formulation. Research Journal of Pharmaceutical Dosage Forms and Technology. 2015; 7:125-8.
22.    Purohit SK, Solanki R, Mathur V, Mathur M. Evaluation of wound healing activity of ethanolic extract of Curcuma longa rhizomes in male albino rats. Asian Journal of Pharmaceutical Research. 2013; 3:79-81.
23.    Daphne TL, Prince V. A Study to assess the Effectiveness of Aloe Vera Gel Dressing on Wound Status among Patients with Diabetes Mellitus in Government Hospital, Dindigul. International Journal of Advances in Nursing Management. 2019; 7:159-62.
24.    Bura AR. Effect of Wound Healing Potential of Plumeria obtusa (Champa) Spray. Asian Journal of Pharmaceutical Research. 2018; 8:231-5.
25.    Amminbavi D, Lakshmi NP. Assessment of In vitro wound healing potential of Hibiscus leaf extract Emulgel. Asian Journal of Pharmaceutical Research. 2020; 10.
26.    Zigron A, Dror AA, Morozov NG, Shani T, Haj Khalil T, Eisenbach N, et al. COVID-19 Vaccine Acceptance Among Dental Professionals Based on Employment Status During the Pandemic. Front Med (Lausanne). 2021; 8:618403.
27.    Rawat S, Gupta A. Development and study of wound healing activity of an ayurvedic formulation. Asian J Res Pharm Sci. 2011; 1:26-8.
28.    Purohit SK, Solanki R, Soni R, Mathur V. Evaluation of wound healing activity of ethanolic extract of Azadirachta indica leaves in male albino rats. Asian Journal of Pharmacy and Technology. 2013; 3:73-5.
29.    Badmanaban R, Patel CN, Devi P, Meera R, Kameswari B, Eswarapriya B. Physio-Chemical investigation and Wound healing activity of Sesame oil and Formulated oil. Asian Journal of Research in Chemistry. 2009; 2:501-5.
30.    Jain S, Jain N, Tiwari A, Balekar N, Jain DK. Simple evaluation of wound healing activity of polyherbal formulation of roots of Ageratum conyzoides Linn. Asian Journal of Research in Chemistry. 2009; 2:135-8.
31.    Vijayabhaskar K, Sravanprasad M, Venkateshwarlu G, Devi PS, Kumar KH, Sunil J. Wound Healing Activity of Bauhinia purpurea in Albino Wistar Rats. Asian Journal of Research in Pharmaceutical Science. 2011; 1:47-9.
32.    Jailani M, Perdanakusuma D. Peran Histamin dan Antihistamin pada Sintesis Kolagen. Universitas Airlangga. 2001; Surabaya.
33.    Venugopal J, Ramakrishnan M, Habibullah CM, Babu M. The effect of the anti-allergic agent avil on abnormal scar fibroblasts. Burns. 1999; 25:223-8.
34.    Liu H, Zheng Q, Farley JM. Antimuscarinic actions of antihistamines on the heart. J Biomed Sci. 2006; 13:395-401.
35.    Farzam K, Sabir S, O'Rourke MC. Antihistamines.  StatPearls. Treasure Island (FL)2021.

Recomonded Articles:

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

1.3
2021CiteScore
 
56th percentile
Powered by  Scopus


SCImago Journal & Country Rank


Recent Articles




Tags


Not Available