Author(s): Saif M. Dmour, Haitham Qaralleh, Muhamad Al-Limoun, Khaled M. Khleifat, Moath Alqaraleh, Ali Abdallah Alqudah, Rakan M. Altarawneh


DOI: 10.52711/0974-360X.2022.00654   

Address: Saif M. Dmour1*, Haitham Qaralleh2, Muhamad Al-Limoun3, Khaled M. Khleifat4, Moath Alqaraleh5, Ali Abdallah Alqudah6, Rakan M. Altarawneh7
1Department of Laboratory Medical Sciences, Princess Aisha Bent Al-Hussein Faculty of Nursing and Health Sciences, Al Hussein bin Talal University, Jordan.
2Department of Medical Laboratory Sciences, Mutah University, Mutah, Karak, Jordan.
3Biology Department, Mutah University, Mutah, Karak, Jordan.
4Faculty of Allied Medical Sciences, Al-Ahliyya Amman University, Amman, Jordan.
5Pharmacological and Diagnostic Research Center (PDRC), Faculty of Pharmacy, Al-Ahliyya Amman University, Amman 19328, Jordan.
6Department of Applied Biology, Tafila Technical University, PO Box 179, 66110 Tafila, Jordan.
7Department of Chemistry, Faculty of Science, Mu'tah University, Al-Karak, Jordan.
*Corresponding Author

Published In:   Volume - 15,      Issue - 9,     Year - 2022

Resistance to antibiotics is a significant public health issue in preventing infectious diseases. This study was designed to evaluate the antibacterial activity of eucalyptol, ?-terpinene, p-cymol and punicalagin against Methicillin (Oxacillin) resistant strains. The synergistic effect of these compounds with cefotaxime against Staphylococcus aureus was investigated. The lowest MIC value observed was 0.08mg/mL for punicalagin against S. aureus, followed by the MIC values of punicalagin against E. aerogenes, E. coli and K. pneumoniae (0.16 - 0.63mg/mL). The activity of p-cymol against S. aureus is ranked second, as indicated by the MIC value (0.63mg/mL). The results of the combination study showed that the maximum Increase in Folding Area (IFA) was reported when punicalagin was combined with cefoxitin, cefotaxime, oxacillin, and piperacillin. An effective IFA was observed when p-cymol was tested with piperacillin, cefixime, cefotaxime, oxacillin, and cefoxitin. Based on the checkerboard assay, the MIC of cefotaxime decreased from 60 to 7.5µg/mL when combined with punicalagin indicating a significant synergistic effect between cefotaxime and punicalagin against S. aureus. In conclusion, punicalagin improved S. aureus susceptibility to cefotaxime, suggesting that using these two medicines together can reverse beta-lactam resistance in methicillin (oxacillin) resistant S. aureus. Further works are required to generalize this result and then it may be useful for treating diseases caused by methicillin (oxacillin) resistant strains.

Cite this article:
Saif M. Dmour, Haitham Qaralleh, Muhamad Al-Limoun, Khaled M. Khleifat, Moath Alqaraleh, Ali Abdallah Alqudah, Rakan M. Altarawneh. Combined Antibacterial activity of Eucalyptol, γ-terpinene, p-cymol and punicalagin with Cefotaxime against Methicillin (Oxacillin) Resistant Staphylococcus aureus Isolate. Research Journal of Pharmacy and Technology. 2022; 15(9):3905-1. doi: 10.52711/0974-360X.2022.00654

Saif M. Dmour, Haitham Qaralleh, Muhamad Al-Limoun, Khaled M. Khleifat, Moath Alqaraleh, Ali Abdallah Alqudah, Rakan M. Altarawneh. Combined Antibacterial activity of Eucalyptol, γ-terpinene, p-cymol and punicalagin with Cefotaxime against Methicillin (Oxacillin) Resistant Staphylococcus aureus Isolate. Research Journal of Pharmacy and Technology. 2022; 15(9):3905-1. doi: 10.52711/0974-360X.2022.00654   Available on:

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