Author(s): Nur Fitriana Muhammad Ali, Irnawati, Egha Rina, Dwiprayogo Wibowo, Andi Nafisah Tendri Adjeng


DOI: 10.52711/0974-360X.2022.00659   

Address: Nur Fitriana Muhammad Ali1, Irnawati2, Egha Rina2, Dwiprayogo Wibowo1, Andi Nafisah Tendri Adjeng3*
1Department of Pharmacy, Faculty of Science and Technology Institute Teknologi dan Kesehatan AVICENNA, Jl. Y. Wayong By Pass Lepo-Lepo, Kota Kendari, Sulawesi Tenggara, Indonesia, 93116.
2 Department of Pharmacy, Faculty of Pharmacy, Universitas Halu Oleo, Kendari, South-East Sulawesi, 93232, Indonesia.
3Department of Pharmacy, Faculty of Medicine, Universitas Lampung, Bandar Lampung 35145, Indonesia.
*Corresponding Author

Published In:   Volume - 15,      Issue - 9,     Year - 2022

The object of this study was aimed to determine the antifungal activity of papaya seeds (Carica papaya L.) ethanol extract against the fungi Candida albicans. The research method was experimental research consisting of several stages of sample processing, extraction, phytochemical screening, and tested antifungal activity of ethanol extract of papaya seeds young. Screening of Phytochemical results showed that ethanol extract of papaya seeds contains flavonoids, alkaloids, tannins, steroids and saponins. Test antifungal activity carried out by the agar diffusion method (pitting) was by measuring the inhibition zone formed around the wells. The test results showed that the antifungal activity of ethanol extract of papaya seeds had antifungal activity against Candida albicans at 0.1%, 0.3%, 0.6% and 1% in diameter inhibition zone respectively by 11mm; 12mm; 13mm; and 14 mm and are included in the category of strong inhibition.

Cite this article:
Nur Fitriana Muhammad Ali, Irnawati, Egha Rina, Dwiprayogo Wibowo, Andi Nafisah Tendri Adjeng. Qualitative Phytochemical Screening and Antifungal Activity of Ethanol Extract of Young Papaya Seeds (Carica papaya L.) against Candida albicans. Research Journal of Pharmacy and Technology. 2022; 15(9):3936-0. doi: 10.52711/0974-360X.2022.00659

Nur Fitriana Muhammad Ali, Irnawati, Egha Rina, Dwiprayogo Wibowo, Andi Nafisah Tendri Adjeng. Qualitative Phytochemical Screening and Antifungal Activity of Ethanol Extract of Young Papaya Seeds (Carica papaya L.) against Candida albicans. Research Journal of Pharmacy and Technology. 2022; 15(9):3936-0. doi: 10.52711/0974-360X.2022.00659   Available on:

1.    Silva S, Negri M, Henriques M, Oliveira R, Williams DW, Azeredo J. Candida glabrata, Candida parapsilosis and Candida tropicalis : biology, epidemiology, pathogenicity and antifungal resistance. FEMS Microbiology Reviews. 2012; 36(2):288-305. doi:10.1111/j.1574-6976.2011.00278.x
2.    Pal M. Morbidity and mortality due to fungal infections. J Appl Microbiol Biochem. 2017; 1:1-3. doi:10.21767/2576-1412.100002
3.    Wozniak KL, Wormley FL, Fidel PL. Candida-specific antibodies during experimental vaginal candidiasis in mice. Infection and Immunity. 2002; 70(10):5790-5799. doi:10.1128/IAI.70.10.5790-5799.2002
4.    Elango G, Roopan SM, Al-Dhabi NA, Arasu MV, Dhamodaran KI, Elumalai K. Coir mediated instant synthesis of Ni-Pd nanoparticles and its significance over larvicidal, pesticidal and ovicidal activities. Journal of Molecular Liquids. 2016; 223:1249-1255. doi:10.1016/j.molliq.2016.09.070
5.    Elango G, Mohana Roopan S, Abdullah Al-Dhabi N, Arasu MV, Irukatla Damodharan K, Elumalai K. Cocos nucifera coir-mediated green synthesis of Pd NPs and its investigation against larvae and agricultural pest. Artificial Cells, Nanomedicine, and Biotechnology. 2017; 45(8):1581-1587. doi:10.1080/21691401.2016.1262382
6.    Fowsiya J, Madhumitha G, Al-Dhabi NA, Arasu MV. Photocatalytic degradation of Congo red using Carissa edulis extract capped zinc oxide nanoparticles. Journal of Photochemistry and Photobiology B: Biology. 2016; 162:395-401. doi:10.1016/j.jphotobiol.2016.07.011
7.    Abdullah Al-Dhabi N, Valan Arasu M, Rejiniemon TS. In vitro antibacterial, antifungal, antibiofilm, antioxidant, and anticancer properties of isosteviol isolated from endangered medicinal plant Pittosporum tetraspermum. Evidence-Based Complementary and Alternative Medicine. 2015; 2015. doi:10.1155/2015/164261
8.    Balamurugan R. Smilax chinensis Linn.(Liliaceae) root attenuates insulin resistance and ameliorate obesity in high diet induced obese rat. South Indian Journal of Biological Sciences. 2015; 1(1):47-51. doi:10.22205/sijbs/2015/v1/i1/100443
9.    Arokiyaraj S, Saravanan M, Badathala V. Green synthesis of Silver nanoparticles using aqueous extract of Taraxacum officinale and its antimicrobial activity. South Indian J Biol Sci. 2015; 2:115-118. doi:10.22205/sijbs/2015/v1/i2/100433
10.    Barathikannan K, Venkatadri B, Khusro A, et al. Chemical analysis of Punica granatum fruit peel and its in vitro and in vivo biological properties. BMC Complementary and Alternative Medicine. 2016; 16(1):1-10. doi:10.1186/s12906-016-1237-3
11.    Ferreira MP, Palmer J, McKenna EB, Gendron F. A Traditional Elder’s Anti-Aging Cornucopia of North American Plants. In: Foods and Dietary Supplements in the Prevention and Treatment of Disease in Older Adults. Elsevier Inc.; 2015:3-11. doi:10.1016/B978-0-12-418680-4.00001-4
12.    Chávez-Quintal P, González-Flores T, Rodríguez-Buenfil I, Gallegos-Tintoré S. Antifungal activity in ethanolic extracts of Carica papaya L. cv. Maradol leaves and seeds. Indian Journal of Microbiology. 2011; 51(1):54-60. doi:10.1007/s12088-011-0086-5
13.    Sharma A, Bachheti A, Sharma P, Bachheti RK, Husen A. Phytochemistry, pharmacological activities, nanoparticle fabrication, commercial products and waste utilization of Carica papaya L.: A comprehensive review. Current Research in Biotechnology. 2020; 2:145-160. doi:10.1016/j.crbiot.2020.11.001
14.    Iordănescu OA, Băla M, Zippenfening SE, et al. A DPPH· kinetic approach on the antioxidant activity of various parts and ripening levels of papaya (Carica papaya L.) ethanolic extracts. Plants. 2021; 10(8):1679. doi:10.3390/plants10081679
15.    Galih Purwaningdyah Y, Widyaningsih TD, Wijayanti N. Effectiveness of Papaya Seed Extract (Carica Papaya L) as Antidiarrheal in Mice Were Induced Salmonella Typhimurium. Vol 3.; 2015. doi:
16.    Indonesia DK. Parameter standar umum ekstrak tumbuhan obat. Jakarta: Departemen Kesehatan Republik Indonesia. Published online 2000:9-12.
17.    Hammuel C, Anyim BP, Raplong HH, Inekwe VU, Batari ML. Phytochemical screening and in vitro antibacterial activity of methanol and aqueous extracts of Acalypha racemosa leaves. Research Journal of Pharmacognosy and Phytochemistry. 2014; 3(6):118-121. doi:10.13170/aijst.3.2.1486
18.    Suryani, Zubaydah WOS, Sahumena MH, et al. Preparation and characterization of self-nanoemulsifying drug delivery system (SNEDDS) from Moringa oleifera L. and Cassia alata L. leaves extracts. In: AIP Conference Proceedings. Vol 2199. AIP Publishing LLC; 2019:70011. doi:10.1063/1.5141325
19.    Ruskin SR, Ajina S. Qualitative Phytochemical Screening and In-vitro Anthelmintic Activity of Adhatoda vasica (Acanthaceae). Research Journal of Pharmacy and Technology. 2017; 10(2):414-420. doi:10.5958/0974-360X.2017.00083.X
20.    Velavan S. Phytochemical techniques-a review. World Journal of Science and Research. 2015; 1(2):80-91.
21.    Balamurugan G, Arunkumar MP, Muthusamy P, Anbazhagan S. Preliminary Phytochemical Screening, Free radical Scavenging and Antimicrobial activities of Justicia tranquebariensis Linn. Research Journal of Pharmacy and Technology. 2008; 1(2):116-118.
22.    Iskandar Y, Halimah E, Shifa N, Fushilla Z, Silvana G, Zuhrotun A. Activity of Red Algae (Eucheuma Cottonii) against some Bacteria and Fungi. Journal of Pharmaceutical Sciences and Research. 2019; 11(6):2362-2366.
23.    Indonesia R. Departemen Kesehatan. Sediaan galenik, Departemen Kesehatan, Republik Indonesia. Published Online 1986.
24.    Tiwari P, Kumar B, Kaur M, Kaur G, Kaur H. Phytochemical screening and extraction: a review. Internationale Pharmaceutica Sciencia. 2011; 1(1):98-106.
25.    Tiwari A, Singh R, Tripathi RC. Phytochemical Screening and Antibacterial Activity of Terminalia arjuna. Research Journal of Pharmacology and Pharmacodynamics. 2017; 9(3):147-151. doi:10.5958/2321-5836.2017.00025.8
26.    Febriza A, Faradiana S, Yusbar Y, Kasim VN. Antifungal Effects of Solanum Melongena L Peel Extract Against Candida Albicans: In Vitro Study. Published Online 2021.
27.    Del Rio M, de la Canal L, Pinedo M, Mora-Montes HM, Regente M. Effects of the binding of a Helianthus annuus lectin to Candida albicans cell wall on biofilm development and adhesion to host cells. Phytomedicine. 2019; 58:152875. doi:10.1016/j.phymed.2019.152875
28.    Li N, Tan S nan, Cui J, et al. PA-1, a novel synthesized pyrrolizidine alkaloid, inhibits the growth of Escherichia coli and Staphylococcus aureus by damaging the cell membrane. The Journal of Antibiotics. 2014; 67(10):689-696.
29.    Łabieniec M, Gabryelak T. Oxidatively modified proteins and DNA in digestive gland cells of the fresh-water mussel Unio tumidus in the presence of tannic acid and its derivatives. Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 2006; 603(1):48-55. doi:10.1016/j.mrgentox.2005.10.013
30.    Labieniec M, Biernat M, Gabryelak T. Response of digestive gland cells of freshwater mussel Unio tumidus to phenolic compound exposure in vivo. Cell Biology International. 2007; 31(7):683-690. doi:10.1016/j.cellbi.2006.12.005
31.    Volk WA, Wheeler MF. Mikrobiologi dasar. Erlangga Jakarta. Published online 1993.
32.    Martínez‐Camacho AP, Cortez‐Rocha MO, Castillo‐Ortega MM, Burgos‐Hernández A, Ezquerra‐Brauer JM, Plascencia‐Jatomea M. Antimicrobial activity of chitosan nanofibers obtained by electrospinning. Polymer International. 2011; 60(12):1663-1669. doi:10.1002/pi.3174
33.    Suhartati R, Apriyani F, Virgianti DP, Fathurohman M. Antimicrobial activity test of Mangosteen leaves ethanol extract (Garcinia mangostana Linn) against Pseudomonas aeruginosa bacteria. In: Journal Of Physics: Conference Series. Vol 1179. IOP Publishing; 2019:12167. doi:10.22270/ajprd.v9i1.927
34.    Davis WW, Stout TR. Disc plate method of microbiological antibiotic assay: I. Factors influencing variability and error. Applied Microbiology. 1971; 22(4):659-665.
35.    Rakatama AS, Pramono A, Yulianti R. The antifungal inhibitory concentration effectiveness test from ethanol seed Arabica coffee (Coffea arabica) extract against the growth of Candida albicans patient isolate with in vitro method. In: Journal of Physics: Conference Series. Vol 970. IOP Publishing; 2018:12023. doi:10.1088/1742-6596/970/1/012023
36.    Zhang XF, Yang SL, Han YY, et al. Qualitative and quantitative analysis of triterpene saponins from tea seed pomace (Camellia oleifera abel) and their activities against bacteria and fungi. Molecules. 2014; 19(6):7568-7580. doi:10.3390/molecules19067568

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