Author(s):
Das Ashokkumar Nathulal, Rabinarayan Acharya, Preeti Pandya, Harisha CR, Shukla VJ
Email(s):
ashoklifecare2@gmail.com
DOI:
10.52711/0974-360X.2022.00649
Address:
Das Ashokkumar Nathulal1*, Rabinarayan Acharya2, Preeti Pandya3, Harisha CR4, Shukla VJ5
1PG Scholar, ITRA, Jamnagar 361008, Gujarat, India.
2Professor and Head, ITRA, Jamnagar 361008, Gujarat, India.
3M. Pharma and Ph. D, Department of Dravyaguna, ITRA, Jamnagar 361008, Gujarat, India.
4Head, Pharmacognosy, ITRA, Jamnagar 361008, Gujarat, India.
5Head, Pharmaceutical Laboratory, ITRA, Jamnagar 361008, Gujarat, India.
*Corresponding Author
Published In:
Volume - 15,
Issue - 9,
Year - 2022
ABSTRACT:
Background: Atalantia monophylla DC. (Syn. Limonia monophylla L.), family Rutaceae, root and stem have been reported for their use in rheumatism, arthritis, antidote to snake-bite, cough, allergies, skin diseases and as an antidote to snake-bite through ethnomedicinal claims. Present study reports a details pharmacognostic and preliminary physiochemical and phytochemical aspects of A. monophylla root and stem. Material and methods: The root and stem samples were collected after proper authentication from Athagarh forest Odisha India. Macroscopic observations were made with naked eyes, microscopy, powder microscopy, physicochemical and qualitative analysis and HPTLC studies of root and stem were carried out following Ayurvedic Pharmacopoeia of India recommended and others standard protocols. Results and Discussion: Root were 5-7cm in length; flattened, L-shaped, easily peel-able and thick, outer yellowish brown and inner yellow in colour with rootlets and scars. Stem cylindrical, striated, greenish brown in colour. Diagrammatic transverse section of root and stem shows circular in shape, outer cork, cortex followed by central stellar region and circular in nature, outer epidermis followed by hypodermis, cortex, vascular bundle and centrally located large pith respectively. Microscopic powder character of root and stem shows prismatic crystal, parenchyma cell with oil globule, simple starch grains, brown content and rhomboidal crystal, pitted parenchyma cells, brown content, oil globule, simple and compound starch grains respectively. Qualitative phytochemical analysis of root and stem shows the presence of carbohydrates, glycosides, saponins, alkaloids, tannins and flavonoids. HPTLC study of root and stem shows 22 and 25 spots at short (254nm) and 24 and 27 spots at long (366nm) ultraviolet respectively. Conclusion: The results obtained from pharmacognostical, phytochemical, physicochemical characteristic along with HPTLC studies are the diagnostic tool of A. monophylla plant and will help in standardization of its root and stem in terms of its quality, strength, purity.
Cite this article:
Das Ashokkumar Nathulal, Rabinarayan Acharya, Preeti Pandya, Harisha CR, Shukla VJ. Pharmacognostical and Preliminary Phytochemical Evaluation of Atalantia monophylla DC. root and stem. Research Journal of Pharmacy and Technology. 2022; 15(9):3871-8. doi: 10.52711/0974-360X.2022.00649
Cite(Electronic):
Das Ashokkumar Nathulal, Rabinarayan Acharya, Preeti Pandya, Harisha CR, Shukla VJ. Pharmacognostical and Preliminary Phytochemical Evaluation of Atalantia monophylla DC. root and stem. Research Journal of Pharmacy and Technology. 2022; 15(9):3871-8. doi: 10.52711/0974-360X.2022.00649 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2022-15-9-9
REFERENCES:
1. Saxena HO, Brahmam M. The Flora of Orrisa. Regional Research Laboratory and Orissa Forest Development Corporation Ltd., Printed at Capuial Business Service and Consultancy, Bhubaneshwar. April 1994; Vol. 1: pp.226.
2. Das AN, Acharya R. A promising ethnomedicinal plant Atalantia monophylla DC.: A Review. International Journal of Botany Studies. 2021 January 19 ;6(1):173-85. http://www.botanyjournals.com
3. Seow LJ, Beh HK, Sadikun A, Asmawi MZ. Preliminary phytochemical and physiochemical characterization of Gynura segetum (Lour) Merr (Compositae) Leaf, Tropical Journal of Pharmaceutical Research. 2013;12(5):777-782. http://dx.doi.org/10.4314/tjpr.v12i5.18
4. Saxena HO, Brahmam M. The Flora of Orrisa. Regional Research Laboratory and Orissa Forest Development Corporation Ltd., Printed at Capuial Business Service and Consultancy, Bhubaneshwar, April 1994: Vol.1: pp. 229-30.
5. Kirtikar KR, Basu BD. Indian Medicinal Plants, Printed at Jayyed Press, Delhi. 1975; Reprint ed, Vol. 1: pp. 482-83.
6. Evans WC. Trease and Evans Pharmacognosy, Published by Saunders Elsevier, London. 2009; 16th ed: pp. 567-568.
7. Khandelwal KR. Practical Pharmacognosy, Published by Nirali Prakashan Pune. 2008; 19th ed: pp. 9-19.
8. Anonymous. The Ayurvedic Pharmacopoeia of India. Government of India, Ministry of Health and Family Welfare, Department of AYUSH, Part-II, Vol-II; 1st ed: New Delhi. 2008.
9. Khandelwal KR. Practical Pharmacognosy, Published by Nirali Prakashan Pune. 2008; 19th ed: pp. 149-153.
10. Kabra A, Sharma R, Singla S, Kabra R, Baghel US. Pharmacognostic characterization of Myrica esculenta leaves. Journal of Ayurveda and Integrative Medicine. 2019 March 13; 10:18-24. http://elsevier.com/locate/jaim
11. Mariswamy Y, Gnaraj WE, Johnson M. Chromatographic finger print analysis of steroids in Aerva lanata L by HPTLC technique. Asian Pac J Trop Biomed. 2011 May 20;1(6):428-433. doi:10.1016/S2221-1691(11)60094-4
12. Priyanka Gupta, Darshana Patil, Avinash Patil. Qualitative HPTLC phytochemical profiling of Careya arborea Roxb. bark, leaves and seeds 3 Biotech. 2019 July 30; 9:311. doi: 10.1007/s13205-019-1846-x
13. Wagner H, Bladt S, Zgainski EM. Plant drug analysis, a thin layer chromatography atlas. Springer, Berlin, Heidenberg, New York. 1984.
14. Sasmal S, Majumdar S, Gupta M, Mukherjee A, Mukherjee PK. Pharmacognostical, phytochemical and pharmacological evaluation for the antipyretic effect of the seeds of Saraca asoca Roxb. Asian Pac J Trop Biomed. 2012 October;2(10):782-786. doi: 10.1016/S2221-1691(12)60229-9
15. Kanakiya A, Padalia H, Pande J, Chanda S. Physicochemical, phytochemical and Pharmacognostic study of Limnonium stocksii, a halophyte from Gujarat. The Journal of Phytopharmacology. 2018 April 26;7(3):312-318. http: //www.phytopharmajournal.com
16. Sharma V, Pracheta. Microscopic studies and preliminary pharmacognostical evaluation of Euphorbia neriifolia L. leaves. Indian Journal of Natural Products and Resources. 2013 February 11; 4 (4):348-357.
17. Netala S, Penmetsa R, Nakka S, Polisetty BL. Pharmacognostic study of Callistemon citrinus L. Bark. Int J Pharm Sci. 2015 November 25;7(1): 427-430.
18. Chanda S. Importance of pharmacognostic study of medicinal plants: An overview. Journal of Pharmacognosy and Phytochemistry. 2014 December 20;2 (5):69-73. https://www.phytojournal.com
19. Gupta PC, Rao V. Morpho-anatomical and physicochemical studies of Fumaria indica (Hausskn.) Pugsley. Asian Pac J Trop Biomed. 2012 October; 2(10):830-834. doi: 10.1016/S2221-1691(12)60238-X
20. Jia S, Shen M, Zhang F, Xie J. Recent Advances in Momordica charantia: Functional Components and Biological Activities. Int. J. Mol. Sci. 2017 November 28; 18:25-55 doi: 10.3390/ijms18122555
21. Yadav RNS, Agarwala M. Phytochemical analysis of some medicinal plants. Journal of Phytology. 2011 December 7: 3(12):10-14 http://journal-phytology.com/
22. Bo S, Hongzhu G, Yajun C, Min Y, Jian H, Dean G. Steroidal saponins from Smilax china and their anti-inflammatory activities. Phytochemistry. 2007 March;68(5):623-30. doi: 10.1016/j.phytochem.2006.10.026.
23. Renren B, Chuansheng Y, Zhichao Z, Jiamin G, Ziqiang B, Xiangyang Y, et al. Discovery of natural anti-inflammatory alkaloids: Potential leads for the drug discovery for the treatment of inflammation. European Journal of Medicinal Chemistry. 2021 March 5; 213:113-165. doi: 10.1016/j.ejmech.2021.113165
24. Arup KM, Sushil KV, Ranjeet K. Anti-inflammatory effect of an extract of Agave americana on Experimental Animal. Pharmacognosy Res. 2018 Jan-March;10(1):104-108. doi: 10.4103/pr.pr_64_17
25. Zhang Y, Wang K, Chen H, He R, Cai R, Li J, et al. Anti-inflammatory lignans and phenylethanoid glycosides from the root of Isodon ternifolius (D. Don) Kudo. Phytochemistry. 2018 May; 153:36-47. doi: 10.1016/j.phytochem.2018.05.017
26. Ambreen M, Mirza SA. Evaluation of anti-inflammatory and wound healing potential of tannins isolated from leaf callus cultures of Achyranthus aspera and Ocimum basilicum. Pak J Pharm Sci. 2020 January;33(1):361-69.