Author(s):
Lili Legiawati, Fadilah Fadilah, Kusmarinah Bramono, Aditya Indra Pratama
Email(s):
lililegiawati@yahoo.com
DOI:
10.52711/0974-360X.2023.00068 
Address:
Lili Legiawati1*, Fadilah Fadilah2, Kusmarinah Bramono1, Aditya Indra Pratama1
1Department of Dermatology and Venereology, Faculty of Medicine Universitas Indonesia, Dr.CiptoMangunkusumo National General Hospital, Jakarta, 10430, Indonesia.
2Chemistry Department in Medical Sciences, Faculty of Medicine Universitas Indonesia, 10430, Indonesia.
*Corresponding Author
Published In:
Volume - 16,
Issue - 1,
Year - 2023
ABSTRACT:
Superoxide dismutase (SOD) and glutathione peroxidase (GPx) are both parts of the enzymatic line in the antioxidant framework which changes anion superoxide to a more stable compound like oxygen (O2) and hydrogen peroxide (H2O2). Centella asiatica significantly shows antioxidant activity in several studies with comparable activity to ascorbic acid and butylated hydroxytoluene. This study assessed the antioxidant properties of Centella asiatica by studying its interaction with SOD and GPx. Active compounds of Centella asiatica were selected based on their interactions with SOD and GPx to determine which compounds reacted significantly. Significant interaction in the docking study was determined by the binding energy of each compound to the enzymes. Active compound of Centella asiatica had been proven to interact with both SOD and GPx. SOD bound with asiaticoside binding energy -10.2310 kcal/mol and madecassic acid binding energy -9.0518 kcal/mol. Based on protein residue, the majority of the protein bods into Gln 118. Both asiaticosside and madecassic acid bound to Gln118. Madasiatic acid and asiaticoside are bound to GPx with the lowest binding energy ligand, respectively -10.1232, -9.8082, and -8.5552 kcal/mol. Both madasiatic acid and asiaticoside had common binding residue of Arg189, Glu239, and Glu244.Our study conclude that the active compounds of Centella asiatica (asiaticoside, madecassic acid, and madasiatic acid) had proven to react significantly with SOD and GPx based on docking studies.
Cite this article:
Lili Legiawati, Fadilah Fadilah, Kusmarinah Bramono, Aditya Indra Pratama. In Silico Study of Centella asiatica Derivatives as Antioxidant: Enhancer of Superoxide Dismutase and Glutathione Peroxidase Activity. Research Journal of Pharmacy and Technology 2023; 16(1):399-3. doi: 10.52711/0974-360X.2023.00068
Cite(Electronic):
Lili Legiawati, Fadilah Fadilah, Kusmarinah Bramono, Aditya Indra Pratama. In Silico Study of Centella asiatica Derivatives as Antioxidant: Enhancer of Superoxide Dismutase and Glutathione Peroxidase Activity. Research Journal of Pharmacy and Technology 2023; 16(1):399-3. doi: 10.52711/0974-360X.2023.00068 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2023-16-1-68
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