Author(s): Chandni Tandon, Ruby George, Preeti Bajpai, Priti Mathur

Email(s): Email ID Not Available

DOI: 10.52711/0974-360X.2023.00734   

Address: Chandni Tandon1, Ruby George1, Preeti Bajpai2, Priti Mathur1* 1Amity Institute of Biotechnology, Amity University, Uttar Pradesh, Lucknow Campus - 226028, India. 2Zoology Department, Mahatma Gandhi Central University Motihari, Bihar, India. *Corresponding Author E-mail:

Published In:   Volume - 16,      Issue - 10,     Year - 2023

In view of urgent need for effective herbal antibiotics, we have isolated, characterized, an effective antibacterial compound Dinoxin B withanolide from leaves of Datura inoxia to investigate its antibacterial potential against Staphylococcus aureus. The bactericidal efficacy of Dinoxin B withanolide was assessed on Staphylococcus aureus, using transmission and scanning electron microscopy. Its effect on DNA and protein was analyzed by the electrophoresis technique. In vitro and in vivo studies were performed to evaluate the cytotoxicity and anti-inflammatory nature of the compound. Electron microscopic study showed that Dinoxin B withanolide damaged bacterial cell wall and membrane. It also causes degradation to DNA and protein, resulting in bacterial death. This compound was found to be non-toxic even at higher concentrations. Treatment of Balb/c mice revealed the significant suppression of T cells by the Dinoxin B withanolide. In vivo and in vitro, studies confirm that Dinoxin B withanolide could be used as herbal antibiotics with bactericidal, anti-inflammatory, low toxicity and good shelf life.

Cite this article:
Chandni Tandon, Ruby George, Preeti Bajpai, Priti Mathur. Mechanistic Studies of Dinoxin B withanolide, A Herbal Antibiotic from Datura inoxia against Staphylococcus aureus. Research Journal of Pharmacy and Technology 2023; 16(10):4504-1. doi: 10.52711/0974-360X.2023.00734

Chandni Tandon, Ruby George, Preeti Bajpai, Priti Mathur. Mechanistic Studies of Dinoxin B withanolide, A Herbal Antibiotic from Datura inoxia against Staphylococcus aureus. Research Journal of Pharmacy and Technology 2023; 16(10):4504-1. doi: 10.52711/0974-360X.2023.00734   Available on:

1.    Mariyappan M., Bharathidasan R., Mahalingam R., Madhanraj P., Panneerselvam A.. Antibacterial Activity of Cardiospermum halicacabum and Melothria heterophylla. Asian J. Pharm. Res. 2011; 1: 111–113 .
2.    Neeli Rose Ekka, Kamta Prasad Namdeo, P. K. S. Standardization Strategies for Herbal Drugs-An Overview. Res. J. Pharm. Tech. 2008; 1: 310–312.
3.    Sheetal V. Palande, D. K. S. Study of antimicrobial activity of 2-[(1-Naphthalen-1-yl-ethylimino)-methyl]-phenol and its transition metal complexes on E.coli and Staphylococcus aureus. Asian J. Res. Chem. 2018; 11: 19–22 . 10.5958/0974-4150.2018.00005.6.
4.    Tandon C, Mathur P, Sen M, Kanojiya S. Identification of an antibacterial withanolide (Dinoxin b) from leaf of datura inoxia mill. Int J. Phytomedicine. 2016; 8:1–12.
5.    Vermillion K, Holguin FO, Berhow MA, Richins RD, Redhouse T, O’Connell MA, et al. Dinoxin B, a withanolide from Datura inoxia leaves with specific cytotoxic activities. J Nat Prod. 2011; 74:267–
6.    Kaatz GW, McAleese F, Seo SM. Multidrug resistance in Staphylococcus aureus due to overexpression of a novel multidrug and toxin extrusion (MATE) transport protein. Antimicrob Agents Chemother. 2005; 49:1857–64.
7.    Gurusamy KS, Koti R, Toon CD, Wilson P, Davidson BR. Antibiotic therapy for the treatment of methicillin-resistant Staphylococcus aureus (MRSA) infections in surgical wounds. Cochrane Database Syst Rev. 2013;.
8.    Wissam Zam, Rim Harfouch, Al Dwiri Mais KR. Anti-Staphylococcus aureus efficacy of six natural honey samples originated from Syria. Res J Pharmacogn Phytochem. 2018; 10:23–6.
9.    S. Mohammed Shahid NU. Spectrum of Antimicrobial Susceptibility of E. coli and Staphylococcus aureus Isolates from Clinical Samples. Res J Pharm Tech. 2015; 8:1399–402.
10.    Shrivastava A, Jain R, Agrawal R, Ahirwar D. Clinical Trial of Herbal Drugs and Products in India: Past and Current Status and Critical Issues. Res J Pharm Technol. 2008;1:69–74.
11.    Tandon C, Mathur P, Sen M. Inhibitory activity of crude and purified extracts of datura inoxia against some clinically isolated bacteria. Res J Pharm Technol. 2018;11:695–9.
12.    Oussama Mansour, Rim Salamma LA . Screening of Antibacterial activity in Vitro of Cyclamen hederifolium tubers Extracts. Res J Pharm Tech. 2016; 9:1837–1839.
13.    Jeyanthi T, Subramanian P, Kumaravel P. A comparative analysis of antibacterial activity of Withania somnifera root extract with commercial antibiotics. Asian J Pharm Res. 2013; 3:98–102.
14.    Imura Y, Nishida M, Matsuzaki K. Action mechanism of PEGylated magainin 2 analogue peptide. Biochim Biophys Acta – Biomembr. 2007;1768:2578–85.
15.    Nishiguchi MK, Doukakis P, Egan M, Kizirian D, Phillips A, Prendini L, et al. DNA Isolation Procedures. Methods Tools Biosci Med. 2002:250–81.
16.    Sánchez I, Seseña S, Palop L. Identification of lactic acid bacteria from spontaneous fermentation of “Almagro” eggplants by SDS-PAGE whole cell protein fingerprinting. Int J Food Microbiol. 2003;82:181–189.
17.    Kaushik P, Goyal P. In vitro evaluation of Datura innoxia (thorn-apple) for potential antibacterial activity. Indian J Microbiol. 2008;48:353–7.
18.    Milovanova TN, Popma SH, Cherian S, Moore JS, Rossman MD. Flow cytometric test for beryllium sensitivity. Cytom Part B - Clin Cytom. 2004;60:23–
19.    Mao Z, Liu Z, Chen L, Yang J, Zhao B, Jung YM, et al. Predictive value of the surface-enhanced resonance Raman scattering-based MTT assay: A rapid and ultrasensitive method for cell viability in situ. Anal Chem. 2013;85:7361–8.
20.    Singh M, Prathapan A, Nagori K, Ishwarya S, Raghu K. Cytotoxic and Antimicrobial Activity of Methanolic Extract of Boerhaavia diffusa L. Res J Pharm Technol. 2010;3:1061–1063.
21.    Murphy BS, Sundareshan V, Cory TJ, Hayes D, Anstead MI, Feola DJ. Azithromycin alters macrophage phenotype. J Antimicrob Chemother. 2008; 6(1): 554–60.
22.    Xu JG, Liu T, Hu QP, Cao XM. Chemical composition, antibacterial properties and mechanism of action of essential oil from clove buds against staphylococcus aureus. Molecules. 2016; 21.
23.    George R, Mathur P, Tandon C. Dinoxin B Withanolide from Datura inoxia Mill as an Effective Phytocompound Against Urinary Tract Infection causing Staphylococcus aureus. 2021;14:1707–
24.    Tan J, Liu Y, Cheng Y, Sun Y, Pan J, Guan W, et al. New withanolides with anti-inflammatory activity from the leaves of Datura metel L. Bioorg Chem. 2020;95.
25.    Susmi M S, Revathy S Kumar, Sreelakshmi V, Sruthy V Menon, Surya Mohan, Saranya Tulasidharan Suja, Sathianarayanan AAM. A Computational approach for identification of Phytochemicals for targeting and optimizing the inhibitors of Heat shock proteins. Res J Pharm Tech. 2015;8:1199-1204. 10.5958/0974-360X.2015.00219.X.
26.    Baig MW, Nasir B, Waseem D, Majid M, Khan MZI, Haq I ul. Withametelin: a biologically active withanolide in cancer, inflammation, pain and depression. Saudi Pharm J. 2020; 28:1526–
27.    Radhika Chelamalla AM. Molecular docking studies and ADMET Predictions of Pyrimidine Coumarin Scaffolds as Potential IDO Inhibitors. Asian J Res Chem. 2017;10:331–340.

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