Author(s): El Aboubi M, Bikri S, Hsaini A, Berrid N, Aouane M


DOI: 10.52711/0974-360X.2023.00794   

Address: El Aboubi M1, Bikri S2*, Hsaini A2, Berrid N1, Aouane M1
1Laboratory of Natural Resources and Sustainable Development, Biology Department, Ibn Tofail University, Faculty of Sciences, Kenitra, Morocco.
2Laboratory of Biology and Health, Biology Department, Ibn Tofail University, Faculty of Sciences, Kenitra, Morocco.
*Corresponding Author

Published In:   Volume - 16,      Issue - 10,     Year - 2023

The objective of this study is to investigate the effects of Moroccan lemon peel essential oil (LPEO) on various aspects such as anti-hyperglycemic, anti-inflammatory, antioxidant, and anxiolytic activities in diabetic rats. The animals were divided into five groups: normal control group, diabetic control group, diabetic group treated with metformin (300mg/kg), and two diabetic groups treated with LPEO at doses of 0.6ml/kg and 1.2ml/kg, respectively. T2DM was induced in the rats by administering intraperitoneal injections of Streptozotocin and Nicotinamide. Treatment with LPEO or metformin was initiated immediately after confirming the presence of hyperglycemia and continued for a duration of four weeks. Anxiety-related behavior in the rats was assessed using two behavioral tests. 24 hours after conducting the behavioral tests, all rats were anesthetized, and blood samples were taken for the analysis of lipid parameters. The pancreas and brain were dissected to assess TNF-alpha, SOD, and MDA levels. Treatments with LPEO contributed significantly to the preservation of lipid profile and reduction of the atherosclerosis index in diabetic rats (p<.05). Additionally, there was a notable decrease in MDA and TNF-a concentration in the pancreas and brain of diabetic rats treated with LPEO associated with a remarkable increase in SOD activity in the pancreas and brain compared to diabetic control group (p<.05). Lastly, both LPEO and metformin treatments demonstrated significant improvements in anxiety-related behavior in this rat model (p<.05). These positive effects could potentially be attributed to the potent antioxidant and anti-inflammatory activities of both treatments.

Cite this article:
El Aboubi M, Bikri S, Hsaini A, Berrid N, Aouane M. The protective effect of Lemon Peel Essential Oil on Pancreas and Brain Oxidative Stress and Inflammation induced by Streptozotocin-Nicotinamide in rats: Behavioral and Biochemical Evidences. Research Journal of Pharmacy and Technology 2023; 16(10):4897-6. doi: 10.52711/0974-360X.2023.00794

El Aboubi M, Bikri S, Hsaini A, Berrid N, Aouane M. The protective effect of Lemon Peel Essential Oil on Pancreas and Brain Oxidative Stress and Inflammation induced by Streptozotocin-Nicotinamide in rats: Behavioral and Biochemical Evidences. Research Journal of Pharmacy and Technology 2023; 16(10):4897-6. doi: 10.52711/0974-360X.2023.00794   Available on:

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