Author(s): Moushumi Baidya, Shvetank Bhatt, Himangshu Sekhar Maji, Kuntal Manna, J. Anbu


DOI: 10.52711/0974-360X.2023.00918   

Address: Moushumi Baidya1,2*, Shvetank Bhatt3, Himangshu Sekhar Maji1, Kuntal Manna4, J. Anbu5
1Department of Pharmaceutical Technology, JIS University, Kolkata, West Bengal, India - 700109.
2Bharat Pharmaceutical Technology, Agartala, West Tripura, 799130, India.
3School of Health Sciences and Technology, Dr. Vishwanath Karad MIT World Peace University, Pune - 411038, India.
4Natural Cum Advance Synthetic Lab, Department of Pharmacy, Tripura University (A Central University), Suryamaninagar - 799022, India.
5Department of Pharmacology, Faculty of Pharmacy, M.S. Ramaiah University of Applied Sciences, MSR Nagar, Bangalore - 560054, India.
*Corresponding Author

Published In:   Volume - 16,      Issue - 12,     Year - 2023

The purpose of the present study is to evaluate the antitumor activity (ATA) of traditional herbal preparation Sivanar Amirtham (SA) on Dalton’s Lymphoma Ascites. Siddha medicine system (SMS) is a traditional system of medicine originated from ancient Tamilakam of South India. Siddha medicine is a traditional healing system from Tamilakam in ancient South India. For our purpose, we have performed acute toxicity (AT) study as per OECD guidelines 423 and ATA by xenograft method. In this study, a single dose of 300, 1000 and 2000 mg/kg of Sivanar Amirtham suspension (SAS) was orally (p.o.) administered in mice and animals were observed for 14 days. For antitumor study (ATS), we have used DAL cells which were intraperitoneally (i.p.) inoculateded into mouse. The ATAs were studied by monitoring the parameters such as cell growth inhibitors, tumor weight measurements, mean survival time of DAL bearing mice as well as changes in depleted haematological and biochemical parameters due to tumorigenesis. The SAS was also evaluated for in vitro cytotoxicity study in different concentration and the viability of cells was determined by exclusion method of trypan blue dye (TBD). The AT study showed no signs of toxicity and no mortality after single administration of SAS. SAS caused significant decrease in packed cell volume (PCV) (value), Tm volume (value) and viable cell count (value), and it prolonged the life span of DAL Tm carrying mice. Haematological and biochemical profiles were reverted to normal levels in SAS treated mice. The results of in vitro cytotoxicity show that SAS showed significant ATA in mice with moderate DAL levels. The IC50 value turned into discovered to be 800 µg/ml from the in vitro cytotoxicity examine. The study strongly suggests that SAS has the potential to be an antitumor medication against DAL cells induced Tm and it can be extrapolated for further cancer (CA) prevention applications.

Cite this article:
Moushumi Baidya, Shvetank Bhatt, Himangshu Sekhar Maji, Kuntal Manna, J. Anbu. Evaluation of Antitumor Activity of Sivanar Amirtham – A Herbomineral Formulation belonging to Indian Traditional System of Medicine using Dalton's Ascites Lymphoma in Mice. Research Journal of Pharmacy and Technology. 2023; 16(12):5677-3. doi: 10.52711/0974-360X.2023.00918

Moushumi Baidya, Shvetank Bhatt, Himangshu Sekhar Maji, Kuntal Manna, J. Anbu. Evaluation of Antitumor Activity of Sivanar Amirtham – A Herbomineral Formulation belonging to Indian Traditional System of Medicine using Dalton's Ascites Lymphoma in Mice. Research Journal of Pharmacy and Technology. 2023; 16(12):5677-3. doi: 10.52711/0974-360X.2023.00918   Available on:

1.    Armour FE, Blood FR, Belden DA. The manual for laboratory work in mammalian Physiology. The University of Chicago Press Chicago. 1965; 3: 4-6.
2.    Alkhatib MH, Alshehri WS, Abdu FB. In vivo evaluation of the anticancer activity of the gemcitabine and doxorubicin combined in a nanoemulsion. J Pharm Bioall Sci. 2018; 10(1): 35-42. doi:
3.    Baidya M, Anbu J, Akhtar MS, Sarkar S, Mandal SK. Antimicrobial Evaluation of Ethanolic Extract of Selected Seed Shells. Int J Curr Pharma Res. 2020; 12(6): 74-76. doi:
4.    Bala A, Biswakanth K, Haldar PK, Mazumder UK, Bera S. Evaluation of anticancer activity of Cleome gynandra on Ehrlich’s Ascites Carcinoma treated mice, J. Ethnopharmcol. 2010; 129(1): 131-134. doi:
5.    Coussens L, Werb Z. Inflammation and cancer. Nature. 2002; 420: 860–867. doi:
6.    Mandal SK, Biswas D, Sony A, De A, Dastider D, Baidya M, Paul S, Dawn S, Mandal S, Plants and phytochemicals in the treatment of colorectal Cancer: recent updates, Int. J. Biol. Pharm. Allied Sci. 2021; 10(10): 3502-3521. doi:org/10.31032/IJBPAS/2021/10.10.5657.
7.    Clarkson BD, Burchenal JH. Preliminary screening of antineoplastic drugs. Prog. Clin. Cancer. 1965; 1: 625-629.
8.    Dacie JV, Lewis SM. Practical Haematology. (12th edition)  Elsevier, 2017; doi:
9.    Dahab RA, Afifi F. Antiproliferative activity of selected medicinal plants of Jordan against a breast adenocarcinoma cell lines (MCF7). Sci Pharm Inc. 2007; 75(3): 121-136. doi:
10.    Farah AO, Nooraain H, Noriham A, Azizah AH, Nurul-Husna R. Acute and oral subacute toxicity study of ethanolic extract of Cosmos caudatus leaf in Sprague Dawley rats. Int J Biosci Biochem Bioinforma. 2013; 3(4): 301–305. doi:
11.    Graham JG, Quinn M.L, Fabricant DS, Farnsworth NR. Plants used against cancer-an extension of the work of Jonathan Hartwell. Journal of Ethnopharmacol. 2000; 73: 347-377. doi:
12.    Ganapathy P, Elumalai K, Arumugam MK, Amulya CS, Manivel R. Anticancer potential of Siddha formulations against oral cancer cell line in vitro. Trends Med. 2019; 19: 1-6. doi:
13. Accessed on 06/12/2021.
14. › 2019/11/25 › sivanar-amirtham. Accessed on 06/12/21.
15.    Hogland HC. Hematological complications of cancer chemotherapy. Semin Oncol . 1982; 9: 95-102.
16.    Muthu IS, Kanth SB, K. Kalimuthu K, Sangiliyandi G. Antitumor activity of silver nano particles in Dalton’s lymphoma ascites tumor mode.  Int. J. of Nano Med. 2010; 5: 753-762. doi:
17.    Baidya M, Jayaraman A,  Maji HS, Ramya Krishna PS, Das D, Evaluation of acute and sub-acute toxicity of sivanar amirtham in albino mice and wistar albino rats, J. Med. P’ceutical Allied Sci. 2022; 11(1): 4196 - 4204. doi: 10.22270/jmpas. V11I1.2217.
18.    Venu Gopal Y, Ravindranath A, Kalpana G, Rajkapoor B, Sreenivas SA . Antitumor and antioxidant activity of Diospyros peregrina against Dalton’s ascites lymphoma in rodents. Annals of Biological Research. 2012; 3 (11):4985-4992.
19.    Ramya Krishna PS, Jayaraman A, Baidya M, Nayak DA.Toxicological evaluation of fucoidan, a polysaccharide isolated from Turbinaria conoides (J. agardh) Kutzing procured from mandapam coastal area, tamilnadu, J. Med. P’ceutical Allied Sci. 2022; 11(2): 4629 – 4635. doi: 10.55522/jmpas.V11I2.2511.
20.    Gupta M. Antitumor Activity and Antioxidant Status of Caesalpinia bonducella against Ehrlich Ascites Carcinoma in swiss albino mice. J. Pharmacol. Sci. 2004; 94(2): 177-184. doi:
21.    Price VE, Greenfield RE. Anemia in cancer. Adv. Can. Res. 1958; 5: 199-200. doi:  
22.    Babu P, Anbu J, Ashwini A, Velpandian V. Anti tumour activity of guru pathangam against dalton ascites lymphoma (dal) in mice. Int J Pharm Bio Sci. 2012; 3(3): 281 – 289.
23.    Meera R, Chidambaranathan N. Anti cancer Activity of Ethanolic Extract of Crataeva magna Lour (DC) against Ehrlich Ascitic Carcinoma Cell Lines in Mice. J. Pharm. Sci. and Res. 2017; 9(10): 1869-1873.
24.    Baidya M, Maji HS, Bhatt S, Das D, In-vitro Evaluation of the Thrombolytic and Anti-inflammatory Activity of Capparis sepiaria Root Extracts, J. Med. P’ceutical Allied Sci. 2022; 11(1), 4166-4171. doi: 10.22270/jmpas.V11I1.1879.
25.    Rajesh kumar NV, Kuttan G, Ramsewak RS, Nair MG, Kuttan R. Antitumour and anticarcinogenic activity of Phyllanthus amarus extract. J. Ethnopharmacol.2002; 81(1): 17-22. doi: 10.1016/s0378-8741(01)00419-6.
26.    Byahatti S, Bogar C, Bhat K, Dandagi G.Evaluation of anticancer activity of Melaleuca alternifolia. (i. e. tea tree oil) on Leukemia cancer cell line (K562): An in vitro study. J. Med. Plants Stud. 2018; 6(5): 01-06.
27.    Sheeja KR, Kuttan G, Kuttan R. Cytotoxic and antitumour activity of Berberin. Amala Res. Bull.1997; 17:73-76.
28.    Sur P, Ganguly DK. Tea plant roots extract (TRE) as an antineoplastic agent. Planta Med. 1994; l60: 106-109. doi:
29.    Yadavalli V.Evaluation of anticancer compounds from suspension cultures of Holy Basil (Ocimum sanctum L.) Int. J. Green Pharm. 2019; 13 (2): 98.
30.    Mandal SK, dawn S, Mondal S, Sarkar S, Mandal R, Baidya M, Paul S, Pramanick A, Dastider D, Sen DJ, Das A, Kolay A, The therapeutic versatility of quinolines, Int. J. Biol. Pharm. Allied Sci. 2021; 10(7): 2161-2181. doi:org/10.31032/IJBPAS/2021/10.7.5528.
31.    Wintrobe MM, Lee GR, Boggs DR. Clinical Hematology .1961; 5, Philadelphia.
32.    Sanakousar K. Patel, Arun K Shutter, Ria Patil, Archana Desangi, Vishalakshi Malali, Jyoti Patil, Sumangala Patil, Kusal K. Das, Prachi P. Parvatikar. In-Vitro Antioxidant, Anti-Inflammatory and Cytotoxic effects of different Solvent Extraction Terminalia chebula, Terminalia billerica, Phyllanthus emblica. Research Journal of Pharmacy and Technology. 2022; 15(7): 2940-4. doi: 10.52711/0974-360X.2022.00490.
33.    A. Mohamed Niyaz, S. Ravikumar. Antilarval and in vitro Anticancer efficacy of Cladode extracts of Opuntia dillenii (Ker Gawl.) Haw., Cereus pterogonus Lem. and Acanthocereus tetragonus (L.) Hummelinck. Research Journal of Pharmacy and Technology. 2022; 15(7): 2877-2. doi: 10.52711/0974-360X.2022.00480.
34.    J. Mariya Jothi, A. Jayaprakash. Phytochemical Analysis and In Vitro Anticancer Activity of Enteromorpha compressa against Ovarian Cancer.Research Journal of Pharmacy and Technology. 2022; 15(5): 1943-7. doi: 10.52711/0974-360X.2022.00323
35.    Yogita S. Ozarde, Vishnu P. Choudhari. Preliminary Qualitative Phytochemical Analysis and Acute Oral Toxicity Study of Latex of an Ethnomedicinal Plant Euphorbia fusiformis Buch.-Ham.Ex D.Don. Research Journal of Pharmacy and Technology. 2022; 15(3): 1123-7. doi: 10.52711/0974-360X.2022.00188.
36.    Hemant Devidas Une, Lalita Bansidas Bhagure. The Anti-leukemic Potential of Cyclea peltata as Validated by Phytochemical and Cell Line Studies. Research Journal of Pharmacy and Technology. 2022; 15(3): 1064-0. doi: 10.52711/0974-360X.2022.00178.
37.    Amaq Fadholly, Arif N. M. Ansori, Budi Utomo. Anticancer Effect of Naringin on Human Colon Cancer (WiDr Cells): In Vitro Study. Research Journal of Pharmacy and Technology. 2022; 15(2): 885-8. doi: 10.52711/0974-360X.2022.00148.
38.    Fitriyanti Jumaetri Sami, Nunuk Hariani Soekamto, Tatsufumi Okino, Firdaus, Jalifah latip. A Flavonoid compound of Turbinaria decurrens Bory with The Potential Antioxidant and Anticancer Activity. Research Journal of Pharmacy and Technology. 2021; 14(12): 6207-0. doi: 10.52711/0974-360X.2021.01074.
39.    Asish Bhaumik, Samaresh Datta, Susmita Datta, Radheshyam Samanta, B. D. Tripathi. Extraction and Isolation of Phenolic compounds from Sweet lime and Evaluation of Anticancer potentiality followed by Molecular docking against Topoisomerase II. Research Journal of Pharmacy and Technology. 2021; 14(11): 5993-7. doi: 10.52711/0974-360X.2021.01041.
40.    Raghad J. Fayyad, Alaa Naseer Mohammed Ali, Noor T. Hamdan. The Specific Anti-cancerous Mechanisms Suggesting Spirulina Alga as a Promising breast Cancer Fighter. Research Journal of Pharmacy and Technology 2021; 14(10): 5599-2. doi: 10.52711/0974-360X.2021.00974
41.    Syafruddin Ilyas, Rostime H. Simanullang, Salomo Hutahaean, Rosidah Rosidah, Putri C. Situmorang. Effect of Zanthoxylum acanthopodium methanol extract on CDK4 expression to cervical cancer. Research Journal of Pharmacy and Technology. 2021; 14(11): 5647-2. doi: 10.52711/0974-360X.2021.00982.

Recomonded Articles:

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

56th percentile
Powered by  Scopus

SCImago Journal & Country Rank

Recent Articles


Not Available