Author(s): M. Sivakumar, V.S. Chandrasekaran, B. Pushpa Kumari


DOI: 10.52711/0974-360X.2023.00106   

Address: M. Sivakumar1, V.S. Chandrasekaran2*, B. Pushpa Kumari3
1Professor, HOD, Department of Pharmacognosy, Faculty of Pharmacy, Sree Balaji Medical College and Hospital, Bharat Institute of Science and Technology (DU), Chennai.
2Associate Professor, Department of Pharmaceutical Biotechnology, Krishna Teja Pharmacy College, Tirupati 517506.
3Professor, Department of Pharmacology, Krishna Teja Pharmacy College, Tirupati.
*Corresponding Author

Published In:   Volume - 16,      Issue - 2,     Year - 2023

Rheumatoid arthritis (RA) is a systemic, chronic inflammatory and auto immune disorder. RA is provoked by genetical predisposition and environmental factors. Oxidative stress makes the human liable to diseases like diabetes, RA, cancer, atherosclerosis etc. Hence the study investigated the anti-arthritic and oxidative activity of ethanolic extract of Glycosmis pentaphylla (EEGP) as it has not been pharmacologically tested. Anti-arthritic potential was evaluated in vitro using protein denaturation by bovine serum albumin and in vivo by Complete Freund Adjuvant (CFA) models at 200 and 400mg/kg doses. Also, in vitro and in vivo antioxidant ability was appraised by DPPH scavenging and assessment of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and lipid peroxidation (LPO). The results exhibited concentration dependent inhibition of albumin denaturation with maximum results obtained at 800µg/ml. The results of CFA model depicted better protection against arthritic lesions and body weight alterations. Also G. pentaphylla remarkably ameliorated histopathological changes. Additionally, plant exhibited remarkable anti-oxidant activity.

Cite this article:
M. Sivakumar, V.S. Chandrasekaran, B. Pushpa Kumari. In vitro and in vivo Anti-arthritic and Antioxidant potential of Glycosmis pentaphylla in Complete Freund’s Adjuvant model. Research Journal of Pharmacy and Technology 2023; 16(2):621-6. doi: 10.52711/0974-360X.2023.00106

M. Sivakumar, V.S. Chandrasekaran, B. Pushpa Kumari. In vitro and in vivo Anti-arthritic and Antioxidant potential of Glycosmis pentaphylla in Complete Freund’s Adjuvant model. Research Journal of Pharmacy and Technology 2023; 16(2):621-6. doi: 10.52711/0974-360X.2023.00106   Available on:

1.    Ariful, H.M et al Ethnomedicinal uses of some medicinal plants for prevention against all forms of cancer by the traditional healers in Gazipur district of Bangladesh. AACR International conference on frontiers in cancer prevention research. Cancer Prev Res. 2010;Vol 3.  DOI: 10.1155/2016/7832120.
2.    Anonymous, In., The Wealth of India A dictionary of Indian Raw materials and Industrial Products Products Publication & Information Directorate, Vol. IV, CSIR, New Delhi. 2003; PMCID: PMC5189551.
3.    Shyama S. Kumar et al  Indian Medicinal Plants Used for Treatment of Rheumatoid Arthritis, Research J. Pharm. and Tech.2015; 8(5), 597-610. DOI: 10.5958/0974-360X.2015.000992
4.    Srivastava S, Patel et al  Rheumatoid Arthritis: An Autoimmune Disease Prevalent in Females. Research J. Pharm. and Tech. 2016; 9(2), 170-172. DOI: 10.5958/0974-360X.2016.000305
5.    Nandgude Tanaji et al Clinical features and treatment of Rheumatoid Arthritis; A review. Research J. Pharm. and Tech.2018; 11(12), 5701-5706. DOI: 10.5958/0974-360X.2018.010326.
6.    Bhattacharyya, P, et al Glycozolidol, an antibacterial carbazole alkaloid from Glycosmis pentaphylla.Phytochemistry.1985; 24(4), 882-883. doi:10.3390/molecules24020293
7.    Namita, A, et al Pharmacognostic and Preliminary phytochemical studies on the leaves of Glycosmis pentaphylla (Retz.) Corr. J. pharm. Res. 2011;4: 1800-1801. DOI: 10.13040/IJPSR.0975-8232.12(2).1135-42.
8.    McCartney-Francis, N., et al Suppression of arthritis by an inhibitor of nitric oxide synthase. The Journal  of  experimental  medicine, 1983;178(2), 749-754. doi: 10.1084/jem.178.2.749.
9.    McCartney-Francis, et al Inflammatory Joint Disease. In Inflammation Protocols (pp. 147-159). Humana Press.2013; doi: 10.4314/ajtcam.v9i1.2
10.    Praveen D et al  Antioxidant and Analgesic Activity of Leaf Extracts of Artocarpus heterophyllus. Research J. Pharm. and Tech. 2016;9(3); 257-261. doi: 0.5958/0974-360X.2016.00047.0.
11.    B. Premkuma Antioxidant Defense and Disease activity in Rheumatoid Arthritis. Research J. Pharm. and Tech. 2018;11(5):1810-1814. doi: 10.5958/0974-360X.2018.00336.0.
12.    Pavithra. S, N. Banu Free Radical Scavenging Activity and Total Antioxidant Capacity of Tin Chlorophyllin from Morinda citrifolia L. Research J. Pharm. and Tech.2017; 10(2): 453-455. doi: 10.5958/0974-360X.2017.00091.9.
13.    Sripradha and lakshmi T In vitro Anti arthritic activity of Sesbania grandiflora ethyl acetate extract. Research J. Pharm. and Tech.2015; 8(11); 1509-1511. DOI: 10.5958/0974-360X.2015.00269.3.
14.    S.B. Prabha, et al Evaluation of in vitro Antioxidant, Antibacterial and Anticancer activities of leaf extracts of Cleome rutidosperma. Research J. Pharm. and Tech.2017; 10(8): 2492-2496. doi: 10.5958/0974-360X.2017.00440.1.
15.    V. Chitra, J. Narayanan. In vitro Screening for Anti-Cholinesterase and Anti Oxidant Activity of Extract of Garcinia hanburyi. Research J. Pharm. and Tech 2018; 11(7): 2918-2921. doi: 10.5958/0974-360X.2018.00538.3.
16.    Clatworthy, A. L, et al. Role of peri-axonal inflammation in the development of thermal hyperalgesia and guarding behavior in a  rat  model  of  neuropathic  pain. Neuroscience letters,1985;184(1), 5-8. DOI: 10.1016/0304-3940(94)11154-b .
17.    David. B.L, et al.  Anti-inflammatory effects of ABT-702, a novel non-nucleoside adenosine kinase inhibitor, in rat adjuvant arthritis.Journal of Pharmacology and Experimental Therapeutics, 2001;296(2), 495-500. PMID: 11160636.
18.    Anderson, G. D, et al.  Selective inhibition of cyclooxygenase (COX)-2 reverses inflammation and expression of COX-2 and interleukin 6 in  rat  adjuvant  arthritis. Journal  of  Clinical Investigation, 1996;97(11), 2672-2679. doi: 10.1172/JCI118717.
19.    Fereidoni, M, et al. An accurate and simple method for measurement of paw edema. Journal of Pharmacological and Toxicological Methods,2000; 43(1), 11-14. DOI: 10.1016/s1056-8719(00)00089-7.
20.    Gutteridge, J. M.  Antioxidant properties of the proteins caeruloplasmin, albumin and transferrin. A study of their activity inserum   and   synovial   fluid   from   patients   with    rheumatoid arthritis. Biochimica et Biophysica Acta (BBA)-Protein Structure and Molecular Enzymology, 1986;869(2), 119-127. DOI: 10.1016/0167-4838(86)90286-4 .
21.    Kannan, S ―Free radical theory of autoimmunity‖. Theror Biol Med Model, 2006;3: 22. doi: 10.1186/1742-4682-3-22.
22.    Alamgeer* , Ambreen Malik Uttra and Umme Habiba Hasan  Anti-arthritic activity of aqueous-methanolic extract and various fractions of Berberis orthobotrys Bien ex Aitch. BMC Complementary and Alternative Medicine .2017; 17:371 DOI 10.1186/s12906-017-1879-9.
23.    Patil MVK, Kandhare AD, Bhise Anti-arthritic and anti-inflammatory activity of Xanthium srtumarium L. ethanolic extract in Freund’s complete adjuvant induced arthritis. Biomed Aging Pathol.;2012;2:6–15 DOI: 10.1016/j.jep.2014.05.023.
24.    Philippe, L et al.  Relations between functional, inflammatory, and degenerative parameters during adjuvant arthritis in rats. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 1997;273(4), R1550-R1556.

Recomonded Articles:

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

56th percentile
Powered by  Scopus

SCImago Journal & Country Rank

Recent Articles


Not Available