Author(s):
Rachagolla Sai Prathap Yadav, Belle Vijetha Shenoy, Nitish Kumar, G Prasanna Kumar, S Naveen Kumar
Email(s):
vijetha.shenoy@manipal.edu
DOI:
10.52711/0974-360X.2023.00178 
Address:
Rachagolla Sai Prathap Yadav1, Belle Vijetha Shenoy2*, Nitish Kumar3, G Prasanna Kumar4, S Naveen Kumar5
1, 2Department of Biochemistry, Kasturba Medical College, Manipal, Manipal Academy of Higher Education, Manipal, Karnataka, India – 576104.
3Department of Pharmacology, Manipal College of Pharmaceutical Science, Manipal Academy of Higher Education, Manipal, Karnataka, India - 576104.
3Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research, Hajipur, Export Promotions Industrial Park (EPIP), Industrial Area, Hajipur, Vaishali 844102, Bihar, India.
4Department of Biotechnology, Manipal College of Pharmaceutical Sciences, Manipal, Manipal Academy of Higher Education, Manipal, Karnataka, India - 576104.
5Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal, Manipal Academy of Higher Education, Manipal, Karnataka, India - 576104.
*Corresponding Author
Published In:
Volume - 16,
Issue - 3,
Year - 2023
ABSTRACT:
The pathophysiology of Alzheimer's disease (AD) typically begins before symptoms manifest. Early diagnosis and treatment can slow down the disease progression and improve the prognosis. Decreased acetylcholine, antioxidants, and polyunsaturated fatty acids enhance the progression of AD. Phytochemicals present in the Cucurbita pepo (C. pepo) plants may have anti-cholinesterase activity. Therefore, this study was performed using ethanolic extract of pumpkin seeds in the AlCl3 induced AD in Sprague Dawley rats. These rats were categorized into five groups, i.e., control, disease control (AlCl3), standard (Rivastigmine), and two treatment groups, one with 100mg/kg and the other with 200mg/kg of the pumpkin seed extract which was given once daily orally for 28days. The rats were assessed for behavioral and biochemical parameters such as antioxidant enzymes and acetyl-cholinesterase levels in brain homogenate. There was statistically significant (p<0.005) improvement in behavioral parameters and increase antioxidant levels and decrease acetylcholinesterase levels in the seed extract treatment groups compared to the AD rats. GC-MS analysis of C.pepo seeds showed the presence of polyunsaturated fatty acids, which have a significant role in neurite outgrowth activity. To conclude, the 80% ethanolic extract of Cucurbita pepo (pumpkin seeds) has anticholinesterase, antioxidant, and anti-inflammatory activity and can prevent memory impairment.
Cite this article:
Rachagolla Sai Prathap Yadav, Belle Vijetha Shenoy, Nitish Kumar, G Prasanna Kumar, S Naveen Kumar. In vivo Acetylcholinesterase activity and Antioxidant property of Cucurbita pepo ethanolic extract in Alzheimer’s disease induced by Aluminium chloride in Sprague Dawley rat model. Research Journal of Pharmacy and Technology 2023; 16(3):1065-1. doi: 10.52711/0974-360X.2023.00178
Cite(Electronic):
Rachagolla Sai Prathap Yadav, Belle Vijetha Shenoy, Nitish Kumar, G Prasanna Kumar, S Naveen Kumar. In vivo Acetylcholinesterase activity and Antioxidant property of Cucurbita pepo ethanolic extract in Alzheimer’s disease induced by Aluminium chloride in Sprague Dawley rat model. Research Journal of Pharmacy and Technology 2023; 16(3):1065-1. doi: 10.52711/0974-360X.2023.00178 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2023-16-3-14
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