Author(s):
Sumit Kumar, Rekha D Kini, Nayanatara Arun Kumar, Megha Gokul, Vivek Pai M, Shyamala Nayak
Email(s):
rekha.kini@manipal.edu
DOI:
10.52711/0974-360X.2023.00352 
Address:
Sumit Kumar1, Rekha D Kini2, Nayanatara Arun Kumar2, Megha Gokul2, Vivek Pai M3, Shyamala Nayak4
1Sumit Kumar, Tutor, Department of Physiology, KVG Medical College and Hospital, Sullia University, Deralakatte, Mangalore.
2Department of Physiology, Kasturba Medical College, Mangalore, Manipal Academy of Higher Education, Manipal, India.
3Department of Urology, Kasturba Medical College, Manipal Academy of Higher Education, Manipal, India.
4Department of Biochemistry, Kasturba Medical College, Mangalore, Manipal Academy of Higher Education, Manipal, India.
*Corresponding Author
Published In:
Volume - 16,
Issue - 5,
Year - 2023
ABSTRACT:
Introduction: Bisphenol A (BPA) is considered one of the most common industrial manufactured chemicals. Vitamin C is an important dietary antioxidant which significantly decreases the adverse effects of reactive oxygen species. Objective of the study: To evaluate the damaging effect of Bisphenol on testes and the role of vitamin C on Bisphenol induced testicular damage. Materials and Methods: Institutional ethics committee clearance was obtained before beginning the experiments. Animals were divided into the following groups with 6 rats in each group; Group 1: Control Group (Olive oil treated) Group 2: (Vitamin C treated), Group 3: (Bispenol treated), Group 4: (Vitamin C + Bispenol treated). Testicular tissue was processed to measure MDA and GSH level, sperm count and sperm morphology, histology. Serum level of testosterone was estimated. Results: Results of the present study showed a significant decrease in the level of GSH and sperm count and testosterone level and increase in MDA in Group 3 compared to GR.1and GR.2. Pre-treatment with Vitamin C showed an increase in the level of GSH and testosterone as well as sperm count compared to Bisphenol treated rats. Histological results were supported the biochemical results. Vitamin C also showed decrease in sperm shape abnormality which was induced by Bisphenol intoxication. Conclusion: Thus we have hypothesized that vitamin C could act as an antioxidant against BPA induced oxidative stress.
Cite this article:
Sumit Kumar, Rekha D Kini, Nayanatara Arun Kumar, Megha Gokul, Vivek Pai M, Shyamala Nayak. Potential role of Vitamin C as an Antioxidant on Bisphenol (BPA) Induced Oxidative Stress in Wistar rats. Research Journal of Pharmacy and Technology 2023; 16(5):2142-6. doi: 10.52711/0974-360X.2023.00352
Cite(Electronic):
Sumit Kumar, Rekha D Kini, Nayanatara Arun Kumar, Megha Gokul, Vivek Pai M, Shyamala Nayak. Potential role of Vitamin C as an Antioxidant on Bisphenol (BPA) Induced Oxidative Stress in Wistar rats. Research Journal of Pharmacy and Technology 2023; 16(5):2142-6. doi: 10.52711/0974-360X.2023.00352 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2023-16-5-11
REFERENCES:
1. Barnham K.J., Masters C.L.Bush A.I..Neurodegenerative diseases and oxidative stress. Nat. Rev. Drug Discov.2004; 3(3):205–214. doi: 10.1038/nrd1330
2. Khan, N.G., Correia, J., Adiga, D. et al. A comprehensive review on the carcinogenic potential of bisphenol A: clues and evidence. Environ Sci Pollut Res .2021 April;28: 19643–19663.doi:10.1007/s11356-021-13071-w. Epub 2021 Mar 5.
3. .Amardip M. Patil.(2018).Synthesis, Characterizations and Application of Bisphenol-A Based Highly Branched Polyol in Polyurethanes Coatings. Asian J. Research Chem. 2018; 11(3):593-598.doi: 10.5958/0974-4150.2018.00106.2
4. Morgan A.M., El-Ballal S.S., El-Bialy B.E., El-Borai N.B. Studies on the potential protective effect of cinnamon against bisphenol A- and octylphenol-induced oxidative stress in male albino rats. Toxicol. Rep. 2014 May;1: 92–101. doi: 10.1016/j.toxrep.2014.04.003. eCollection 2014
5. Federica Cariati, Nadja D’Uonno, Francesca Borrillo, Stefania Iervolino, Giacomo Galdiero, Rossella Tomaiuolo. Bisphenol a: an emerging threat to male fertility. Reprod Biol Endocrinol. 2019 January(20); 17: 6. doi: 10.1186/s12958-018-0447-6.
6. Helal E, Badawi M, Soliman M et al. Physiological and Histopathological studies on Bisphenol-A compound as xenoestrogen in male albino rats. The Egyptian Journal of Hospital Medicine. 2013 Jan;. 50: 127 – 136.DOI: 10.21608/EJHM.2018.16081
7. Ellman GL. Tissue sulfhydryl groups. Arch Biochem Biophys. 1959;82(1):70-77.doi.org/10.1016/0003-9861(59)90090-6
8. Rao GM, Rao AV, Raja A, Rao S, Rao A. Lipid peroxidation in brain tumours. Clin Chim Acta. 2000 Dec;302(1-2):205-211.doi: 10.1016/s0009-8981(00)00330-2
9. K. Narayana, U.J.A. D’Souza, K.P.S. Rao. Effect of ribavirin on epididymal sperm count in rat, Indian J. Physiol. Pharmacol .2002 Jan; 46(1) :97–101. doi: 10.22074/cellj.2021.7982.
10. K. Narayana, U.J.A. D’Souza, K.P.S. Rao. Ribavirin induced sperm shape abnormalities in Wistar rat, Mutat. Res. 2002 Jan 15; 513 (1–2): 193–196. doi: 10.1016/s1383-5718(01)00308-4.
11. Culling C F A,Allison RT, Batt WT. Cellular pathology techniques,14th edition, London: Butter worths,1985.
12. Induction of oxidative stress by bisphenol A in the epididymal sperm of rats. KC Chitra,C. Latchoumycandane ,PP Mathur. Toxicology.2003 Mar 14;185(1-2):119-27. doi: 10.1016/s0300-483x(02)00597-8.
13. Karafakioglu, Y.S., Tic, Aslan, R. Taurine prevents nonylphenol- induced oxidative Stress in rats. J. Anim. Vet. Adv.2010; 9 (1): 37–43.doi: 10.3923/javaa.2010.37.43
14. Vandana Gautam, Dhriti Kapoor, Saroj Arora, Renu Bhardwaj. Characterization and Antioxidant Activity of oil Extract of a Gymnosperm - Araucaria cunninghamii Aiton ex D. Don Research J. Pharm. and Tech. 2016; 9(7): 875-879. 21. doi: 10.5958/0974-360X.2016.00166.9
15. Pavithra. S, N. Banu. Free Radical Scavenging Activity and Total Antioxidant Capacity of Tin Chlorophyllin from Morinda citrifolia L. Research J. Pharm. and Tech. 2017; 10(2): 453-455.doi; 10.5958/0974-360X.2017.00091.9
16. Smitha Sammith Shetty, Darrien Ray Quays, Justina John, Ng See Yee, Daneshwary a/p Appu, Lim Meng Tze, Tee Shin Chuen, Ahaliaa a/p Sachchithanantham, Sacha Miriam Augustus. Effect of Oil pulling on Oral Health- A Microbiological Study. Research J. Pharm. and Tech 2019; 12(1): 01-04.doi:10.5958/0974-360X.2019.00001.5
17. A.V. Jaydeokar, D.D. Bandawane, S.S. Nipate and P.D. Chaudhari..Natural Antioxidants: A Review on Therapeutic Applications. Research J. Pharmacology and Pharmacodynamics. 2012 Ian; 4(1): 55-61.(doi-not available)
18. Moon, M.K., Kim, M.J., Jung, I.K., Koo, Y.D., Ann, H.Y., Lee, K.J.,Kim, S.H., Yoon, Y.C., Cho, B.J., Park, K.S., Jang, H.C., Park, Y.J., . Bisphenol A impairs mitochondrial function in the liver at doses below the no observed adverse effect level. J. Korean Med. Sci. 2012;27: 644–652.doi: 10.3346/jkms.2012.27.6.644
19. Kabuto, H., Hasuike, S., Minagawa, N., Shishibori, T. Effects of bisphenol A on the metabolisms of active oxygen species in mouse tissues. Environ. Res. 2003 Sep;93: 31–35.doi: 10.1016/s0013-9351(03)00062-8.
20. Rekha D. Kini, Tripathi Y., C.V. Raghuveer, Sheila R. Pai. Effect of Vitamin E Pre-Treatment on Histopathological Changes on Rat Testis Following Cadmium Chloride Administration. Research J. Pharm. and Tech.2013 August; 6(8): Page 874-877.(doi-not available)
21. Leem YH, Oh S, Kang HJ, Kim JH, Yoon J, Chang JS. . (2016 )BPA-toxicity via superoxide anion overload and a deficit in beta-catenin signaling in human bone mesenchymal stem cells.2017 Jan; 32(1): 344–352.doi: 10.1002/tox.22239
22. Natalie R. Gassman.Induction of oxidative stress by bisphenol A and its pleiotropic effects. Environ Mol Mutagen.2017 Mar;58(2):60-71.doi:10.1002/em.22072. Epub 2017 Feb 9.
23. Ooe H, Taira T, Iguchi-Ariga SM, Ariga H. Induction of reactive oxygen species by bisphenol A and abrogation of bisphenol A-induced cell injury by DJ-1. Toxicol Sci.2005 Nov;88(1):114-26.doi: 10.1093/toxsci/kfi278. Epub 2005 Aug 10.
24. Karageorgos, N., Patsoukis, NcChroni, E., Konstantinou, D., Assimakopoulos,S.F., Georgioui, C. Effect of N acetylcysteine, allopurinol and vitamin E on jaundice-induced brain oxidative stress in rats. Brain Res.2006 Sep 21;1111(1):203-12.doi: 10.1016/j.brainres.2006.06.088. Epub 2006 Aug 1
25. Rupali Kirtawade, Pallavi Salve, Anita Kulkarni and Pandurang Dhabale.Herbal antioxidant: Vitamin C. Research J. Pharm. and Tech. 2009;3(1): 58-61.doi:not available
26. Pooja V. Jagasia, Divyadarshani B.Spectrophotometric Determination of Vitamin C in Pharmaceutical Preparations using Ammonium Metavanadate. Asian J. Research Chem. 2017; 10(3):341-344.doi:10.5958/0974-4150.2017.00057.8
27. S. T. Dengle1, S. T. Gaikwad, A. S. Rajbhoj. Potentiometric study of vitamin C (Ascorbic acid) with transition metals in MeOH-water and EtOH-water medium. Asian Journal of Research in Chemistry. 2012;.5,;963-965.doi: not available
28. Dogan, A., Kolankaya, D. Protective effect of Anzer honey against ethanolinduced increased vascular permeability in the rat stomach. Exp. Toxicol. Pathol. 2005 Nov;57(2):173-8.doi: 10.1016/j.etp.2005.04.004. Epub 2005 Jun 17