ABSTRACT:
The current study is designed to investigate the immunoprotective efficacy of ashwagandha against cypermethrin-induced spleen toxicity in mice. Animals were divided randomly into four groups with six mice per group. Group, I served as control, group II cypermethrin treated, group III cypermethrin and ashwagandha co-treated, and group IV ashwagandha treated, all doses are given orally for 28 days. Samples were collected at the end of the experiment and later biochemical, histological, and hematological parameters were analyzed. A significant decrease was seen in the hematological parameters like hemoglobin percentage (Hb%), RBC, WBC, and MCH. The biochemical parameters like lipid peroxidation (*LPO) (p<0.05), H2O2 (**p<0.01), Nitric oxide (NO), and Catalase (**p<0.01), increased significantly and the Glutathione-S-transferase (GST) (**p<0.01), Glutathione reductase and Superoxide dismutase (**p<0.01) decreased significantly in cypermethrin exposed group as compared to control. The co-treatment with ashwagandha showed significant prevention in hematological and biochemical parameters. The histopathological investigation shows, the increased toxicity in the spleen of the cypermethrin exposed group as compared to the control and ashwagandha played a preventive role in the case of cypermethrin toxicity.