Author(s): N.N. Gavrilova, I.A. Ratnikova, A.K. Sadanov, S.E. Orazymbet, E.Zh. Shorabaev, B.B. Baimakhanova, L.E. Protasiuk

Email(s): iratnikova@list.ru

DOI: 10.52711/0974-360X.2023.00682   

Address: N.N. Gavrilova1, I.A. Ratnikova1*, A.K. Sadanov1, S.E. Orazymbet1, E.Zh. Shorabaev2, B.B. Baimakhanova1, L.E. Protasiuk1
1Research and Production Center for Microbiology and Virology, 105 Bogenbai Batyr str., Almaty, 050010, Kazakhstan.
2Industrial Microbiology LLP, 89/44 Al-Farabi ave., Almaty, 050060, Kazakhstan.
*Corresponding Author

Published In:   Volume - 16,      Issue - 9,     Year - 2023


ABSTRACT:
Studies were conducted on the toxicity and possible sensitizing effect of the lyophilizate of a therapeutic probiotic agent from the most active strains of lactic acid and propionic acid bacteria isolated from healthy people: L. plantarum 2v/A-6+L. brevis B-3/A-26+L. acidophilus 27w/60+P. shermanii 8. Based on the studies conducted to evaluate acute toxicity with an oral single administration of probiotic agent solutions (lyophilizate of lactic acid and propionic acid bacteria) at doses of 300, 2,000, 4,000, and 5,001mg/kg of weight, the toxicity class of the preparation under study has been defined as a non-toxic substance. In the study on laboratory mice of subchronic (subacute) toxicity of probiotic agent (lyophilizate of lactic acid and propionic acid bacteria)) in doses of 5.0×106, 10.0×106, and 15.0×106 CFU, it has been found that the preparation does not have a toxic effect on the body in case of 28-day use. Studies of the chronic toxicity of the probiotic agent (lyophilizate of lactic acid and propionic acid bacteria) in doses of 5.0×106, 10.0×106, and 15.0×106 CFU, in laboratory mice have shown that this preparation does not have a toxic effect on the body in case of prolonged 60-day use and does not cause structural changes in internal organs. It has been established that the therapeutic probiotic agent (lyophilizate of lactic acid and propionic acid bacteria) does not have a potential sensitizing (allergenic) effect on rat peritoneal mast cells.


Cite this article:
N.N. Gavrilova, I.A. Ratnikova, A.K. Sadanov, S.E. Orazymbet, E.Zh. Shorabaev, B.B. Baimakhanova, L.E. Protasiuk. Determination of the Toxicity and Sensitizing effect of a Therapeutic Probiotic agent against Intestinal and Concomitant human infections. Research Journal of Pharmacy and Technology 2023; 16(9):4161-1. doi: 10.52711/0974-360X.2023.00682

Cite(Electronic):
N.N. Gavrilova, I.A. Ratnikova, A.K. Sadanov, S.E. Orazymbet, E.Zh. Shorabaev, B.B. Baimakhanova, L.E. Protasiuk. Determination of the Toxicity and Sensitizing effect of a Therapeutic Probiotic agent against Intestinal and Concomitant human infections. Research Journal of Pharmacy and Technology 2023; 16(9):4161-1. doi: 10.52711/0974-360X.2023.00682   Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2023-16-9-26


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