Author(s):
Mayada Alwattar, Naeem Shalan, Mohd Alaraj
Email(s):
m.araj@jpu.edu.jo
DOI:
10.52711/0974-360X.2023.00684 
Address:
Mayada Alwattar1, Naeem Shalan1, Mohd Alaraj2,3*
1Pharmacological and Diagnostic Research Centre (PDRC), Faculty of Pharmacy, Al-Ahliyya Amman 19328, Jordan.
2Faculty of Pharmacy, University of Jerash.
3Faculty of Pharmacy, Middle East University, Jordan.
*Corresponding Author
Published In:
Volume - 16,
Issue - 9,
Year - 2023
ABSTRACT:
Gemcitabine (GEM) is one of the most widely used chemotherapeutic medications for treating various solid tumuors. Niosomes are a novel drug delivery system, self-assembled vesicular nanocarriers and composed of several surfactants and various lipids. Date seed Oil (DSO) contain a high percentage of phenolic and flavonoid compounds that making them important for food and pharmaceutical formulations. In this study, we prepared GEM loaded into anionic niosomes (GEM-niosomes) to target breast tumor cells. The niosomes were prepared with cholesterol (CHOL), span 60, date seed oil and chloroform using the thin film hydration method. GEM-niosomes were fully characterized for their physiochemical properties and evaluated for their cytotoxicity. The targeted niosomes were 100±10nm, the loaded niosomes were in the range of 125±15. GEM-fully loaded DSO-niosomes with drug entrapment percentage (EE%) 82% using high- performance liquid chromatography (HPLC) were developed. The prepared targeted GEM-niosomes showed good stability over 4 weeks at -7ºC and better drug loading. Our targeted GEM-niosomes showed stronger activity against MCF-7 cell by approximately 10 folds compared with the free drug. Taken together, the combination of GEM and “DSO- loaded niosomes” may be of great importance for developing new treatments following in vivo investigations with breast cancer animal models. Future research should explore the in vitro and the in vivo cytotoxicity of this combination on more aggressive breast cancer ( BC).
Cite this article:
Mayada Alwattar, Naeem Shalan, Mohd Alaraj. Anticancer Effects of Date Seed Oil- Loaded Niosomes and Gemcitabine Combination in MCF-7 Human Breast Cancer Cells. Research Journal of Pharmacy and Technology 2023; 16(9):4179-7. doi: 10.52711/0974-360X.2023.00684
Cite(Electronic):
Mayada Alwattar, Naeem Shalan, Mohd Alaraj. Anticancer Effects of Date Seed Oil- Loaded Niosomes and Gemcitabine Combination in MCF-7 Human Breast Cancer Cells. Research Journal of Pharmacy and Technology 2023; 16(9):4179-7. doi: 10.52711/0974-360X.2023.00684 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2023-16-9-28
REFERENCES:
1. V. N. Dange, S. J. Shid, C.S. Magdum, S.K. Mohite. A Review on Breast cancer: An Overview. Asian J. Pharm. Res. 2017; 7(1): 49-51.
2. Scholl AR, Flanagan MB. Educational Case: Invasive Ductal Carcinoma of the Breast. Academic Pathology 2020; 7: 2374289519897390. https://doi.org/10.1177/2374289519897390
3. Silvestris N, Cinieri S, La Torre I, Pezzella G, Numico G, Orlando L, Lorusso V. Role of gemcitabine in metastatic breast cancer patients: A short review. Breast 2008; 17(3): 220–226. https://doi.org/10.1016/j.breast.2007.10.009.
4. Majed Jary Mohammed, Zeyad Kadhim Oleiwi, Muntadher Abdulabbas Hasan Al-Hilo, Ahmed Kareem Hussein Mubarak, Ehab Kareem Obaid, Ali Jabbar Radhi. Synthesis and Study Biological Activity of Gemcitabine Linked Heterocyclic Hybrids. Research J. Pharm. and Tech. 2020; 13(7): 3257-3261.
5. Markman JL, Rekechenetskiy A, Holler E, Ljubimova JY. Nanomedicine therapeutic approaches to overcome cancer drug resistance. Advanced Drug Delivery Reviews 2013; 65(13–14): 1866–1879. https://doi.org/10.1016/j.addr.2013.09.019
6. Kshitij B. Makeshwar, Suraj R. Wasankar. Niosome: a Novel Drug Delivery System. Asian J. Pharm. Res. 3(1): Jan.-Mar. 2013; Page 15-19..
7. Yap KM, Sekar M, Fuloria S, Wu YS, Gan SH, Mat Rani NNI, Subramaniyan V, Kokare C, Teng Lum P, Begum MY, Mani S, Meenakshi DU, Sathasivam KV, Fuloria NK. Drug Delivery of Natural Products Through Nanocarriers for Effective Breast Cancer Therapy: A Comprehensive Review of Literature. International Journal of Nanomedicine 2021; 16(November): 7891–7941. https://doi.org/10.2147/ijn.s328135
8. Taranjit Kaur, Charanjit Kaur, Iqbaljit Kaur, Parminderjit Kaur. Preparation and characterization of In Situ Gel of Gemcitabine Hydrochloride loaded Nanoparticles used for the treatment of Pancreatic Cancer. Research Journal of Pharmacy and Technology. 2021; 14(12):6609-6.
9. M Lavanya, Asish Bhaumik, A Gopi Reddy, Ch Manasa, B Kalyani, S Sushmitha. Evaluation of Anticancer Activity of Ethanolic and Ethylacetoacetate Extracts of Sweet Cherry Against Human Breast Cancer Cell Line MCF-7. Research J. Pharmacology and Pharmacodynamics.2016; 8(2): 65-70
10. Disher, I., Ali, M., & Alhattab, T. Extraction of Date Palm Seed Oil (Phoenix Dactylifera) by Soxhlet Apparatus. International Journal of Advances in Engineering & Technology (IJAET) 2015; 8, 261–271.
11. Ahsan H, Ahad A, Iqbal J, Siddiqui WA. Pharmacological potential of tocotrienols: a review. Nutrition & Metabolism 2014; 11(1): 1-22. https://doi.org/10.1186/1743-7075-11-52.
12. Abdeldjabbar Messaoudi, Messaouda Dekmouche, Zehour Rahmani, Cheyma Bensaci. Phenolic profile, Antioxidant potential of date (Phoenix dactylifera Var. Degla Baidha and Deglet-Nour) seeds from Debila region (Oued Souf, Algeria). Asian J. Research Chem. 2021; 14(1):37-41.
13. Kim DO, Jeong SW, Lee CY (2003) Antioxidant capacity of phenolic phytochemicals from various cultivars of plums. Food Chemistry 2003; 81(3): 321–326. https://doi.org/https://doi.org/10.1016/S0308-8146(02)00423-5
14. Saafi, E, Amira EA, Issaoui M, Hammami M, Achour L (2009) Phenolic content and antioxidant activity of four date palm (Phoenix dactylifera L.) fruit varieties grown in Tunisia. International Journal of Food Science & Technology 2009; 44(11): 2314–2319. https://doi.org/10.1111/j.1365-2621.2009.02075.x
15. Kushnazarova RA, Mirgorodskaya AB, Zakharova LY. Niosomes modified with cationic surfactants to increase the bioavailability and stability of indomethacin. Russian Chemical Bulletin 2021; 70(3): 585-591. https://doi.org/10.1007/s11172-021-3129-z
16. De Silva L, Fu JY, Htar TT, Muniyandy S, Kasbollah A, Wan Kamal WHB, Chuah LH. Characterization, optimization, and in vitro evaluation of Technetium-99m-labeled niosomes. International Journal of Nanomedicine 2019; 14: 1101–1117. https://doi.org/10.2147/IJN.S184912.
17. Charde M, Shinde M, Welankiwar A, Jitendra K. Development of analytical and stability testing method for vitamin A palmitate formulation. International Journal of Pharmaceutical Chemistry 2015; 5(4): 104–114. https://doi.org/10.7439/ijpc
18. Stepanenko AA, Dmitrenko VV. HEK293 in cell biology and cancer research: phenotype, karyotype, tumorigenicity, and stress-induced genome-phenotype evolution. Gene 2015; 569(2): 182-190. https://doi.org/10.1016/j.gene.2015.05.065
19. Mosmann T. Rapid Colorimetric Assay for Cellular Growth and Survival: Application to Proliferation and Cytotoxicity Assays. Journal Of lmmunological Methods 1983; 65(1-2): 55–63. https://doi.org/10.1039/c6ra17788c
20. Grimmig R, Babczyk P, Gillemot P, Schmitz KP, Schulze M, Tobiasch E. Development and evaluation of a prototype scratch apparatus for wound assays adjustable to different forces and substrates. Applied Sciences 2019; 9(20): 4414. https://doi.org/10.3390/app9204414
21. Roma Mathew, Joyamma Varkey. Development of Solid Self Nanoemulsifying Drug Delivery System of Quercetin. Asian Journal of Research in Pharmaceutical Sciences. 2022; 12(3):183-8.
22. Gharbavi M, Amani J, Kheiri-Manjili H, Danafar H, Sharafi A. Niosome: A Promising Nanocarrier for Natural Drug Delivery through Blood Brain Barrier. Advances in Pharmacological Sciences 2018; 2018. https://doi.org/10.1155/2018/6847971
23. Alireza Doroudi, Ehsan Rezaee, Seyyed Mostafa Saadati, Ali Kiasat, Faramarz Ahmadi, Behrooz Etesami, Mostafa Erfani. The Role of Sonication for the Preparation of Infection-Seeking Radiotracer Sample Versus the Standard Method. Research J. Pharm. and Tech. 2018; 11(3): 987-995.
24. Clogston JD, Patri AK. Zeta potential measurement. Characterization of nanoparticles intended for drug delivery. Humana Press 2011; 697: 63–70. https://doi.org/10.1007/978-1-60327-198-1_6
25. Sezgin-Bayindir Z, Yuksel N. Investigation of Formulation Variables and Excipient Interaction on the Production of Niosomes. AAPS PharmSciTech 2012; 13(3): 826–835. https://doi.org/10.1208/s12249-012-9805-4
26. Nowroozi F, Almasi A, Javidi J, Haeri A, Dadashzadeh S. Effect of Surfactant Type, Cholesterol Content and Various Downsizing Methods on the Particle Size of Niosomes. Iranian Journal of Pharmaceutical Research 2018; 17(Suppl2): 1–11. PMID: 31011337; PMCID: PMC6447874.
27. Yeo LK, Chaw CS, Elkordy AA. The Effects of Hydration Parameters and Co-Surfactants on Methylene Blue-Loaded Niosomes Prepared by the Thin Film Hydration Method. Pharmaceuticals 2019; 12(2): 46. https://doi.org/10.3390/ph12020046
28. Khan DH, Bashir S, Figueiredo P, Santos HA, Khan MI, Peltonen L Process optimization of ecological probe sonication technique for production of rifampicin loaded niosomes. Journal of Drug Delivery Science and Technology 2019; 50: 27–33. https://doi.org/https://doi.org/10.1016/j.jddst.2019.01.012
29. Hnin HM, Stefánsson E, Loftsson T, Asasutjarit R, Charnvanich D, Jansook P. Physicochemical and Stability Evaluation of Topical Niosomal Encapsulating Fosinopril/γ-Cyclodextrin Complex for Ocular Delivery. Pharmaceutics 2022; 14(6) :1147. https://doi.org/10.3390/pharmaceutics14061147
30. Ahad A, Raish M, Al-Jenoobi FI, Al-Mohizea AM. Sorbitane Monostearate and Cholesterol based Niosomes for Oral Delivery of Telmisartan. Current Drug Delivery 2018; 15(2): 260–266. https://doi.org/10.2174/1567201814666170518131934
31. Rasul A, Imran Khan M, Ur Rehman M, Abbas G, Aslam N, Ahmad S, Abbas K, Akhtar Shah P, Iqbal M, Al Subari AMA, Shaheer T, Shah S. In vitro Characterization and Release Studies of Combined Nonionic Surfactant-Based Vesicles for the Prolonged Delivery of an Immunosuppressant Model Drug. International Journal of Nanomedicine 2020; 15: 7937–7949. https://doi.org/10.2147/IJN.S268846
32. Mahawar Sheetal. Development and Characterization of Docetaxel Encapsulated pH-Sensitive Liposomes for Cancer Therapy. Research J. Pharma. Dosage Forms and Tech. 2013; 5(3): 151-160 .
33. Dabbagh Moghaddam F, Akbarzadeh I, Marzbankia E, Farid M, khaledi L, Reihani AH, Javidfar M, Mortazavi P. Delivery of melittin-loaded niosomes for breast cancer treatment: an in vitro and in vivo evaluation of anti-cancer effect. Cancer Nanotechnology 2021; 12(1): 1-35. https://doi.org/10.1186/s12645-021-00085-9
34. Zarrabi Ahrabi N, Tabaie SM, Jahanshiri M. Study of Cytotoxic Effects of Caffeine- Loaded Niosomes on Human Breast Cancer Cells MCF 7. Journal of Sabzevar University of Medical Sciences 2021; 28(5): 663–674. http://jsums.sinaweb.net/article_1447.html
35. Al-Sheddi, Ebtesam S. Anticancer potential of seed extract and pure compound from Phoenix dactylifera on human cancer cell lines. Pharmacognosy Magazine 2019; 15(63): 494-9. http://www.phcog.com/text.asp?2019/15/63/494/258401
36. Rezaei M, Khodaei F, Hooshmand N. Date Seed Extract Diminished Apoptosis Event in Human Colorectal Carcinoma Cell Line. MOJ Toxicol 2015; 1(4): 00017. DOI: 10.15406/mojt.2015.01.00017
37. L. Krishnasamy, M. Masilamani Selvam, Bharathi Ravikrishnan. Anticancer property of Colchicine isolated from Indigofera aspalathoids. Research J. Pharm. and Tech. 2016; 9(4): 386-390.
38. Kim O, Hwangbo C, Kim J, Li DH, Min BS, Lee JH (2015) Chelidonine suppresses migration and invasion of MDA-MB-231 cells by inhibiting formation of the integrin-linked kinase/PINCH/α-parvin complex. Molecular Medicine Reports 12(2): 2161–2168. https://doi.org/10.3892/mmr.2015.3621
39. Lü S, Wang J. Homoharringtonine and omacetaxine for myeloid hematological malignancies. Journal of Hematology & Oncology 2014; 7(1): https://doi.org/10.1186/1756-8722-7-2