Author(s): Mayada Alwattar, Naeem Shalan, Mohd Alaraj


DOI: 10.52711/0974-360X.2023.00684   

Address: Mayada Alwattar1, Naeem Shalan1, Mohd Alaraj2,3*
1Pharmacological and Diagnostic Research Centre (PDRC), Faculty of Pharmacy, Al-Ahliyya Amman 19328, Jordan.
2Faculty of Pharmacy, University of Jerash.
3Faculty of Pharmacy, Middle East University, Jordan.
*Corresponding Author

Published In:   Volume - 16,      Issue - 9,     Year - 2023

Gemcitabine (GEM) is one of the most widely used chemotherapeutic medications for treating various solid tumuors. Niosomes are a novel drug delivery system, self-assembled vesicular nanocarriers and composed of several surfactants and various lipids. Date seed Oil (DSO) contain a high percentage of phenolic and flavonoid compounds that making them important for food and pharmaceutical formulations. In this study, we prepared GEM loaded into anionic niosomes (GEM-niosomes) to target breast tumor cells. The niosomes were prepared with cholesterol (CHOL), span 60, date seed oil and chloroform using the thin film hydration method. GEM-niosomes were fully characterized for their physiochemical properties and evaluated for their cytotoxicity. The targeted niosomes were 100±10nm, the loaded niosomes were in the range of 125±15. GEM-fully loaded DSO-niosomes with drug entrapment percentage (EE%) 82% using high- performance liquid chromatography (HPLC) were developed. The prepared targeted GEM-niosomes showed good stability over 4 weeks at -7ºC and better drug loading. Our targeted GEM-niosomes showed stronger activity against MCF-7 cell by approximately 10 folds compared with the free drug. Taken together, the combination of GEM and “DSO- loaded niosomes” may be of great importance for developing new treatments following in vivo investigations with breast cancer animal models. Future research should explore the in vitro and the in vivo cytotoxicity of this combination on more aggressive breast cancer ( BC).

Cite this article:
Mayada Alwattar, Naeem Shalan, Mohd Alaraj. Anticancer Effects of Date Seed Oil- Loaded Niosomes and Gemcitabine Combination in MCF-7 Human Breast Cancer Cells. Research Journal of Pharmacy and Technology 2023; 16(9):4179-7. doi: 10.52711/0974-360X.2023.00684

Mayada Alwattar, Naeem Shalan, Mohd Alaraj. Anticancer Effects of Date Seed Oil- Loaded Niosomes and Gemcitabine Combination in MCF-7 Human Breast Cancer Cells. Research Journal of Pharmacy and Technology 2023; 16(9):4179-7. doi: 10.52711/0974-360X.2023.00684   Available on:

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