Author(s): Kancharla Bhanukiran, Tarkeshwar Dubey, Siva Hemalatha

Email(s): shemalatha.phe@itbhu.ac.in

DOI: 10.52711/0974-360X.2023.00694   

Address: Kancharla Bhanukiran, Tarkeshwar Dubey, Siva Hemalatha*
Department of Pharmaceutical Engineering and Technology, Indian Institute of Technology, Banaras Hindu University, Varanasi, India.
*Corresponding Author

Published In:   Volume - 16,      Issue - 9,     Year - 2023


ABSTRACT:
Marsilea quadrifolia is traditionally used for treatment of diabetes by the natives of Jharkhand in India. The current study is focused on optimization of extraction process and validation of traditional claim of the plant Marsilea quadrifolia Lin streptozotocin-nicotinamide induced type2 diabetic rats.Box Behnken Design (BBD) software was used for optimization of extraction process and total phenolic content of the plant. Antidiabetic potential of hydroalcoholic extract (150mg/kg, 300mg/kg, 450mg/kg) was evaluated through in-vivo rat model, and diabetes was induced by an intraperitoneal injection of nicotinamide (110mg/kg, i.p.) followed by streptozotocin injection (65mg/kg, i.p.). Glibenclamide (10mg/kg, per oral) was used as standard drug during treatment. Extractive yield and total phenolic content were found to be 14.1% and 119.45mg/g gallic acid of dried extract, respectively. The hydroalcoholic extract of M. quadrifolia exhibited significant reduction in blood glucose level at a dose of 150mg/kg, 300mg/kg, and 450mg/kg in a dose-dependent manner when compared to standard Glibenclamide (10mg/kg, per oral). Moreover, extract showed improvement in biochemical parameters, such as lipid profile, body weight, liver glycogen, and in-vivo antioxidant potency. Furthermore, histopathological examinations were performed on rat brains. Our work potentially validates the traditional claim of plant being used as anti-diabetic, using STZ induced rat model. The total phenolic content of hydroalcoholic extract of M. quadrifolia L. may be responsible for its anti-diabetic potential.


Cite this article:
Kancharla Bhanukiran, Tarkeshwar Dubey, Siva Hemalatha. Optimization of Extraction Process and Preclinical Evaluation of Marsilea quadrifolia L. in Streptozotocin-Induced Diabetic Rats. Research Journal of Pharmacy and Technology 2023; 16(9):4239-6. doi: 10.52711/0974-360X.2023.00694

Cite(Electronic):
Kancharla Bhanukiran, Tarkeshwar Dubey, Siva Hemalatha. Optimization of Extraction Process and Preclinical Evaluation of Marsilea quadrifolia L. in Streptozotocin-Induced Diabetic Rats. Research Journal of Pharmacy and Technology 2023; 16(9):4239-6. doi: 10.52711/0974-360X.2023.00694   Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2023-16-9-38


REFERENCES:
1.    Devi M, Ramesh B. Hypoglycemic activity of Leaves of Bougainvillea spectabilis extract in Streptozotocin-Induced Diabetic Rats. Asian Journal of Pharmaceutical Research. 2018;8(2):99-103.https://doi.org/10.5958/2231-5691.2018.00017.5
2.    Sisode PS, Jain VC, Prajapati N. Pharmacology of combined treatment of saxagliptin hydrochloride and glibenclamide therapy to treat type-2 diabetes. Asian Journal of Research in Pharmaceutical Science. 2016;6(1):59-61.https://doi.org/10.5958/2231-5659.2016.00009.6
3.    Sahua S, Duttaa G, Mandala N, Goswamia AR. Ghosha. T.: Anticonvulsant effect of Marsilea quadrifolia Linn. on pentylenetetrazole induced seizure: A behavioral and EEG study in rats. J Ethnopharmacol. 2012;141:537-51. https://doi.org/10.1016/j.jep.2012.02.039
4.    Snehunsu A, Ghosal C, Kandwal M, Yadav PK, Nayak BS, Rao KR, et al. 1-Triacontanol cerotate; isolated from Marsilea quadrifolia Linn. ameliorates reactive oxidative damage in the frontal cortex and hippocampus of chronic epileptic rats. J Ethnopharmacol. 2015;172:80-4. https://doi.org/10.1016/j.jep.2015.06.020
5.    Uma R, Pravin B. In vitro cytotoxic activity of Marsilea quadrifolia Linn. of MCF-7 cells of human breast cancer. Int Res J Medical Sci I. 2013;1(1):10-3.
6.    Gini T, Jothi GJ. In vitro screening of antibacterial and antifungal activity of Marsilea quadrifolia (Marsileaceae) Linn. extract. Am J Phytomed Clin Therap. 2015;3:313-29.
7.    Rajendran K, Vijayabharathi R. Anti-hyperglycemic activity of ethanol leaf extract of Marsilea quadrifolia Linn. Hamdard Medicus (Pakistan). 2005.
8.    Zahan R, Ripa FA, Alam MB, Haque MA, Mosaddik M, Nahar L. Hypoglycemic and in vitro antioxidant effects of methanolic extract of Marsilea quadrifolia plant. Pharmacognosy Journal. 2011;3(26):86-92. https://doi.org/10.5530/pj.2011.26.14
9.    Dongare SS, Maske A, Patil S, Umbare R, Mate G. Antidiabetic Activity of Marsilea quadrifolia linn in alloxan-diabetic rats. Research Journal of Pharmacognosy and Phytochemistry. 2010;2(6):15-7.  https://doi.org/10.5958/0975-4385
10.    Chakraborty NR, Duary B. Utilization of some weeds as medicine by the local people in Birbhum District of West Bengal, India. International Journal of Bio-resource and Stress Management. 2014;5(1):148-52. https://doi.org/10.5958/j.0976-4038.5.1.029
11.    Saraf A, Dubey N, Dubey N, Sharma M. Box Behnken design based development of curcumin loaded Eudragit S100 nanoparticles for site-spcific delivery in colon cancer. Research Journal of Pharmacy and Technology. 2019;12(8):3672-8. https://doi.org/10.5958/0974-360X.2019.00627.9
12.    Guideline O. 425, Acute Oral Toxicity up and-Down Procedure in OECD Guidelines for the Testing of Chemicals, Organization for Economic Cooperation and Development. Paris, OECD; 2001. https://doi.org/10.1787/9789264071049-en.
13.    Shanmuga Sundaram C, Subramanian S. Biochemical evaluation of hypoglycemic activity of Musa paradisiaca (plantain) flowers in STZ-induced experimental diabetes in rats. Asian J Research Chem. 2011;4(5):827-33.
14.    Jain KA. A Mechanistic Approach to Determination of Anti-diabetic activity of Calystegia sepium R. Br. Flowering plants in normal and Streptozotocin induced rats. Asian Journal of Research in Pharmaceutical Science. 2014;4(2):55-70. https://doi.org/10.52711/2231-5659
15.    Nalla S, Goli V, Sabat M, Komati S, Begam N, Kokkirala VR. Salubrious Effect of Ethanolic Extract of Cassia auriculata Linn., in Streptazotocin-Nicotinamide induced Diabetes in Rat Model. Asian Journal of Pharmacy and Technology. 2012;2(3):104-6.
16.    Manivasagam TT, Dakshayani K, Subash SS, Sivaperumal RR. Protective influence of Hibiscus sabdariffa, an edible medicinal plant, on tissue lipid peroxidation and antioxidant status in hyperammonemic rats. African Journal of Traditional, Complementary and Alternative Medicines. 2006;3(3):10-21. https://doi.org/10.4314/ajtcam.v3i3.31163
17.    Manikandan P, Vidjaya Letchoumy P, Prathiba D, Nagini S. Combinatorial chemopreventive effect of Azadirachta indica and Ocimum sanctum on oxidant-antioxidant status, cell proliferation, apoptosis and angiogenesis in a rat forestomach carcinogenesis model. Singapore Med J. 2008;49(10):814.
18.    Pattabiraman K, Muthukumaran P. Antidiabetic and antioxidant activity of Morinda tinctoria roxb fruits extract in streptozotocin-induced diabetic rats. Asian J Pharm Tech. 2011;1(2):34-9. https://doi.org/10.5958/2231–5713
19.    Masiello P, Broca C, Gross R, Roye M, Manteghetti M, Hillaire-Buys D, et al. Experimental NIDDM: development of a new model in adult rats administered streptozotocin and nicotinamide. Diabetes. 1998;47(2):224-9. https://doi.org/10.2337/diab.47.2.224
20.    Hemalatha S, Wahi A, Singh P, Chansouria J. Hypoglycemic activity of Withania coagulans Dunal in streptozotocin induced diabetic rats. J Ethnopharmacol. 2004;93(2-3):261-4. https://doi.org/10.1016/j.jep.2004.03.043
21.    Vijayalakshmi M, Kiruthika R, Bharathi K, Ruckmani K. Phytochemical screening by LC-MS analysis and invitro anti-inflammatory activity of Marselia quadrifolia plant extract. International Journal of PharmTech Research. 2015;8(9):148-57.
22.    Pandeya SN, Kumar R, Kumar A, Pathak AK. Antidiabetics Review on Natural Products. Research Journal of Pharmacy and Technology. 2010;3(2):300-18. https://doi.org/10.5958/0974-360X
23.    Betteridge J. Lipid disorders in diabetes mellitus. Textbook of diabetes. 1997;31:1-55.
24.    Hassan NA, Karunakaran R, Uma SA, Aye KM. Effect of Zingiber officinale on Lipid profile of Sprague-Dawley rats induced with Streptozotocin. Research Journal of Pharmacy and Technology. 2020;13(8):3577-9. https://doi.org/10.5958/0974-360X.2020.00632.0
25.    Kumar R, Pate DK, Prasad SK, Sairam K, Hemalatha S. Antidiabetic activity of alcoholic leaves extract of Alangium lamarckii Thwaites on streptozotocin–nicotinamide induced type 2 diabetic rats. Asian Pac J Trop Med. 2011;4(11):904-9. https://doi.org/10.1016/S1995-7645(11)60216-2
26.    Jarald EE, Joshi S, Jain D. Antidiabetic activity of flower buds of Michelia champaca Linn. Indian J Pharmacol. 2008;40(6):256. https://www.ijp-online.com/text.asp?2008/40/6/256/45151
27.    Sophia D, Manoharan S. Hypolipidemic activities of Ficus racemosa Linn. bark in alloxan induced diabetic rats. African Journal of Traditional, Complementary and Alternative Medicines. 2007;4(3):279-88. https://doi.org/10.4314/ajtcam.v4i3.31220
28.    Virdi J, Sivakami S, Shahani S, Suthar A, Banavalikar M, Biyani M. Antihyperglycemic effects of three extracts from Momordica charantia. J Ethnopharmacol. 2003;88(1):107-11. https://doi.org/10.1016/s0378-8741(03)00184-3
29.    Mishra B, Kar DM, Maharana L, Dash S, Mishra GP. Hypoglycemic activity of methanol fraction of Tectona grandis (Linn) bark in experimental rat models. Research Journal of Pharmacy and Technology. 2021;14(8):4247-52. https://doi.org/10.52711/0974-360X.2021.00737
30.    Sharma N, Garg V, Paul A. Antihyperglycemic, antihyperlipidemic and antioxidative potential of Prosopis cineraria bark. Indian J Clin Biochem. 2010;25(2):193-200. https://doi.org/10.1007/s12291-010-0035-9
31.    Avijit M, Das S. Hepatoprotective nature of aerial parts of Caesalpinia pulcherrima in STZ Induced Diabetic Rat Model. Research Journal of Pharmacy and Technology. 2021;14(7):3716-20. https://doi.org/10.52711/0974-360X.2021.00643
32.    Balamurugan R, Ignacimuthu S. Antidiabetic and hypolipidemic effect of methanol extract of Lippia nodiflora L. in streptozotocin induced diabetic rats. Asian Pacific Journal of Tropical Biomedicine. 2011;1(1):S30-S6. https://doi.org/10.1016/S2221-1691(11)60117-2
33.    Murugan P, Pari L. Influence of tetrahydrocurcumin on erythrocyte membrane bound enzymes and antioxidant status in experimental type 2 diabetic rats. J Ethnopharmacol. 2007;113(3):479-86. https://doi.org/10.1016/j.jep.2007.07.004
34.    Patel D, Kumar R, Prasad S, Sairam K, Hemalatha S. Antidiabetic and in vitro antioxidant potential of Hybanthus enneaspermus (Linn) F. Muell in streptozotocin–induced diabetic rats. Asian Pacific journal of tropical biomedicine. 2011;1(4):316-22. https://doi.org/10.1016/S2221-1691(11)60051-8



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