Author(s):
Shiv Kumar Gupta, Neetu Sachan, Phool Chandra, Arun Kumar Sharma
Email(s):
shivki4u@gmail.com
DOI:
10.52711/0974-360X.2024.00728 
Address:
Shiv Kumar Gupta1*, Neetu Sachan2, Phool Chandra3, Arun Kumar Sharma4
1Saraswathi College of Pharmacy, NH-09/24, Anwarpur, Pilkhuwa, Hapur, Uttar Pradesh, India.
2Department of Pharmaceutical Sciences, Maharana Pratap Group of Institutions Mandhana Campus, 3 Kms from IIT Kanpur - 209217, Uttar Pradesh, India.
3College of Pharmacy Teerthankar University, N.H.-9, Delhi Road, Moradabad - 244001, Uttar Pradesh, India.
4Pharmacy Academy, IFTM University, NH-9, Lodhipur, Moradabad, 244001, Uttar Pradesh, India.
*Corresponding Author
Published In:
Volume - 17,
Issue - 10,
Year - 2024
ABSTRACT:
Irinotecan is an anti-cancer drug used for the treatment of ovarian cancer. It works by inhibiting DNA-topoisomerase, type I. A simple, accurate and precise assay and rapid stability indicating RP-HPLC method has been developed and subsequently validated for the quantitative estimation of Irinotecan in API and its parental preparation. Proposed method is based on identification of drug Irinotecan in reversed phase mode using water’s C18 column maintained at an ambient temperature. The optimum mobile phase consist of mixture of Acetonitrile: Mixed phosphate buffer (potassium dihydrogen phosphate (0.01mm) with 1.0ml triethylamine) (30:70v/v) and pH was adjusted to 2.5 with O-Phosphoric acid (1:100,v/v). The flow rate was maintained at 0.8 ml/min. The injection volume was 20ml and detected at the wavelength of 221nm. The method was validated according to ICH guideline. The method was found to be accurate, reproducible and linear. The drug was exposed to variable stressed conditions and was analyzed by proposed method. There were no interfering peaks from exicipients, impurities or degradative products due to variable stress conditions. Thus the proposed method is specific for the quantitative estimation of Irinotecan and can be successfully applied in the quality control and stability sample of API and its parental preparation.
Cite this article:
Shiv Kumar Gupta, Neetu Sachan, Phool Chandra, Arun Kumar Sharma. A Simple and Sophisticated RP-HPLC Approach for Quantifying of Irinotecan in API and in Pharmaceutical Dosage Form. Research Journal of Pharmacy and Technology. 2024; 17(10):4722-6. doi: 10.52711/0974-360X.2024.00728
Cite(Electronic):
Shiv Kumar Gupta, Neetu Sachan, Phool Chandra, Arun Kumar Sharma. A Simple and Sophisticated RP-HPLC Approach for Quantifying of Irinotecan in API and in Pharmaceutical Dosage Form. Research Journal of Pharmacy and Technology. 2024; 17(10):4722-6. doi: 10.52711/0974-360X.2024.00728 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2024-17-10-13
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