Author(s):
Dighe Pooja, Vikhe Sunayana, Jadhav Ravindra, Rahul L. Vikhe
Email(s):
sunainavikhe@gmail.com
DOI:
10.52711/0974-360X.2024.00730 
Address:
Dighe Pooja1, Vikhe Sunayana1*, Jadhav Ravindra1, Rahul L. Vikhe2
1Department of Pharmacognosy, Pravara Rural College of Pharmacy, Loni, (413736), Maharashtra, India.
2Department of Financial Management, Sir Vishveshwaraiya Institute of Technology, Sinnar, Maharashtra India.
*Corresponding Author
Published In:
Volume - 17,
Issue - 10,
Year - 2024
ABSTRACT:
Aim: Pharmacological Evaluation on effect of sour orange and Corn silk in combination on urolithiasis Ethylene Glycol induced rats. Objective: The present study was carried out to investigate the traditional use of Corn silk and Sour Orange easy removal of kidney stones due to its diuretic activity and action in rats Methods: The Reflux extraction method was used to obtain the aqueous extract, then column chromatography and TLC were used for fractionation. The fraction isolated are 2-Propanone, 1-hydroxy-,2-Furanmethanol, 1,3-Butadiene-1-carboxylic acid, has been confirmed by GC-HRMS results. Study design: The complex process of kidney stone formation, also known as urolithiasis, is brought on by a series of physicochemical processes that take place inside the kidneys, such as supersaturation, nucleation, growth, aggregation, and retention. In this present study antiurolithic activity of corn silk, sour orange, combined dose sour orange and corn silk, fraction extract investigated in animal models of urolithiasis. It is induced in rats by administering 0.75 % ethylene glycol in drinking water for 28 days and calcium oxalate crystals were confirmed by urine analysis and X rays. Then, urine analysis and X rays were done for crystals observations. Result: Animals were sacrificed at the conclusion of the study, and the organs were examined for histological alterations. Additionally, the biochemical factors such as calcium, oxalate, uric acid, protein, citrate, and magnesium that contribute to kidney stone formation were examined. To verify the drug's effectiveness and safety, tests on enzymes including lactate dehydrogenase and oxalate were also conducted. Significant information from the histopathology examinations and X-rays confirmed the effectiveness of corn silk and sour orange in urolithiasis. The results indicate that fraction extract of corn silk and sour orange shows significant antiurolithiatic activity. Conclusion: Corn silk and sour orange has shown very good efficacy and safety, hence safe for use in humans.
Cite this article:
Dighe Pooja, Vikhe Sunayana, Jadhav Ravindra, Rahul L. Vikhe. Phytochemical study and Pharmacological effect of corn silk and sour orange on ethylene glycol induced Urolithiasis in rats. Research Journal of Pharmacy and Technology. 2024; 17(10):4739-4. doi: 10.52711/0974-360X.2024.00730
Cite(Electronic):
Dighe Pooja, Vikhe Sunayana, Jadhav Ravindra, Rahul L. Vikhe. Phytochemical study and Pharmacological effect of corn silk and sour orange on ethylene glycol induced Urolithiasis in rats. Research Journal of Pharmacy and Technology. 2024; 17(10):4739-4. doi: 10.52711/0974-360X.2024.00730 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2024-17-10-15
11. REFERENCES:
1. Swati Sharma, Dileep Singh Baghel, Amit Mittal, Saurabh Singh, Bimlesh Kumar, Pranav Kumar Prabhakar, Anand Kumar Chaudhary. Polyherbal Formulation Development and Assessment of its Potential against Urolithiasis (Mutrakricchra) by In-Vitro Technique. Research Journal of Pharmacy and Technology. 2021; 14(4): 1982-8.
2. Aithamraju Satishchandra, M. Surya Teja, V. Sravani, M. Chinna Eshwariah. In vitro Urolithiasis activity of Thinopyrum intermedium methanolic extract on calcium oxalate crystals prepared by Precipitation method. Research Journal of Pharmacy and Technology. 2021; 14(3): 1310-1312.
3. A. B. D. Selvam. Can the term Phytosensology be preferred over the term Organoleptic. Pharmacognosy Res, 2010; 2(4): 271, doi: 10.4103/0974-8490.69131.
4. Y. Barghouthy and B. K. Somani. Role of Citrus Fruit Juices in Prevention of Kidney Stone Disease (KSD): A Narrative Review. Nutrients. 2021; 13(11): 4117 doi: 10.3390/nu13114117.
5. R. A. Bellanger and C. M. Seeger, “Complementary and alternative medicine. Side Effects of Drugs Annual. 2021; 493–502. doi: 10.1016/bs.seda.2021.07.003.
6. R. Lokhande, P. Singare, M. Andhele, and R. Acharya. Study of some Indian medicinal plants by application of INAA and AAS techniques. Nat Sci (Irvine). 2010; 2(1): 26–32, doi: 10.4236/ns.2010.21004.
7. S. R. Jarapala, G. Shivudu, K. Mangathya, A. Rathod, H. Panda, and P. K. Reddy. A New Approach for Identifying Potentially Effective Indigenous Plants Consumed by Chenchu Tribes and Their Nutritional Composition-India. Am J Plant Sci. 2021; 12(8): 1180–1196 doi: 10.4236/ajps.2021.128082.
8. R. Kalai, B. Sasirekha. Dietary Guidelines to Reduce the Risk of Renal Stones. Int. J. Nur. Edu. and Research. 2018; 6(1): 73-77.
9. World Health Organization. Quality control methods for herbal materials. World Health Organization, 2011.
10. A. Abubakar and M. Haque. Preparation of medicinal plants: Basic extraction and fractionation procedures for experimental purposes. J Pharm Bioallied Sci. 2020; 12(1). doi: 10.4103/jpbs.JPBS_175_19.
11. C. Sumanjali, M. Shashidhar, M. Sravani, K. Reddy Babu, B. Tejeswarudu, C. Deepthi Kalyani. Anti-Urolithiatic Activity of the Ethanolic Extract of Cassia auriculata against Ethylene Glycol Induced Urolithiasis in Experimental Rats. Research Journal of Pharmacy and Technology. 2021; 14(10):5207-2.
12. Anshuman Rai, Anamika Gautam, Sakshi Panchal, Ankita Sood, Pankaj Prashar, Narendra Kumar Pandey, Indu Melkani, Bimlesh Kumar. Protective effect of Ethanolic extract of Terminalia arjuna bark against Ethylene Glycol induced Urolithiasis in male rats: In-Vitro and In-Vivo Evaluation. Research J. Pharm. and Tech. 2020; 13(12):6132-6139.
13. F. Alam and Q. Najum us Saqib.Pharmacognostic standardization and preliminary phytochemical studies of Gaultheria trichophylla. Pharm Biol. 2015; 53(12): 1711–1718 doi: 10.3109/13880209.2014.1003355.
14. S. C. Gad and C. P. Chengelis. Acute Toxicology Testing. CRC Press. 1988. doi: 10.1201/9781439805213.
15. V. P., A. M. Khoviv, and A. Ju. Zavrazhnov. Physicochemical Analysis and Synthesis of Nonstoichiometric Solids. Materials Science - Advanced Topics, InTech. 2013. doi: 10.5772/54815.
16. Surya S, Priscilla Suresh. Antiurolithiatic Activity of Ethanolic Extract of Alpinia zerumbet Pers. Rhizome in the Adult Wistar Rats. Research Journal of Pharmacy and Technology. 2023; 16(7): 3379-4.
17. Vikhe S. Kunkulol R. Microscopic Investigations and Pharmacognosy of Striga orobanchioides Benth. Pharmacogn J. 2020; 12(6): 1325-31.
18. Chandrasekar R, R. Pavithra, B. Vandana, R. Babitha, S. Tejoprasanna, B. Sandeep, G. Deepika, M. Niranjan Babu. Evaluation of in vitro Antiurolithiatic Potential of Ethanolic Leaf Extract of Langerstromia specoisa. Research Journal of Pharmacognosy and Phytochemistry. 2022; 14(3): 150-4.
19. Sunayana Vikhe, Shweta Dhavane, Rajashree Ghogare. Formulation and Evaluation of Herbal Kajal for its Anti-Inflammatory, Anti-Microbial, Anti-Acne properties. Research Journal of Pharmacy and Technology 2023; 16(7): 3295-9. doi: 10.52711/0974-360X.2023.00543
20. OECD/OCDE 423 OECD Guideline for testing of chemicals Acute Oral Toxicity-Acute Toxic Class Method introduction, 2001.
21. Samir K. Shah, Kruti M. Patel, Pinal M. Vaviya. Evaluation of Antiurolithiatic activity of Betula utilis in Rats Using Ethylene Glycol Model. Asian J. Pharm. Res. 2017; 7(2): 81-87.
22. Vikhe S. Kunkulol R. Raut D. Antidiabetic and antihyperlipidemic effects of crude fractions and isolated compound from Striga orobanchioides Benth on streptozotocin induced diabetic rats. Journal of Ayurveda and Integrative Medicine. 2022; 13(3): 100618.
23. Sunayana Vikhe, Rahul Kunkulol, Dipak Raut. In Silico and In Vivo Studies of DecursinIsolated from the Ethanolic Extract of Feronia elephantum Correa (Rutaceae) Bark as a Potential Antidiabetic and Antihyperlipidemic Agent in STZ-induced Diabetic Rats. 2022; 20: 517-535.