Author(s):
Umesh Chaurasia, Vishal Soni, Ram Kumar Sahu
Email(s):
umeshc7@gmail.com
DOI:
10.52711/0974-360X.2024.00745 
Address:
Umesh Chaurasia1*, Vishal Soni1, Ram Kumar Sahu2
1B R Nahata College of Pharmacy (Faculty of Pharmacy), Mandsaur University, Mandsaur- 458001 (MP), India.
2Department of Pharmaceutical Sciences, Hemvati Nandan Bahuguna Garhwal University (A Central University), Chauras Campus, Tehri Garhwal-249161, Uttarakhand, India.
*Corresponding Author
Published In:
Volume - 17,
Issue - 10,
Year - 2024
ABSTRACT:
Gallic acid and quercetin are the flavonoids present in the Cassia tora and play significant role in healing number of ailments. No method has yet been developed to separate and identify gallic acid and quercetin from Cassia tora extract using thin layer chromatography (TLC). The TLC method is simple and affordable for the identification of flavonoids compared to advanced chromatography tools. Therefore, the aim was to create a TLC-based method for identifying and isolating gallic acid and quercetin from the Cassia tora extract. The petroleum ether and hydroalcoholic extract were prepared form the aerial part of the Cassia tora. The petroleum ether and hydroalcoholic extract were subjected to the phytochemical analysis. The TLC was performed with hydroalcoholic extract to separate and identify gallic acid and also quercetin. Mobile phase made in ratio (4:5:1) and (5:7:1), from reagents i.e. ethyl acetate, toluene, and formic acid, was used for the separation of quercetin and gallic acid, respectively. The findings of phytochemical studies expressed existence of polyphenol and flavonoids in extract with hydro-alcohol, so that hydroalcoholic extract was used for TLC study. The extract and pure quercetin exhibited 0.62 value of Rf, while extract and gallic acid revealed 0.34 Rf value, authorizing existence of quercetin and also gallic acid in Cassia tora extract. This analysis thus concluded that TLC is most effective approach for identifying and isolating components i.e. gallic acid and also quercetin from extract of Cassia tora.
Cite this article:
Umesh Chaurasia, Vishal Soni, Ram Kumar Sahu. Separation of Gallic acid and Quercetin from the Cassia tora extract Using Thin Layer Chromatography: Simple and Cost-Effective Approach. Research Journal of Pharmacy and Technology. 2024; 17(10):4847-0. doi: 10.52711/0974-360X.2024.00745
Cite(Electronic):
Umesh Chaurasia, Vishal Soni, Ram Kumar Sahu. Separation of Gallic acid and Quercetin from the Cassia tora extract Using Thin Layer Chromatography: Simple and Cost-Effective Approach. Research Journal of Pharmacy and Technology. 2024; 17(10):4847-0. doi: 10.52711/0974-360X.2024.00745 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2024-17-10-30
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