Author(s):
Tengku Ismanelly Hanum, Hetty Lendora Maha, Henny Sri Wahyuni, Rahmad Fadli, Indah Yuliasari Saragih
Email(s):
hetty_maha03@usu.ac.id
DOI:
10.52711/0974-360X.2024.00721 
Address:
Tengku Ismanelly Hanum1,2, Hetty Lendora Maha1*, Henny Sri Wahyuni3, Rahmad Fadli1, Indah Yuliasari Saragih1
1Department of Pharmaceutical Technology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, 20155, Indonesia.
2Nanomedicine Innovation Center, Universitas Sumatera Utara, Medan, 20155, Indonesia.
3Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, 20155, Indonesia.
*Corresponding Author
Published In:
Volume - 17,
Issue - 10,
Year - 2024
ABSTRACT:
Kersen (Muntingiaa calabura L.) is one of the plants empirically used by the public as an anti-inflammatory drug. Muntingiaa calabura is a flowering plant belonging to the Elaocarpaceae family. Kersen contains flavonoids, saponins, and tannins. The flavonoids that are contained in Kersen are flavones, flavanones, flavanones, and biflavan. Flavonoids have received much attention because of their antibacterial, anti-inflammatory, and antioxidant properties. Nanotechnology is intended to increase the capability activities of Kersen leaves phytochemical compounds. In nano size, the surface contact area of particles becomes more extensive, which can increase the number of active substances isolated, thus increasing anti-inflammatory activity. This study aimed to prepare and determine the anti-inflammatory activity of nanoparticles of ethanol extract kersen leaves (NEEKL) (Muntingia calabura L.). Preparation of NEEKL by ionic gelation method using variations of chitosan and sodium tripolyphosphate (TPP-S), then characterized for its particle size and morphology. The anti-inflammatory activity of NEEKL was evaluated using the carrageenan-induced rat paw edema method. It was performed in six different groups. Each group consisted of 4 rats. The negative control was given 0.5% CMC-Na suspension; the positive control was given diclofenac sodium 4.5mg/kg body weight; the 3rd and 4th were given NEEKL as much 100, 300mg/kg body weight; 5th groups were given ethanolic extract kersen leaves (EEKL), and 6th group was given 1% chitosan-TPP-S. The measurements were done in 6 hours with intervals of 60 minutes. All the data obtained were statistically analyzed. The NEEKL with variation chitosan: TPP-S (1.5:1) had the smallest particle size (94.21nm). Based on the percentage of inflammation, there was a significant difference between negative control and no significant difference between positive control and other groups. The percentage of anti-inflammatory activity of positive control, NEEKL (100mg/kg body weight), NEEKL (300mg/kg body weight), EEKL (500mg/kg body weight), and chitosan-TPPS complex were 86.10±0.90%; 81.16±0.55%; 84.47±0.85%; 85.51±1.56%, and 77.44±1.83% respectively. EEKL can be made in the form of nanoparticles using the ionic gelation method to fulfill the nanoparticle requirements. NEEKL has effective anti-inflammatory activity at a dose of 300mg/kg body weight.
Cite this article:
Tengku Ismanelly Hanum, Hetty Lendora Maha, Henny Sri Wahyuni, Rahmad Fadli, Indah Yuliasari Saragih. Nanoparticles of Muntingia calabura L. Ethanol extract: Preparation and Anti-inflammatory activity. Research Journal of Pharmacy and Technology. 2024; 17(10):4677-4. doi: 10.52711/0974-360X.2024.00721
Cite(Electronic):
Tengku Ismanelly Hanum, Hetty Lendora Maha, Henny Sri Wahyuni, Rahmad Fadli, Indah Yuliasari Saragih. Nanoparticles of Muntingia calabura L. Ethanol extract: Preparation and Anti-inflammatory activity. Research Journal of Pharmacy and Technology. 2024; 17(10):4677-4. doi: 10.52711/0974-360X.2024.00721 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2024-17-10-6
REFERENCES:
1. Anbarasi, A., and Vidhya, R. Evaluation of In Vitro Anti-Inflammatory Activity of Tephrosia purpurea (Seed). Asian J. Pharm. Res. 2015; 5(2): 83–89.
2. Mycek MJ, Harvey RA, and Champe PC. Pharmacology Illustrated Review. 2001; 2nd Eds, Medical Book EGC, Jakarta: 404-413.
3. Rupesh Pandey, Ravindra Pandey, SS Shukla. Anti-inflammatory potential of ethanol extract of Rubus ulmifolius (Schott). Research J. Pharm. and Tech. 2013; 6(3): 300-303.
4. Lalitha, P., Sachithanandam, V., Swarnakumar, N. S., and Sridhar, R. Review on Anti-inflammatory Properties of Mangrove Plants. Asian J. Pharm. Res. 2019; 9(4): 273–288.
5. Kulwinder Singh, Monika, Neelam Verma. Gastrointestinal and Cardiovascular Risks of Non-Steroidal Anti-Inflammatory Drugs (NSAIDs): Time to Translate Knowledge into Practice. Research J. Pharm. and Tech. 2014; 7(5): 575-580.
6. Furst DE, Urlich RW, and Prakash S. Non-steroidal Anti-inflammatory, Antirheumatic, Disease-Modifying, Non-opioid Analgetic, and for Gout, in: B.G. Katzung, Basic and Clinical Pharmacology. 2013; 12th Ed Vol. 2, EGC, Jakarta.
7. Liling, S., Roska, T. P., Arfiansyah, R., Maryam, F., and Nainu, F. Pharmacological effect of muntingia calabura leaves on the expression of sod1 and sod2 in Drosophila. Biointerface Research in Applied Chemistry. 2021; 11(5): 12985–12992. doi: 10.33263/BRIAC115.1298512992.
8. Narapusetty, N., Sivaiah, O., Balanasaraiah, B., Haranadhbabu, M., and Prasad, B. Anti-Inflammatory activity of Ethanolic extract of Basella alba in acute and Sub-acute Model. Asian J. Pharm. Res. 2017; 7(2): 88–93.
9. Aravind R., Bindu A.R., Bindu K., Kanthlal S., AnilKumar B. Anti-Inflammatory Evaluation of the Cinnamomum malabatrum (Burm. F). Blume Leaves using Carrageenan Induced Rat Paw Oedema Method. Research J. Pharm. and Tech. 2013; 6(7): 746-748.
10. Mahmood ND, Nasir NL, Rofiee MS, Tohid SF, Ching SM, Teh LK, Salleh MZ, Zakaria ZA. Muntingia calabura: a review of its traditional uses, chemical properties, and pharmacological observations. Pharm Biol. 2014; 52(12): 1598-623. doi: 10.3109/13880209.2014.908397.
11. Kuo WL, Liao HR, Chen JJ. Biflavans, Flavonoids, and a Dihydrochalcone from the Stem Wood of Muntingia calabura and Their Inhibitory Activities on Neutrophil Pro-Inflammatory Responses. Molecules. 2014; 19(12): 20521-20535. doi: 10.3390/molecules191220521.
12. Buzea C, Blandino IIP, and Robbie K. Nanomaterials and Nanoparticles: Sources and Toxicity. Biointerphases. 2007; 2(4): 17-172.
13. Kammona O, Kiparissides C. Recent advances in nanocarrier-based mucosal delivery of biomolecules. J Control Release. 2012; 161(3): 781-94. doi: 10.1016/j.jconrel.2012.05.040.
14. Ariyanta HA, Wahyuni S, and Priatmoko S. Preparation of Silver Nanoparticles by Reduction Method and Their Application as Antibacterials for Infectious Causes. Indo.J.Che, Sci. 2014; 3(1): 2-5.
15. Balaji and Selvan. Nanopharmacology: A Novel Approach In Therapeutics Introduction. Asian J. Pharm. Res. 2019; 9(1): 9–16.
16. G.O. Birajdar, V.S. Kadam, A.G. Chintale, P.D. Halle, M.K. Nabde, K.S. Maske. A Comprehensive Review on Nanotechnology. Research J. Pharm. and Tech. 2013; 6(5): 486-495.
17. Abubakar AR, Haque M. Preparation of Medicinal Plants: Basic Extraction and Fractionation Procedures for Experimental Purposes. J Pharm Bioallied Sci. 2020; 12(1): 1-10. doi: 10.4103/jpbs.JPBS_175_19.
18. Subhashis Debnath, R. Suresh Kumar, M. Niranjan Babu. Ionotropic Gelation – A Novel Method to Prepare Chitosan Nanoparticles. Research J. Pharm. and Tech. 2011; 4(4): 492-495.
19. Venkateswara Rao, D. Bhagya Sri Vani, K. Padmalatha. Development and Characterization of Chitosan-based Flutamide Nanoparticles by Ionic Gelation Method. Research J. Pharm. and Tech. 2021; 14(3): 1668-1672. doi: 10.5958/0974-360X.2021.00296.1.
20. John I D’souza, Harinath N More. Topical Anti-Inflammatory Gels of Fluocinolone Acetonide Entrapped in Eudragit Based Microsponge Delivery System. Research J. Pharm. and Tech. 2008; 1(4): 502-506.
21. Purushothaman, Sowjanya Battu, K. Jyothshna Devi, C. Madhusudhana Chetty, M. Alagusundaram, K. Mallikarjuna Rao. Formulation and Characterization of Ofloxacin Microspheres Prepared by Ionotropic Gelation Technique. Research J. Pharm. and Tech. 2010; 3(4): 1265-1269.
22. Department of Health of the Republic of Indonesia. Materia Medika Indonesia. 6th edition. 1995. Jakarta. 321; 324-325: 333-334: 336.
23. Manpreet Kaur. Evaluation of Anti-inflammatory Activity of Ethanol Extract of Bark of Aspidosperma quebracho-blanco. Research J. Pharm. and Tech. 2013; 6(5): 575-576.
24. Shinde, P. P., Khule, S. D., Sonawane, S., and Shelke, S. Analgesic activity and anti-inflammatory activity of methanolic extract of plant Sida cordata in carrageenan-induced paw edema in rats. Asian J. Pharm. Res. 2021; 11(3): 143–146.
25. VH Bhaskar, B Sangameswaran, N Balakrishnan, AB Panda, Navin R Raj, A Sathish. Screening of Analgesic and Anti-inflammatory Activity of Hydroalcohol Extract of Sida (Indian) Species Root. Research J. Pharm. and Tech. 2008; 1(3): 287-289.
26. Handani, W. R., Sediawan, W. B., Tawfiequrrahman, A., Wiratni, and Kusumastuti, Y. The effect of temperature and chitosan concentration during storage on the growth of chitosan nanoparticles produced by ionic gelation. AIP Conference Proceedings. 2017; 1840(5). doi:10.1063/1.4982299.
27. Sawtarie, N., Cai, Y., and Lapitsky, Y. Preparation of chitosan/tripolyphosphate nanoparticles with highly tunable size and low polydispersity. Colloids and Surfaces B: Biointerfaces. 2017; 157: 110–117. doi: 10.1016/j.colsurfb.2017.05.055.
28. Van Bavel, N., Issler, T., Pang, L., Anikovskiy, M., and Prenner, E. J. A Simple Method for Synthesis of Chitosan Nanoparticles with Ionic Gelation and Homogenization. Molecules. 2-23; 28(11): 1–14. doi: 10.3390/molecules28114328
29. Malviya, K. G., and Shivhare, S. C. Evaluation of Anti-inflammatory Potential of Cyathocline lyrata Cass Plant Extract by using Carrageenan Induced and Formalin Induced Rat Paw. Asian J. Pharm. 2013; 3(2): 90–94.
30. Zakaria ZA, Mahmood ND, Omar MH, Taher M, Basir R. Methanol extract of Muntingia calabura leaves attenuates CCl4-induced liver injury: possible synergistic action of flavonoids and volatile bioactive compounds on endogenous defence system. Pharm Biol. 2019; 57(1): 335-344. doi: 10.1080/13880209.2019.1606836.
31. Goli, V., Bhaskar, K. V., Bhaskar, J., Devi, P. S., and Ramchander, T. Effects of Anti-Inflammatory Activity of Mimosa pudica. Asian J. Pharm. Res. 2011; 1(3): 69–71.
32. Vinay, C. H., Goudanavar, P., Acharya, A., Ahmed, M. G., and R, P. K. S. Development and Characterization of Orange Peel Extract Based Nanoparticles. Asian J. Pharm. Res. 2018; 8(2): 71–77.
33. Mansjoer S. Mechanism of Anti-Inflammatory Drugs. Indonesian Pharmaceutical Media. 2003; 7(1): 35-36.
34. Sarika V. Khandbahale, R. B. Saudagar. Nanoparticle- A Review. Asian J. Res. Pharm. Sci. 2017; 7(3): 162-172. doi: 10.5958/2231-5659.2017.00026.1
35. Patil, M. O., Patil, P. A., and Karnavat, D. R. Development of Immunotherapeutic Nanoparticles for treatment of Tuberculosis. Asian J. Pharm. 2020; 10(3): 226–232.
36. Singh R, Lillard JW Jr. Nanoparticle-based targeted drug delivery. Exp Mol Pathol. 2009; 86(3): 215-23. doi: 10.1016/j.yexmp.2008.12.004.