Author(s): Shribhumika L, Ramgopal M Dhanwad

Email(s): ramgopal.dhanwad@gmail.com

DOI: 10.52711/0974-360X.2024.00167   

Address: Shribhumika L, Ramgopal M Dhanwad
Government College of Pharmacy, Department of Pharmaceutical Chemistry, Bengaluru, Karnataka.
*Corresponding Author

Published In:   Volume - 17,      Issue - 3,     Year - 2024


ABSTRACT:
A new, simple, accurate, and economical method was developed for simultaneous estimation of Lamivudine and Raltegravir by RP-HPLC. The chromatographic separation was achieved through Phenomenex C18 250mm × 4.6mm, 5µm as stationary phase and mobile phase was Methanol: Acetonitrile: Millipore Water (60:10:30) v/v ratio with the flow rate of 1ml/min at 292nm. The developed method was validated for the quantitative analysis of commercially available pure drugs. The instrument used was Shimadzu PDA-20AR, Lab solution software. The retention time for Lamivudine and Raltegravir were found to be 2.883 and 4.430 respectively. The linearity study of Lamivudine was performed by using concentration range of 1-20µg/ml and for Raltegravir concentration range was at 10-30µg/ml and correlation coefficient (r2) was found to be 0.999 and 0.9977 respectively. The developed method was validated in reference to ICH Guidelines. Specificity, Linearity, Accuracy, Robustness, Precision and Ruggedness obtained within the acceptance range.


Cite this article:
Shribhumika L, Ramgopal M Dhanwad. Method Development and Validation for Simultaneous Estimation of Lamivudine and Raltegravir in Pure Drug form by RP-HPLC. Research Journal of Pharmacy and Technology. 2024; 17(3):1071-5. doi: 10.52711/0974-360X.2024.00167

Cite(Electronic):
Shribhumika L, Ramgopal M Dhanwad. Method Development and Validation for Simultaneous Estimation of Lamivudine and Raltegravir in Pure Drug form by RP-HPLC. Research Journal of Pharmacy and Technology. 2024; 17(3):1071-5. doi: 10.52711/0974-360X.2024.00167   Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2024-17-3-18


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