Author(s): Natarajan K, Nisha Shri C, Kumaresh Jawahar N, Niranjan Shakthi M


DOI: 10.52711/0974-360X.2024.00184   

Address: Natarajan K1, Nisha Shri C2, Kumaresh Jawahar N3, Niranjan Shakthi M4
1Professor, Department of Pharmaceutics, T John College of Pharmacy, Bangalore.
2Associate Professor, Department of Pharmacology, T John College of Pharmacy, Bangalore.
3Scholar, Department of Biotechnology, SASTRA University, Thanjavur.
4Scholar, Department of Biotechnology, T John College of Pharmacy, Bangalore.
*Corresponding Author

Published In:   Volume - 17,      Issue - 3,     Year - 2024

Objective: Mucuna cochinchinensis (Fabaceae)is a widely grown twiner that has been used in the traditional medicine for treating many ailments. The objective of the present study was to evaluate the hypoglycemic effects of ethyl acetate extract of M. cochinchinensis seeds on alloxan-induced diabetic rat models. Materials and Methods: M.cochinchinensis seeds were collected and macerated with ethyl acetate to obtain ethyl acetate extract of M. cochinchinensis (EMC). The obtained extract was subjected to preliminary phytochemical analysis using standard protocol and HPLC-MS study to identify the phytoconstituents present. Effect of EMC on parameters like blood glucose and plasma insulin levels were assessed using commercial kits in alloxan-induced experimental rats. Results: Preliminary phytochemical analysis of EMC showed the presence of alkaloids, flavonoids, terpenoids, saponins, tannins and glycosides. HPLC-MS study revealed the presence of 6-C-Pentosyl-8-C-hexosyl apigenin and kaempferol-3-O-rutinoside in EMC. EMC effect on alloxan-induced diabetic ratsblood glucose level on 1st, 7th and 14th day and plasma insulin levels were determined and was found to revert the blood glucose levels significantly (p<0.01). Conclusion: To conclude, EMC has potent efficacy in normalizing blood glucose and serum insulin concentration in alloxan induced diabetic rats.

Cite this article:
Natarajan K, Nisha Shri C, Kumaresh Jawahar N, Niranjan Shakthi M. Effect of Mucuna cochinchinensis seed extract on Alloxan-Induced Diabetic Experimental Rats. Research Journal of Pharmacy and Technology. 2024; 17(3):1185-9. doi: 10.52711/0974-360X.2024.00184

Natarajan K, Nisha Shri C, Kumaresh Jawahar N, Niranjan Shakthi M. Effect of Mucuna cochinchinensis seed extract on Alloxan-Induced Diabetic Experimental Rats. Research Journal of Pharmacy and Technology. 2024; 17(3):1185-9. doi: 10.52711/0974-360X.2024.00184   Available on:

1.    Amita Shobha Rao, Shobha K L, Preethi Lavina Concessao, Gowrish Rao S, Sreedhara R Pai K. In vitro Evaluation of the Antimicrobial activity of Methanolic and Aqueous extract of Mucuna pruriens seed. Research Journal of Pharmacy and Technology. 2021; 14(8):4212-4. doi: 10.52711/0974-360X.2021.00730.
2.    Soumya Menon, Happy Agarwal, S. Rajeshkumar, S. Venkat Kumar. Anticancer assessment of biosynthesized silver nanoparticles using Mucuna pruriens seed extract on Lung Cancer Treatment. Research Journal of Pharmacy and Technology. 2018; 11(9): 3887-3891. doi: 10.5958/0974-360X.2018.00712.6.
3.    Sridhar KR, Bhat R. Agrobotanical, nutritional and bioactive potential of unconventional legume–Mucuna. Livestock Research for Rural Development. 2007; 19(9):126-30.
4.    Pathania R, Chawla P, Khan H, Kaushik R, Khan MA. An assessment of potential nutritive and medicinal properties of Mucuna pruriens: a natural food legume. 3 Biotech. 2020; 10(6): 261. doi: 10.1007/s13205-020-02253-x.
5.    Natarajan K, Narayanan N, Natesan Ravichandran, Lalitha K.G. Antioxidant and anticancer activity of Mucuna cochinchinensis (Lour.) Cheval seeds. International Journal of Pharmacy and Pharmaceutical Sciences. 2013; 5:646-648.
6.    Handajani S. Indigenous mucuna tempe as functional food. Asia Pacific Journal of Clinical Nutrition. 2001; 10(3): 222-5. doi: 10.1046/j.1440-6047.2001.00243.x.
7.    Maphosa Y, Jideani VA. The role of legumes in human nutrition, Functional Food-Improve Health through Adequate Food. InTechOpen. doi:10.5772/intechopen.69127
8.    Monika Sahu, Vinod Kumar, Veenu Joshi. Indian medicinal plants with antidiabetic potential: An overview. Research Journal of Pharmacy and Technology. 2021; 14(4):2328-5. doi: 10.52711/0974-360X.2021.00411.
9.    Alimuddin Saifi, Rajani Chauhan, Jaya Dwivedi. Development of a polyherbal formulation FMST and evaluation for antidiabetic activity in alloxan induced diabetic rats. Asian Journal of Pharmaceutical Research. 2017; 7(1): 1-7. doi: 10.5958/2231-5691.2017.00001.6.
10.    K. Kishore Kumar, R.S.K. Sharma, P. ChantiBabu, M. Sreenivasa Rao, K. DurgaPrasadu, D. Ravi Kumar. Synthesis, characterization and pharmacological evaluation of novel spiro heterocyclic compounds as anti-diabetic agents. Asian Journal of Research in Chemistry. 2017; 10(3): 393-398. doi: 10.5958/0974-4150.2017.00067.0
11.    Dongare SS, Maske AP, Patil SM, UmbareRP, Mate GS. Antidiabetic Activity of Marsileaquadrifolialinn in Alloxan-Diabetic Rats. Research Journal of Pharmacognosy and Phytochemistry. 2009; 1(1):15-17.
12.    Krushna H Pande, Pramod S Salve, Raghvendra K Rai, Nikhil R Bali, Harshad S Wankhede. Investigation and In vivo Demonstration of Synergistic Antidiabetic activity of Aqueous Extract of Aeglemarmelos and Withaniacoagulans. Research Journal of Pharmacology and Pharmacodynamics. 2014; 6(2): 101-107.
13.    Nadiminti Satish Reddy, Vidyasabbani, B. Pravanthi, B. Vijaya Laxmi, B. Harika. Evaluation of Antidiabetic Activity of Rumex vesicarius in Streptozotocin Induced Diabetic Albino Rats. Research Journal of Pharmacology and Pharmacodynamics. 2016; 8(3): 123-126. doi: 10.5958/2321-5836.2016.00023.9
14.    Lokhande V.Y, Yadav A.V. In vitro Antioxidant and Antidiabetic Activity of Supercritical Fluid extract of Leaves Ocimum sanctum L. Research Journal of Pharmacy and Technology. 2018; 11(12): 5376-5378. doi: 10.5958/0974-360X.2018.00980.0.
15.    Yuvaraja K R, Santhiagu A, Jasemine S, Gopalasathees Kumar K. Hepatoprotective activity of Chloroform and Ethyl acetate extract of Dipteracanthus patulus against Paracetamol induced Hepatotoxicity in rats through Antioxidant mechanism. Research Journal of Pharmacy and Technology. 2020; 13(1):203-208. doi: 10.5958/0974-360X.2020.00041.4.
16.    Novena Yety Lindawati, Dian Puspitasari, Lusia Murtisiwi, Tesia Aisyah Rahmania. Correlation of Flavonoid content on Antidiabetic activity in red beans (Phaseulus vulgaris L.) and its Processed Products. Research Journal of Pharmacy and Technology. 2021; 14(3):1293-1297. doi: 10.5958/0974-360X.2021.00229.8.
17.    Harborne J. B. 2005. Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis. 3rd edition. Springer (India) Pvt. 2005, New Delhi: pp 75-89.
18.    Kokate CK, Purohit AP, Gokhale SB. Pharmacognosy, NiraliPrakashan. Page no. 2005:1-6.
19.    Natarajan K, Narayanan N, Ravichandran N, Chidambaranathan N.Effect of Mucuna cochinchinensis seed extract on 7, 12-dimethyl benzanthracene induced mammary carcinoma. Innovare Journal of Health Sciences. 2016; 4(3):4-8.
20.    E E Bolton, Y Wang, P A Thiessen, S H Bryant.Chapter 12 PubChem: Integrated Platform of Small Molecules and Biological Activities. Annual Reports in Computational Chemistry. 2008; 4: 217-241. doi:10.1016/S1574-1400(08)00012-1
21.    Rohilla A, Ali S. Alloxan induced diabetes: mechanisms and effects. International journal of research in pharmaceutical and biomedical sciences. 2012; 3(2):819-23.
22.    R Sathish, K Natarajan, S Selvakumar. Antidiabetic Activity of Swietenia mahagoni Seed Powder in Alloxan Induced Diabetic Mice. Research Journal of Pharmacology and Pharmacodynamics. 2010; 2(4): 296-299.
23.    Alimuddin Saifi, Rajani Chauhan, Jaya Dwivedi. Assessment of the antidiabetic activity of Syzygium cumini (Linn.) Skeels in alloxan induced diabetic rats. Research Journal of Pharmacology and Pharmacodynamics.2016; 8(3): 91-96. doi: 10.5958/2321-5836.2016.00017.3.
24.    Zhang L, Xu L, Xiao SS, Liao QF, Li Q, Liang J,et al.Characterization of flavonoids in the extract of Sophora flavescens Ait. by high-performance liquid chromatography coupled with diode-array detector and electrospray ionization mass spectrometry. Journal of Pharmaceutical and Biomedical Analysis. 2007; 44(5):1019-28. doi: 10.1016/j.jpba.2007.04.019.
25.    Annapurna HV, Apoorva B, Ravichandran N, Arun KP, Brindha P, Swaminathan S, et al.Isolation and in silico evaluation of antidiabetic molecules of Cynodon dactylon (L.). Journal of Molecular Graphics and Modelling. 2013; 39:87-97. doi: 10.1016/j.jmgm.2012.10.009.
26.    Sandra Silva, Ana A. Matias, Ana Nunes, Catarina Duarte, M. Rosário Bronze, Ana V. Coelho. Identification of flavonol glycosides in winemaking by-products by HPLC with different detectors and hyphenated with mass spectrometry. CiênciaTéc. Vitiv. 2005;20(1):17-33
27.    Natarajan K, et al.Toxicity studies on ethyl acetate extract of Mucuna cochinchinensis seed. The International Journal of Advanced Research in Pharmaceutical and Bio Sciences. 2018; 8(3): 1-7
28.    Ighodaro OM, Adeosun AM, Akinloye OA. Alloxan-induced diabetes, a common model for evaluating the glycemic-control potential of therapeutic compounds and plants extracts in experimental studies. Medicina (Kaunas). 2017; 53(6):365-374. doi: 10.1016/j.medici.2018.02.001.
29.    Lenzen S. The mechanisms of alloxan- and streptozotocin-induced diabetes. Diabetologia. 2008; 51(2):216-26. doi: 10.1007/s00125-007-0886-7.
30.    Rodrigues PV, Lemos BMS, Silva MVD, de Campos Lima T, Santos DO, Lemes JBP, et al. Alloxan as a better option than streptozotocin for studies involving painful diabetic neuropathy. Journal of Pharmacological and Toxicological Methods. 2021; 112:107090. doi: 10.1016/j.vascn.2021.107090.
31.    Kashyap B, Saikia K, Samanta SK, Thakur D, Banerjee SK, Borah JC, et al. Kaempferol 3-O-rutinoside from Antidesma acidum Retz. Stimulates glucose uptake through SIRT1 induction followed by GLUT4 translocation in skeletal muscle L6 cells. Journal of Ethnopharmacology. 2023; 301:115788. doi: 10.1016/j.jep.2022.115788.
32.    Calderón-Montaño JM, Burgos-Morón E, Pérez-Guerrero C, López-Lázaro M. A review on the dietary flavonoid kaempferol. Mini reviews in medicinal chemistry. 2011; 11(4):298-344. doi: 10.2174/138955711795305335.
33.    Habtemariam S. α-Glucosidase inhibitory activity of kaempferol-3-O-rutinoside. Natural Product Communications. 2011; 6(2):201-3.
34.    Vargas E, Joy NV, Carrillo Sepulveda MA. Biochemistry, Insulin Metabolic Effects. [Updated 2022 Sep 26]. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2023 Jan-. Available from:
35.    Vazquez Encalada S, Segura Campos MR. Mucunapruriensfiber: nutritional, functional and biological properties. Food Science and Technology. 2020; 41:120-6. doi:
36.    Okafor SN, Ezeokonkwo MA. Promixate Composition and Mineral Analysis of Mucunautilis (Velvet Bean). IOSR Journal of Applied Chemistry. 2015; 8(10):42-45.
37.    Jovanovski E, Khayyat R, Zurbau A, Komishon A, Mazhar N, Sievenpiper JL, et al. Should viscous fiber supplements be considered in diabetes control? Results from a systematic review and meta-analysis of randomized controlled trials. Diabetes Care. 2019; 42(5):755-66. doi: 10.2337/dc18-1126.
38.    Jovanovski E, Mazhar N, Komishon A, Khayyat R, Li D, Blanco Mejia S,et al. Effect of viscous fiber supplementation on obesity indicators in individuals consuming calorie-restricted diets: A systematic review and meta-analysis of randomized controlled trials. European journal of nutrition. 2021; 60:101-12. doi: 10.1007/s00394-020-02224-1.
39.    Bhaskar A, Vidhya VG, Ramya M. Hypoglycemic effect of Mucuna pruriens seed extract on normal and streptozotocin-diabetic rats. Fitoterapia. 2008;79(7-8):539-43. doi: 10.1016/j.fitote.2008.05.008.
40.    R Rajesh, Singh S, Anandraj V K, Kumarappan M,et al. The Effect of Mucunapruriens Seed Extract on Pancreas and Liver of Diabetic Wistar Rats. International Journal of Current Research and Review. 2016 Feb 15; 8(4):61.
41.    Rathi SS, Grover JK, Vats V. The effect of Momordica charantia and Mucuna pruriens in experimental diabetes and their effect on key metabolic enzymes involved in carbohydrate metabolism. Phytotherapy research. 2002; 16(3):236-43. doi: 10.1002/ptr.842.

Recomonded Articles:

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

56th percentile
Powered by  Scopus

SCImago Journal & Country Rank

Recent Articles


Not Available