Author(s): Yazan Sultan Altinawe, Ola Mahmoud Younes

Email(s): yazan.altinawe1998@damascusuniversity.edu.sy , Ola31.Younes@damascusuniversity.edu.sy.

DOI: 10.52711/0974-360X.2024.00190   

Address: Yazan Sultan Altinawe*, Ola Mahmoud Younes
Department of Analytical and Food Chemistry, Faculty of Pharmacy, Damascus University, Damascus, Syria.
*Corresponding Author

Published In:   Volume - 17,      Issue - 3,     Year - 2024


ABSTRACT:
Background: Minimizing the amount of organic solvents is one of the main goals of green analytical chemistry; Therefore, a rapid, simple, sensitive, and environmentally benign method for the determination of ranolazine in bulk and pharmaceuticaldosage form was developed. Methods: The method based on using ethanol s a green solvent: (4mmol/L) sodium sulfobutyl ether-betacyclodextrin SBE-ß-CD(pH in aqueous phase=4) (25:75)as a green mobile phase, C18 5?? (4.6*150mm) InertSustain GL Sciences column at room temperature 35°C, the flow rate was 1mL/min, the photo diode array detector was set at 210nm and injection volume was 20µL. Results: The developed method was validated according to International Conference on Harmonization guidelines and showed good linear relationship over the concentration range of 50-150mg/L with the correlation coefficient was obtained R2 = 0.9999. The LOD and LOQ were found to be 1.91 and 5.81mg/L, respectively. The Precision was studied as intra- and inter-day with relative standard deviations not more than 2%, and the accuracy by mean recoveries ranged between100.1-101%. The percentage recoveries of active pharmaceutical ingredient from dosage forms ranged between 99.67 - 100.2%. Addition of cyclodexetrine derivative to the mobile phase, decreases the retention time tR of the compound by 64%, more over, it allowed the method to be greener by the ability to use high percentage of aqueous phase. GAPI and eco-scale were used to evaluate the greenness of the developed method. Conclusion: The results showed that the developed eco-friendly method is suitable for the precise, accurate and rapid determination of Ranolazine in bulk and tablet dosage forms.


Cite this article:
Yazan Sultan Altinawe, Ola Mahmoud Younes. Eco-friendly HPLC Method for the determination of Ranolazine using Cyclodextrin as a green additive to mobile phase. Research Journal of Pharmacy and Technology. 2024; 17(3):1217-3. doi: 10.52711/0974-360X.2024.00190

Cite(Electronic):
Yazan Sultan Altinawe, Ola Mahmoud Younes. Eco-friendly HPLC Method for the determination of Ranolazine using Cyclodextrin as a green additive to mobile phase. Research Journal of Pharmacy and Technology. 2024; 17(3):1217-3. doi: 10.52711/0974-360X.2024.00190   Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2024-17-3-41


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