Author(s): Yazan Sultan Altinawe, Ola Mahmoud Younes

Email(s): ,

DOI: 10.52711/0974-360X.2024.00190   

Address: Yazan Sultan Altinawe*, Ola Mahmoud Younes
Department of Analytical and Food Chemistry, Faculty of Pharmacy, Damascus University, Damascus, Syria.
*Corresponding Author

Published In:   Volume - 17,      Issue - 3,     Year - 2024

Background: Minimizing the amount of organic solvents is one of the main goals of green analytical chemistry; Therefore, a rapid, simple, sensitive, and environmentally benign method for the determination of ranolazine in bulk and pharmaceuticaldosage form was developed. Methods: The method based on using ethanol s a green solvent: (4mmol/L) sodium sulfobutyl ether-betacyclodextrin SBE-ß-CD(pH in aqueous phase=4) (25:75)as a green mobile phase, C18 5?? (4.6*150mm) InertSustain GL Sciences column at room temperature 35°C, the flow rate was 1mL/min, the photo diode array detector was set at 210nm and injection volume was 20µL. Results: The developed method was validated according to International Conference on Harmonization guidelines and showed good linear relationship over the concentration range of 50-150mg/L with the correlation coefficient was obtained R2 = 0.9999. The LOD and LOQ were found to be 1.91 and 5.81mg/L, respectively. The Precision was studied as intra- and inter-day with relative standard deviations not more than 2%, and the accuracy by mean recoveries ranged between100.1-101%. The percentage recoveries of active pharmaceutical ingredient from dosage forms ranged between 99.67 - 100.2%. Addition of cyclodexetrine derivative to the mobile phase, decreases the retention time tR of the compound by 64%, more over, it allowed the method to be greener by the ability to use high percentage of aqueous phase. GAPI and eco-scale were used to evaluate the greenness of the developed method. Conclusion: The results showed that the developed eco-friendly method is suitable for the precise, accurate and rapid determination of Ranolazine in bulk and tablet dosage forms.

Cite this article:
Yazan Sultan Altinawe, Ola Mahmoud Younes. Eco-friendly HPLC Method for the determination of Ranolazine using Cyclodextrin as a green additive to mobile phase. Research Journal of Pharmacy and Technology. 2024; 17(3):1217-3. doi: 10.52711/0974-360X.2024.00190

Yazan Sultan Altinawe, Ola Mahmoud Younes. Eco-friendly HPLC Method for the determination of Ranolazine using Cyclodextrin as a green additive to mobile phase. Research Journal of Pharmacy and Technology. 2024; 17(3):1217-3. doi: 10.52711/0974-360X.2024.00190   Available on:

1.    Bawankar DL, Deshmane SV, More SM, Channawar MA, Chandewar AV, Shreekanth J.. Design and Characterization of Extended Release Ranolazine Matrix Tablet. Research J. Pharm. and Tech. 2009; 2 (4): 756-761..
2.    Patel Vishakha. D., Raj Hasumati. Ranolazine: A Review on Analytical Method and Its Determination in Synthetic Mixture. Asian J. Pharm. Ana. 2015; 5(4): 214-218. DOI : 10.5958/2231-5675.2015.00034.4.
3.    Vishakha D. Patel, Hasumati A. Raj, Nirav K. Gheewala. Second Derivative Spectroscopic Method for Simultaneous estimation of Amiodarone Hydrochloride and Ranolazine in synthetic mixture. Asian J. Pharm. Ana. 2016; 6(1): 23-30. DOI : 10.5958/2231-5675.2016.00004.1
4.    Shivabasappa PM, Mardolkar S, Suryawanshi S. New validated ultra high performance liquid chromatographic method for estimation of Ranolazine hydrochloride. Pharma Journal 2019; 8(7): 382–5.
5.    Patel Vishakha D, Raj Hasumati A, Gheewala Nirav. Pharmacology of Combined Amiodarone Hydrochloride and Ranolazine Therapy in Atrial Fibrillation. Asian J. Res. Pharm. Sci. 2015; 5(4): 234-238.
6.    Lakshmi Sivasubramanian, d K.S. Lakshmi. Absorbance Correction H-Point Standard Addition Method for Simultaneous Spectrophotometric Determination of Ramipril, Hydrochlorothiazide and Telmisartan in Tablets. Asian J. Research Chem. 2015; 8(2): 69-73. DOI:10.5958/0974-4150.2015.00014.0.
7.    DVS Roopa Sirisha Doppa, Sathish Kumar Konidala, Sheik Khanabhi. Development and Validation of UV Spectroscopic Method for the Determination of Ranolazine in Bulk and Formulation. Research J. Pharm. and Tech. 2019; 12(10): 5007-5010. DOI : 10.5958/0974-360X.2019.00868.0
8.    Killedar S, Pawar A, Nadaf S, Nale A, Tamboli U, Pishawikar S. Novel analytical method development for some amide group containing drugs using Bougainvillea spectabilis bract extracts. Asian Pac J Trop Med. 2014; 7(S1): S560–7.
9.    Joshi H. Spectrophotometric Method For The Estimation of Ranolazine. Unique Research Journal of Chemistry 2014; 2(1): 5–7.
10.    Yabré M, Ferey L, Somé IT, Gaudin K. Greening reversed-phase liquid chromatography methods using alternative solvents for pharmaceutical analysis. Molecules. 2018; 23(5).
11.    Reem H. Obaydo, Amir Alhaj Sakur. A Green Analytical Method using Algorithm (PCCA) for Extracting Components’ Contribution from Severely Overlapped Spectral Signals in Pharmaceutical Mixtures. Research J. Pharm. and Tech 2019; 12(9): 4332-4338 DOI : 10.5958/0974-360X.2019.00745.5.
12.    Ola Mahmoud Younes, Fida Am Ali, Zaid Al Assaf. Enantioseparation of Metoprolol Tartrate using HPLC by Adding Methyl beta Cyclodextrin to the mobile Phase (As Chiral Additive). Research J. Pharm. and Tech 2018; 11(9): 3937-3942. DOI : 10.5958/0974-360X.2018.00723.0
13.    K. Hemalatha, G. Madhumitha. Eco-friendly Synthesis of Palladium Nanoparticles, Environmental Toxicity Assessment and its Catalytic Application in Suzuki Miyaura Coupling. Research J. Pharm. and Tech. 2015; 8(12): 1691-1700. DOI : 10.5958/0974-360X.2015.00305.4
14.    González-Ruiz V, León AG, Olives AI, Martín MA, Menéndez JC. Eco-friendly liquid chromatographic separations based on the use of cyclodextrins as mobile phase additives. Green Chem. 2011; 13(1): 115–26.
15.    Bi W, Li S, Row KH. Eco-friendly separation of catechins using cyclodextrins as mobile phase additives in RP-HPLC. Phytochem Anal. 2012; 23(4): 308–14. doi:abs/10.1002/pca.1359
16.    ICH-Q2(R1) 2005. International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use ICH Harmonised Tripartite Guideline Validation of Analytical Procedures: Text and Methodology Q2(R1). Federal Register, Vol. 62, No. 96. 2015.
17.    Szente L, Szemán J. Cyclodextrins in analytical chemistry: Host-guest type molecular recognition. Anal Chem. 2013; 85(17): 8024–30. doi:10.1021/ac400639y
18.    Kammoun, A. K. et al. Development of validated methods for the simultaneous quantification of Finasteride and Tadalafil in newly launched FDA-approved therapeutic combination: greenness assessment using AGP, analytical eco-scale, and GAPI tools. RSC Advances.  13(17): 11817–11825. doi: 10.1039/d3ra01437a.
19.    Noha Ibrahim, Eman S. Elzanfaly, Ahmed E. El Gendy, Said A. Hassan. Development, Optimization, and Validation of a Green Spectrofluorimetric method for the determination of Moxifloxacin using an Experimental design approach. Research Journal of Pharmacy and Technology. 2021; 14(4): 1880-6. DOI : 10.52711/0974-360X.2021.00332.

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