Author(s):
Henny Syapitri, Santhna Letchmi Panduragan, Satheesh Babu Natarajan, Cut Masyitah Thaib, Agnes Purba, Rinawati Sembiring, Ruma Poddar
Email(s):
heny_syahfitri86@yahoo.com
DOI:
10.52711/0974-360X.2024.00216 
Address:
Henny Syapitri1, Santhna Letchmi Panduragan2, Satheesh Babu Natarajan2, Cut Masyitah Thaib1, Agnes Purba1, Rinawati Sembiring1, Ruma Poddar2
1Faculty of Pharmacy and Health Science, Sari Mutiara Indonesia University, Jln. Kapten Muslim No.79 Medan, North Sumatra, Indonesia.
2Lincoln University College, Wisma Lincoln, 12-18, Jalan SS 6/12, 47301 Petaling Jaya, Selangor D. E., Malaysia.
*Corresponding Author
Published In:
Volume - 17,
Issue - 3,
Year - 2024
ABSTRACT:
Black cumin (Nigella sativa) seed oil contains thymoquinone and saponins, can be used to treat inflammation. This study aims to obtain a nanoparticle emulsion gel preparation of black cumin seed oil that has anti-inflammatory activity of wound healing in rabbit experimental animals. The preparation was made with a gel base (F1), a gel formula with black cumin extract (F2), and a black cumin extract gel formula with nanoparticle emulsion (F3). Evaluation of the preparation was carried out for eight weeks, including pH, organoleptic, homogeneity, dispersibility, and stability tests. A particle size analyzer (PSA) test was carried out to assess the size of nanoparticles. The activity test was carried out by observing the wound. The results of the pH test of the three formulations in the normal skin pH interval are 4.5–6.5. The evaluation results of the three formulations showed they were stable. The results of the homogeneity test of the three formulations showed that the preparations were homogeneous during storage. The gel spreadability test is good, with a minimum and maximum range of 5 to 7 cm after being given a load of 150 g and a very comfortable semisolid consistency.
Cite this article:
Henny Syapitri, Santhna Letchmi Panduragan, Satheesh Babu Natarajan, Cut Masyitah Thaib, Agnes Purba, Rinawati Sembiring, Ruma Poddar. Formulation and Wound healing activity of Nano Particle Emulsion Gel containing Nigella sativa seed oil in rabbits. Research Journal of Pharmacy and Technology. 2024; 17(3):1370-5. doi: 10.52711/0974-360X.2024.00216
Cite(Electronic):
Henny Syapitri, Santhna Letchmi Panduragan, Satheesh Babu Natarajan, Cut Masyitah Thaib, Agnes Purba, Rinawati Sembiring, Ruma Poddar. Formulation and Wound healing activity of Nano Particle Emulsion Gel containing Nigella sativa seed oil in rabbits. Research Journal of Pharmacy and Technology. 2024; 17(3):1370-5. doi: 10.52711/0974-360X.2024.00216 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2024-17-3-67
REFERENCE:
1. LeMone PT, Burke KM, Gerene Bauldoff RN, Gubrud P. Medical-surgical nursing: Clinical reasoning in patient care. Pearson; 2014.
2. Brown HL, Clayton A, Stephens P. The role of bacterial extracellular vesicles in chronic wound infections: Current knowledge and future challenges. Wound Repair and Regeneration. 2021; 29(6): 864-80. https://doi.org/10.1111/wrr.12949
3. Ojueromi OO, Oboh G, Ademosun AO. Black Seed (Nigella sativa): A Favourable Alternative Therapy for Inflammatory and Immune System Disorders. Inflammopharmacology. 2022: 1-21. https://doi.org/10.1007/s10787-022-01035-6
4. Rashid M, Ganaie MA, Khan S, Akhtar N, Ahmad M, Shams S, Bilal OS, Bisht D. Comparative antibacterial study of black cumin oil of Saudi and Syrian origin seeds with the commercial product. Journal of Reports in Pharmaceutical Sciences. 2021; 10(1): 148. https://doi.org/10.4103/jrptps.JRPTPS_118_20
5. Santiworakun NY, Dahlan W, Arour Z, Plalamee N, Sirikwanpong S, Ontao N, Marpae M, Suksuwan A. Formulation and Stability Determination of Anti-Acne Cream Containing Black Cumin Seed Oil and Kaolin Clay. InProceedings of The International Halal Science and Technology Conference. 2022; 14(1): 153-160. https://doi.org/10.31098/ihsatec.v14i1.497
6. Zouirech O, Alyousef AA, El Barnossi A, El Moussaoui A, Bourhia M, Salamatullah AM, Ouahmane L, Giesy JP, Aboul-Soud MA, Lyoussi B, Derwich E. Phytochemical analysis and antioxidant, antibacterial, and antifungal effects of essential oil of black caraway (Nigella sativa L.) seeds against drug-resistant clinically pathogenic microorganisms. BioMed Research International. 2022; 2022. https://doi.org/10.1155/2022/5218950
7. Karsono Tanuwijaya J, Fatma D. Formulation of ibuprofen orally disintegrating tablets (ODTs) by Lyophilization method using gelatin and mannitol. Int J PharmTech Res. 2014; 6(3): 996-1002.
8. Mujaddidi N, Nisa S, Al Ayoubi S, Bibi Y, Khan S, Sabir M, Zia M, Ahmad S, Qayyum A. Pharmacological properties of biogenically synthesized silver nanoparticles using endophyte Bacillus cereus extract of Berberis lyceum against oxidative stress and pathogenic multidrug-resistant bacteria. Saudi Journal of Biological Sciences. 2021; 28(11): 6432-40. https://doi.org/10.1016/j.sjbs.2021.07.009
9. Talat M, Zaman M, Khan R, Jamshaid M, Akhtar M, Mirza AZ. Emulgel: An effective drug delivery system. Drug Development and Industrial Pharmacy. 2021; 47(8): 1193-9. https://doi.org/10.1080/03639045.2021.1993889
10. Murray F, Solan M, Douglas A. Effects of algal enrichment and salinity on sediment particle reworking activity and associated nutrient generation mediated by the intertidal polychaete Hediste diversicolor. Journal of Experimental Marine Biology and Ecology. 2017 Oct 1;495:75-82. https://doi.org/10.1016/j.jembe.2017.06.002
11. Barradas TN. de Holanda e Silva KG. Nanoemulsions of essential oils to improve solubility, stability and permeability: a review. Environ. Chem. Lett. 2021; 19: 1153-71. https://doi.org/10.1007/s10311-020-01142-2
12. Cascella M, Bimonte S, Barbieri A, Del Vecchio V, Muzio MR, Vitale A, Benincasa G, Ferriello AB, Azzariti A, Arra C, Cuomo A. Dissecting the Potential Roles of Nigella sativa and Its Constituent Thymoquinone on the Prevention and on the Progression of Alzheimer's Disease. Frontiers in Aging Neuroscience. 2018; 10: 16. https://doi.org/10.3389/fnagi.2018.00016
13. Tiwari P, Jena S, Satpathy S, Sahu PK. Nigella sativa: phytochemistry, pharmacology and its therapeutic potential. Research Journal of Pharmacy and Technology. 2019; 12(7): 3111-6. https://doi.org/10.5958/0974-360X.2019.00526.2
14. Sorg H, Tilkorn DJ, Hager S, Hauser J, Mirastschijski U. Skin wound healing: an update on the current knowledge and concepts. European Surgical Research. 2017; 58(1-2): 81-94. https://doi.org/10.1159/000454919
15. Jockenhöfer F, Gollnick H, Herberger K, Isbary G, Renner R, Stücker M, Valesky E, Wollina U, Weichenthal M, Karrer S, Kuepper B. Aetiology, comorbidities and cofactors of chronic leg ulcers: retrospective evaluation of 1 000 patients from 10 specialised dermatological wound care centers in Germany. International Wound Journal. 2016; 13(5): 821-8. https://doi.org/10.1111/iwj.12387
16. Khader M, Eckl PM. Thymoquinone: an emerging natural drug with a wide range of medical applications. Iranian journal of basic medical sciences. 2014; 17(12): 950.
17. Darakhshan S, Pour AB, Colagar AH, Sisakhtnezhad S. Thymoquinone and its therapeutic potentials. Pharmacological research. 2015; 95:138-58. https://doi.org/10.1016/j.phrs.2015.03.011
18. Milne KE, Penn-Barwell JG. Classification and management of acute wounds and open fractures. Surgery (Oxford). 2020; 38(3): 143-9. https://doi.org/10.1016/j.mpsur.2020.01.010
19. de Moura Estevão LR, Cassini-Vieira P, Leite AG, de Carvalho Bulhões AA, da Silva Barcelos L, Evêncio-Neto J. Morphological evaluation of wound healing events in the excisional wound healing model in rats. Bio-protocol. 2019; 9(13): e3285-. https://doi.org/10.21769/BioProtoc.3285
20. Bura AR. Effect of Wound Healing Potential of Plumeria obtusa (Champa) Spray. Asian Journal of Pharmaceutical Research. 2018; 8(4): 231-5. https://doi.org/10.5958/2231-5691.2018.00039.4
21. Amminbavi D, Lakshmi NP. Assessment of In vitro wound healing potential of Hibiscus leaf extract Emulgel. Asian Journal of Pharmaceutical Research. 2020; 10(2): 67-72. https://doi.org/10.5958/2231-5691.2020.00013.1
22. Rawat S, Gupta A. Development and study of wound healing activity of an ayurvedic formulation. Asian Journal of Research in Pharmaceutical Science. 2011; 1(1): 26-8.
23. Purohit SK, Solanki R, Soni R, Mathur V. Evaluation of wound healing activity of ethanolic extract of Azadirachta Indica leaves in male albino rats. Asian Journal of Pharmacy and Technology. 2013; 3(2): 73-5.
24. Jain S, Jain N, Tiwari A, Balekar N, Jain DK. Simple evaluation of wound healing activity of polyherbal formulation of roots of Ageratum conyzoides Linn. Asian Journal of Research in Chemistry. 2009; 2(2): 135-8.
25. Ghori SS, Gouse M, Panda N, Khaled S, Basheeruddin S, Baig MD, Yaqub M, Mufaqqum M, Nazneen A, Butul AZ. Wound Healing Activity of Poly Herbal formulation. Research Journal of Pharmaceutical Dosage Forms and Technology. 2015; 7(2): 125-8. https://doi.org/10.5958/0975-4377.2015.00018.X
26. Shenoy C, Patil MB, Kumar R. Antibacterial and wound healing activity of the leaves of Annona squamosa Linn.(Annonaceae). Research Journal of Pharmacognosy and phytochemistry. 2009; 1(1): 44-50.
27. Minutti CM, Knipper JA, Allen JE, Zaiss DM. Tissue-specific contribution of macrophages to wound healing. InSeminars in Cell and Developmental Biology. 2017; 61: 3-11. https://doi.org/10.1016/j.semcdb.2016.08.006
28. Mh Busra F, Rajab NF, Tabata Y, Saim AB, BH Idrus R, Chowdhury SR. Rapid treatment of full‐thickness skin loss using ovine tendon collagen type I scaffold with skin cells. Journal of Tissue Engineering and Regenerative Medicine. 2019; 13(5): 874-91. https://doi.org/10.1002/term.2842
29. Takeo M, Lee W, Ito M. Wound healing and skin regeneration. Cold Spring Harbor Perspectives in Medicine. 2015; 5(1): a023267.https://doi.org/10.1101/cshperspect.a023267
30. Parnell LK, Volk SW. The evolution of animal models in wound healing research: 1993–2017. Advances in Wound Care. 2019; 8(12): 692-702. https://doi.org/10.1089/wound.2019.1098
31. Etulain J. Platelets in wound healing and regenerative medicine. Platelets. 2018; 29(6): 556-68. https://doi.org/10.1080/09537104.2018.1430357
32. Tracy LE, Minasian RA, Caterson EJ. Extracellular matrix and dermal fibroblast function in the healing wound. Advances in Wound Care. 2016; 5(3): 119-36. https://doi.org/10.1089/wound.2014.0561
33. Dunnill C, Patton T, Brennan J, Barrett J, Dryden M, Cooke J, Leaper D, Georgopoulos NT. Reactive oxygen species (ROS) and wound healing: the functional role of ROS and emerging ROS‐modulating technologies for augmentation of the healing process. International Wound Journal. 2017;14(1):89-96. https://doi.org/10.1111/iwj.12557
34. Espinosa-Diez C, Miguel V, Mennerich D, Kietzmann T, Sánchez-Pérez P, Cadenas S, Lamas S. Antioxidant responses and cellular adjustments to oxidative stress. Redox Biology. 2015 ;6: 183-97. https://doi.org/10.1016/j.redox.2015.07.008
35. Prescott C, Bottle SE. Biological relevance of free radicals and nitroxides. Cell Biochemistry and Biophysics. 2017; 75(2): 227-40. https://doi.org/10.1007/s12013-016-0759-0
36. Fatima F, Aleemuddin M, Ahmed MM, Anwer M, Aldawsari MF, Soliman GA, Mahdi WA, Jafar M, Hamad AM, Alshehri S. Design and Evaluation of Solid Lipid Nanoparticles Loaded Topical Gels: Repurpose of Fluoxetine in Diabetic Wound Healing. Gels. 2023; 9(1): 21. https://doi.org/10.3390/gels9010021