Author(s):
Asriullah Jabbar, Halik, Fadhliyah Malik, Muhammad Ilyas Y, Jamal Qadar, Hasyrul Hamzah, Sylvia Utami Tunjung Pratiwi
Email(s):
sylvia_pratiwi@ugm.ac.id
DOI:
10.52711/0974-360X.2024.00257 
Address:
Asriullah Jabbar1,2,8, Halik2, Fadhliyah Malik2, Muhammad Ilyas Y2,3, Jamal Qadar4, Hasyrul Hamzah5,8, Sylvia Utami Tunjung Pratiwi6,7,8*
1Post Doctoral Program, Faculty of Pharmacy, Universitas Gadjah Mada, Jl. Farmako Sekip Utara, Yogyakarta, Indonesia, 55281.
2Faculty of Pharmacy, Universitas Halu Oleo, Jl. H.E.A Mokodompit, Kendari, Indonesia, 93232.
3Politeknik Bina Husada Kendari, 93117, Indonesia.
4Puangrimaggalatung University, Sengkang, 90911, Indonesia.
5Faculty of Pharmacy, Universitas Muhammadiyah Kalimantan Timur, Samarinda, Kalimantan Timur 75124, Indonesia.
6Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, 55281 Indonesia.
7Medical Plants and Natural Products Research Center Faculty of Pharmacy, Universitas Gadjah Mada Jl. Farmako Sekip Utara, Yogyakarta, Indonesia, 55281.
8Indonesia Biofilm Research Collaboration Center (IBRCC), Jl. Farmako Sekip Utara, Yogyakarta, Indonesia, 55281.
*Corresponding Author
Published In:
Volume - 17,
Issue - 4,
Year - 2024
ABSTRACT:
Etlingera rubroloba A.D Poulsen (E. rubroloba) plants from the genus Etlingera, empirically used as traditional medicine by several ethnicities in Southeast Sulawesi, namely to increase endurance and relieve joint pain. The purpose of this study was to determine the antihyperuricemia activity on uric acid levels and cyclooxygenase-2 (COX-2) levels in vivo in mice. This study used six groups of test animals consisting of normal, negative (Na-CMC) 0.5%, positive control (allopurinol) and dose groups of ethanol extract of E. rubroloba fruit 100, 200 and 300 mg/g bw. Modeling of hyperuricemia test animals is done by inducing potassium oxonate. Measurement of uric acid levels by lateral chromatography method and COX-2 levels by ELISA method. The results showed that ethanol extract of E. rubroloba fruit can reduce uric acid and COX-2 levels (p<0.05). The results of the correlation statistical test showed a positive correlation between uric acid levels and COX-2 levels, where the decrease in uric acid levels was directly proportional to the decrease in COX-2 levels. This research can be a reference in the development of traditional medicine, especially anti-hyperuricemia.
Cite this article:
Asriullah Jabbar, Halik, Fadhliyah Malik, Muhammad Ilyas Y, Jamal Qadar, Hasyrul Hamzah, Sylvia Utami Tunjung Pratiwi. Antihyperurisemia Activity of Etanol Extract of Etlingera rubroloba A.D Poulsen Fruits on Uric Acid and Cyclooxygenase-2 Levels In Vivo. Research Journal of Pharmacy and Technology.2024; 17(4):1627-1. doi: 10.52711/0974-360X.2024.00257
Cite(Electronic):
Asriullah Jabbar, Halik, Fadhliyah Malik, Muhammad Ilyas Y, Jamal Qadar, Hasyrul Hamzah, Sylvia Utami Tunjung Pratiwi. Antihyperurisemia Activity of Etanol Extract of Etlingera rubroloba A.D Poulsen Fruits on Uric Acid and Cyclooxygenase-2 Levels In Vivo. Research Journal of Pharmacy and Technology.2024; 17(4):1627-1. doi: 10.52711/0974-360X.2024.00257 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2024-17-4-31
REFERENCES:
1. Abujbara M, Al Hourani HM, Al-Raoush RI, Khader YS, Ajlouni K. Prevalence of Hyperuricemia and Associated Factors Among Type 2 Diabetic Patients in Jordan. Int J Gen Med. 2022; 15: 6611-6619. doi:10.2147/IJGM.S376857
2. N B, M G, C R. Correlation of Salivary Uric Acid and Cardiac Autonomic Modulation in Metabolic Syndrome Population. Research Journal of Pharmacy and Technology. 2023; 16(3): 1347-1350. doi:10.52711/0974-360X.2023.00221
3. K M, Krishnan RS, Rahini P. A Review on Gouty Arthritis. Research Journal of Pharmacy and Technology. 2019; 12(11): 5583-5588. doi:10.5958/0974-360X.2019.00967.3
4. Nisa U, Astana PRW, Kuncoro H. The effect of Antihypertensive Herb formula of Indonesian traditional medicines against serum uric acid levels in mild Hypertensive patients. Research Journal of Pharmacy and Technology. 2021; 14(1): 254-258. doi:10.5958/0974-360X.2021.00045.7
5. Kumaresan GD, M D. Efficacy of Cox-2 inhibitors in the Clinical Management of TMJ Arthritis: A Review. Research Journal of Pharmacy and Technology. 2017; 10(12): 4439-4441. doi:10.5958/0974-360X.2017.00817.4
6. S B, G S, Ys R. Future of Cancer Therapy-COX-2 Inhibitors: A Review. Research Journal of Pharmacy and Technology. 2009; 2(4): 617-620.
7. Oğuz N, Kırça M, Çetin A, Yeşilkaya A. Effect of uric acid on inflammatory COX-2 and ROS pathways in vascular smooth muscle cells. J Recept Signal Transduct Res. 2017; 37(5): 500-505. doi:10.1080/10799893.2017.1360350
8. Jabbar A, Hamzah H, Nandini E, et al. The Effectiveness of Begonia Multangula Blume Leaf Ethanol Extract as Polymicrobial Antibiofilm on Catheters. Egyptian Journal of Chemistry. 2022; 65(13). doi:10.21608/ejchem.2022.118622.5341
9. Tiwari VK, Mishra BB. Opportunity, Challenge and Scope of Natural Products in Medicinal Chemistry. Research Signpost; 2011.
10. Abdel-Hady H, Morsi EA, El-Wakil EA. In-vitro Antimicrobial Potentialities of Phylunthus emblica Leaf Extract Against Some Human Pathogens. Egypt J Chem. 2022; 7: 7.
11. Jabbar A, Wahyuono S, Puspitasari I, Sahidin I. Free radical scavenging activity of methanol extract and compounds isolated from stems of Etlingera rubroloba A.D Poulsen. International Journal Of Pharmaceutical Research. 2021; 13(1): 1099-1105. doi:org/10.31838/ijpr/2021.13.01.478
12. Ilyas M Y, Diantini A, Halimah E, et al. Potential Immunomodulator Fraction Fruit Of Etlingera rubroloba A.D Poulsen Against Macrophage Phagocytosis And Interleukin-12 Levels In BCG-Stimulated Balb/C Mice. International Journal Of Pharmaceutical Research. 2021; 13(1): 3262-3269. doi:org/10.31838/ijpr/2021.13.01.478
13. Y MI, Diantini A, Ghozali M, Sahidin I, Fristiohady A. Immunomodulatory Potency Etlingera rubroloba A.D. Poulsen Fruit Ethanol extract against Macrophage Phagocytic Activity and CD4 Levels in Wistar Male Rats. Research Journal of Pharmacy and Technology. 2022; 15(9): 4067-4072. doi:10.52711/0974-360X.2022.00682
14. Jabbar A, Wahyuono S, Puspitasari I, Sahidin I. Xanthine Oxidase Inhibitory Activity and DPPH radical scavenging Assay of isolated compound from Etlingera rubroloba (Blume) A.D Poulsen stem. International Journal Of Pharmaceutical Research. 2021; 13(1): 1994-2002. doi:https://doi.org/10.3188/ijpr/2021.13.01.316
15. Jabbar A, Sahidin I, Monstavevi S, Malaka M, Malik F, Ilyas MY. Antioxidant and Anti-Inflammatory Activity of Ethanol Extract Stem of Etlingera rubroloba A.D. Poulsen. Pakistan Journal of Biological Sciences. 2022; 25(10): 885-891. doi:10.3923/pjbs.2022.885.891
16. Jabbar A, Sahidin I, Triaqilah KR, Mubarak M, Yusuf MI. Characteristic Test and Antihyperuricemia Activity of Ethanol Extract Stem of Etlingera rubroloba A.D Poulsen In vitro. Medula. 2022; 9(2): 69-77. doi:10.46496/medula.v9i2.25290
17. Fristiohady A, Wahyuni W, Ilyas Y. M, et al. Hepatoprotective activity of Etlingera elatior (Jack) R.M. Smith Extract against CCl4 -induced Hepatic Toxicity in Male Wistar Rats. Research Journal of Pharmacy and Technology. 2020; 13: 2020. doi:10.5958/0974-360X.2020.00807.0
18. Chan E, Lim Y, Omar M. Antioxidant and antibacterial activity of leaves of Etlingera species (Zingiberaceae) in Peninsular Malaysia. Food Chemistry. 2007; 104: 1586-1593. doi:10.1016/j.foodchem.2007.03.023
19. Ilyas Y. M, Sahidin I, Jabbar A, et al. Effect of Immunomodulating Extract and Some Isolates from Etlingera rubroloba A.D. Poulsen Fruits on Diabetic Patients with Tuberculosis. Molecules. 2023; 28(5): 2401. doi:10.3390/molecules28052401
20. Solichah AI, Anwar K, Rohman A, Fakhrudin N. Phytochemical Profile and Antioxidant Activity of Some Artocarpus Genus Plants in Indonesia. Journal of Food and Pharmaceutical Sciences. Published online July 26, 2021:443-460. doi:10.22146/jfps.2026
21. Sonia R, Yusnelti Y, Fitrianingsih F. The Effectivity of Ethanol Extracts of Durio zibethinus (Linn.) Leaves as Antihyperuricemia. Jurnal Kefarmasian Indonesia. Published online August 24, 2020: 130-139. doi:10.22435/jki.v10i2.2148
22. Sunarni T, Leviana F, Fidrianny I, Immculata M, Wirasutisna KR. Antihyperuricemic And Xanthine Oxidase Inhibitory Activities Of Fractions From Ethanolic Leaves Extract of Stelechocarpus Burahol. Asian J Pharm Clin Res. 2016; 9(6): 255. doi:10.22159/ajpcr.2016.v9i6.14314
23. Mohammed OJ, Saeed AM, Mohammed IS. RP–HPLC Developed Method for Uric Acid Estimation in Human Serum. Research Journal of Pharmacy and Technology. 2019; 12(10): 4703-4708. doi:10.5958/0974-360X.2019.00810.2
24. Mohamed AL, Elmotasem H, Salama AAA. Colchicine mesoporous silica nanoparticles/hydrogel composite loaded cotton patches as a new encapsulator system for transdermal osteoarthritis management. Int J Biol Macromol. 2020; 164: 1149-1163. doi:10.1016/j.ijbiomac.2020.07.133
25. Weletnsae T, Hintsa K, Tadege T, et al. Phytochemical analysis and In vitro screening of putative dual COX-2 / 5-LOX inhibitors from Cucumis ficifolius root as an Anti-inflammatory agent. Research Journal of Pharmacy and Technology. 2019; 12(11): 5240-5246. doi:10.5958/0974-360X.2019.00906.5
26. Jabbar A, Wahyuni W, Leorita M, Yusuf MI, Salsabila H, Sahidin I. Antihyperuricemia Activity of Wualae Fruit (Etlingera elatior Jack R. M. Smith) Ethanol Extract In Vivo. JFSP. Published online. 2022: 104-111. doi:10.31603/pharmacy.v1i1.6083
27. Wahyuni W, Yusuf MI, Malik F, Lubis AF, Indalifiany A, Sahidin I. Immunomodulatory Effects of Ethanol Extract of Melophlus sarasinorum Sponge Against Phagocytosis Activity of Macrophage Cells on Balb/C Male Mice. 1. 2019; 5(2): 147-157. doi:10.22487/j24428744.2019.v5.i2.13611
28. Wells AF, MacDonald PA, Chefo S, Jackson RL. African American patients with gout: efficacy and safety of febuxostat vs allopurinol. BMC Musculoskeletal Disorders. 2012; 13(1): 15. doi:10.1186/1471-2474-13-15
29. Siregar GPH, Fadli F. Examination of Blood Uric Acid Levels in the Elderly with the Stick Method at Tanjung Rejo Community Center, Percut Seituan District. Jurnal Online Keperawatan Indonesia. 2018; 1(2): 29-38.
30. Li Y, Yao J, Han C, et al. Quercetin, Inflammation and Immunity. Nutrients. 2016; 8(3): 167. doi:10.3390/nu8030167
31. Widyaningsih W, Edityaningrum CA, Ningsih RS, Hasanah K. Effects of Ethanol Extract of Green Algae (Ulva lactuca L) on Histological Description, COX-1 Expression and COX-2 in Ethanol-Induced Rat’s Gastric. Research Journal of Pharmacy and Technology. 2023; 16(2): 727-732. doi:10.52711/0974-360X.2023.00124
32. Widyaningsih TD, Sari RA, Rukmi WD, Dahniar SA. The Effect of Moringa Leaf (Moringa oleifera Lam.), Pandan Wangi (Pandanus amaryllifolius Roxb.) and Red Ginger (Zingiber officinale Rosc.Var) Extract Supplement Intervention on Uric Acid, Kidney, Liver Function and Perceptions of Hyperuricemia patients on health benefit. Research Journal of Pharmacy and Technology. 2022; 15(10): 4477-4484. doi:10.52711/0974-360X.2022.00751
33. Kırça M, Oğuz N, Çetin A, Uzuner F, Yeşilkaya A. Uric acid stimulates proliferative pathways in vascular smooth muscle cells through the activation of p38 MAPK, p44/42 MAPK and PDGFRβ. J Recept Signal Transduct Res. 2017; 37(2): 167-173. doi:10.1080/10799893.2016.1203941