Author(s): Karlina Amir Tahir, Erwin Hafid, Muh. Fitrah, Ahmad Lalo, Nurul Fadilah, Syamsuri Syakri, Syatirah Jalaluddin, Katsuyoshi Matsunami

Email(s): karlina.amir@uin-alauddin.ac.id

DOI: 10.52711/0974-360X.2024.00309   

Address: Karlina Amir Tahir1, Erwin Hafid2, Muh. Fitrah1, Ahmad Lalo3, Nurul Fadilah1, Syamsuri Syakri1, Syatirah Jalaluddin1, Katsuyoshi Matsunami4
1Faculty of Medicine and Health Science, UIN Alauddin Makassar, Makassar, Indonesia.
2Master of Islamic Education Management, UIN Alauddin Makassar, Makassar, Indonesia.
3National Agency of Drug and Food Control, Makassar, Indonesia.
4Department of Pharmacognosy, Faculty of Biomedical and Health Science at Hiroshima University, Japan.
*Corresponding Author

Published In:   Volume - 17,      Issue - 5,     Year - 2024


ABSTRACT:
Cancer can usually develop due to exposure to sunlight. UV radiation from sunlight is known to damage DNA and is bad for the skin. Skin P stem cell carcinogenesis is caused by UV-A rays that penetrate deep into the dermis layer. UV-B damages cell DNA by being absorbed by proteins in the epidermis. Chromolaena odorata was extracted using methanol solvent, then partitioned into 5 solutions in the form of n-Hexane, Ethyl Acetate, Acetonitrate, n-Buthanol, and Ethanol. The five extracts obtained were tested with Human Epidermal Keratinocyte cells using the bioassay method. Results obtained from the microplate reader after incubation. Each extract was divided into three concentrations, it is 100, 50, 20(µg/mL). Then in the positive control (Etoposide), it was divided into four concentrations, 100, 50, 20, 10(µg/mL). After being analyzed with the results of the microplate reader, the IC50 of Chromolaena odorata was 48% in the ethyl acetate extract with a concentration of 100µg/mL. HaCaT cell proliferation was determined at indicated intervals using the MTT colorimetric assay. This assay was based on the ability of live cell succinate dehydrogenase to reduce the yellow salt MTT (3-(4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide)) (Sigma-Aldrich, St. Louis, MO, USA) to insoluble purple-blue formazan precipitate. Experiments were carried out on 96-well plates containing a final volume of 100µl of medium/well.


Cite this article:
Karlina Amir Tahir, Erwin Hafid, Muh. Fitrah, Ahmad Lalo, Nurul Fadilah, Syamsuri Syakri, Syatirah Jalaluddin, Katsuyoshi Matsunami. Inhibition of Skin Cancer using Human Epidermal Keratinocytes (HaCaT) Cells from Siam Weeds (Chromolaena odorata L.). Research Journal of Pharmacy and Technology. 2024; 17(5):1951-5. doi: 10.52711/0974-360X.2024.00309

Cite(Electronic):
Karlina Amir Tahir, Erwin Hafid, Muh. Fitrah, Ahmad Lalo, Nurul Fadilah, Syamsuri Syakri, Syatirah Jalaluddin, Katsuyoshi Matsunami. Inhibition of Skin Cancer using Human Epidermal Keratinocytes (HaCaT) Cells from Siam Weeds (Chromolaena odorata L.). Research Journal of Pharmacy and Technology. 2024; 17(5):1951-5. doi: 10.52711/0974-360X.2024.00309   Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2024-17-5-5


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