Author(s): Karlina Amir Tahir, Erwin Hafid, Muh. Fitrah, Ahmad Lalo, Nurul Fadilah, Syamsuri Syakri, Syatirah Jalaluddin, Katsuyoshi Matsunami

Email(s): karlina.amir@uin-alauddin.ac.id

DOI: 10.52711/0974-360X.2024.00309   

Address: Karlina Amir Tahir1, Erwin Hafid2, Muh. Fitrah1, Ahmad Lalo3, Nurul Fadilah1, Syamsuri Syakri1, Syatirah Jalaluddin1, Katsuyoshi Matsunami4
1Faculty of Medicine and Health Science, UIN Alauddin Makassar, Makassar, Indonesia.
2Master of Islamic Education Management, UIN Alauddin Makassar, Makassar, Indonesia.
3National Agency of Drug and Food Control, Makassar, Indonesia.
4Department of Pharmacognosy, Faculty of Biomedical and Health Science at Hiroshima University, Japan.
*Corresponding Author

Published In:   Volume - 17,      Issue - 5,     Year - 2024


ABSTRACT:
Cancer can usually develop due to exposure to sunlight. UV radiation from sunlight is known to damage DNA and is bad for the skin. Skin P stem cell carcinogenesis is caused by UV-A rays that penetrate deep into the dermis layer. UV-B damages cell DNA by being absorbed by proteins in the epidermis. Chromolaena odorata was extracted using methanol solvent, then partitioned into 5 solutions in the form of n-Hexane, Ethyl Acetate, Acetonitrate, n-Buthanol, and Ethanol. The five extracts obtained were tested with Human Epidermal Keratinocyte cells using the bioassay method. Results obtained from the microplate reader after incubation. Each extract was divided into three concentrations, it is 100, 50, 20(µg/mL). Then in the positive control (Etoposide), it was divided into four concentrations, 100, 50, 20, 10(µg/mL). After being analyzed with the results of the microplate reader, the IC50 of Chromolaena odorata was 48% in the ethyl acetate extract with a concentration of 100µg/mL. HaCaT cell proliferation was determined at indicated intervals using the MTT colorimetric assay. This assay was based on the ability of live cell succinate dehydrogenase to reduce the yellow salt MTT (3-(4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide)) (Sigma-Aldrich, St. Louis, MO, USA) to insoluble purple-blue formazan precipitate. Experiments were carried out on 96-well plates containing a final volume of 100µl of medium/well.


Cite this article:
Karlina Amir Tahir, Erwin Hafid, Muh. Fitrah, Ahmad Lalo, Nurul Fadilah, Syamsuri Syakri, Syatirah Jalaluddin, Katsuyoshi Matsunami. Inhibition of Skin Cancer using Human Epidermal Keratinocytes (HaCaT) Cells from Siam Weeds (Chromolaena odorata L.). Research Journal of Pharmacy and Technology. 2024; 17(5):1951-5. doi: 10.52711/0974-360X.2024.00309

Cite(Electronic):
Karlina Amir Tahir, Erwin Hafid, Muh. Fitrah, Ahmad Lalo, Nurul Fadilah, Syamsuri Syakri, Syatirah Jalaluddin, Katsuyoshi Matsunami. Inhibition of Skin Cancer using Human Epidermal Keratinocytes (HaCaT) Cells from Siam Weeds (Chromolaena odorata L.). Research Journal of Pharmacy and Technology. 2024; 17(5):1951-5. doi: 10.52711/0974-360X.2024.00309   Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2024-17-5-5


REFERENCES:
1.    WHO (2020) Skin cancers. https://www.who.int/uv/faq/skincancer/en/ index1.htm
2.    Apalla Z, Nashan D, Weller RB, Castellsagué X. Skin cancer: epidemiology, disease burden, pathophysiology, diagnosis, and therapeutic approaches. Dermatol Ther (Heidelb). 2017; 7(Suppl 1):5-19. doi: 10.1007/s13555-016-0165-y
3.    Akila Victor, Muhammad Rukunuddin Ghalib. Detection of skin cancer cells – a review. Research J.Pharm and Tech; 2017; 10(11):4093-4098. doi: 10.5958/0974-360X.2017.00742.9
4.    Scott G. Demonstration of melanosome transfer by a shedding microvesicle mechanism. J Invest Dermatol. 2012; 132(4):1073-4. doi: 10.1038/jid.2012.20
5.    Nasti TH, Timares L. MC1R, eumelanin and pheomelanin: their role in determining the susceptibility to skin cancer. Photochem Photobiol. 2015; 91(1):188-200. doi: 10.1111/php.12335
6.    Keerthana Karunakaran, Jansi Rani Malaiyappan, Rajini Raja Muniyan. Protein-protein interaction of mutated agouti signaling protein (asip) to melanocortin receptor 1 (mc1r) in melanoma skin cancer: an insilico study. Research J. Pharm. and Tech 2018; 11(9): 3913-3917. doi: 10.5958/0974-360X.2018.00718.7
7.    Min Jeong Kim 1, Seo Yeon Shin 1, Nu Ri Song 1, Sunoh Kim 2, Sang Ouk Sun 1 and Kyung Mok Park 1. Bioassay-guided characterization, antioxidant, anti-melanogenic and anti-photoaging activities of pueraria thunbergiana l. leaf extracts in human epidermal keratinocytes (hacat) cells. MDPI 10. 2022; 2156. https://doi.org/10.3390/ pr10102156
8.    Singh M, Suman S, Shukla Y. New enlightenment of skin cancer chemoprevention through phytochemicals: in vitro and in vivo studies and the underlying mechanisms. Biomed Res Int. 2014; 2014:243452. doi: 10.1155/2014/243452
9.    Qadir MI. Skin cancer: etiology and management. Pak J Pharm Sci. 2016; 29(3):999-1003
10.    Aba, P.E., Joshua, P.E., Ezeonuogu, F.C., Ezeja, M.I., Omoja, V.U., Umeakuana, P.U. Possible anti-diarrhoeal potential of ethanol leaf extract of Chromolaena odorata in castor oil-induced rats. J. Complement. Integr. Med. 12. 2015; 301–306. https://doi. org/10.1515/jcim-2014-0033
11.    Femi Olawalea,b, Kolawole Olofinsanc, Opeyemi Iwaloyed. Biological activities of chromolaena odorata: A mechanistic review. Elsevier. 2021; https://doi.org/10.1016/j.sajb.2021.09.001
12.    Hung, T.M., Cuong, T.D., Dang, N.H., Zhu, S., Long, P.Q., Komatsu, K., Min, B.S. Flavonoid glycosides from chromolaena odorata leaves and their in vitro cytotoxic activity. Chem. Pharm. Bull. 59. 2011; 129–131. https://doi.org/10.1248/cpb.59.129
13.    Ajay, A., Kumar, R., Badhusha, S., Abhishek, K., Gowda, S.K., Ramesh, B. Pharmacological importance of chromolaena odorata: a review. Int. J. Pharm. Drug Anal Vol 9. 2021; 8–11. Doi:10.47957/ijpda.v9i1.452
14.    Vijayaraghavan, K., Rajkumar, J., Seyed, M.A (2018). Phytochemical screening, free radical scavenging, and antimicrobial potential of chromolaena odorata leaf extracts against pathogenic bacterium in wound infectionsa multispectrum perspective. Biocatal. Agric. Biotechnol. 15, 103–112. 20. https://doi.org/10.1016/j.bcab.2018.05.014
15.    Nath, L.R., Gorantla, J.N., Joseph, S.M., Antony, J., Thankachan, S., Menon, D.B., Sankar, S., Lankalapalli, R.S., Anto, R.J. Kaempferide, the most active among the four flavonoids isolated and characterized from chromolaena odorata, induces apoptosis in cervical cancer cells while being pharmacologically safe. RSC Adv. 5. 2015; 100912– 100922. https://doi.org/10.1039/C5RA19199H
16.    Mkhobongo B, Chandran R, Abrahamse H. In vitro photodynamic treatment modality for a375 melanoma cell line using a sulphonated aluminum phthalocyanine chloride-photosensitizer-gold nanoparticle conjugate. Pharmaceutics. 2022; 16;14(11):2474. doi: 10.3390/pharmaceutics14112474
17.    Irma Colombo, Erico Sangiovanni, Roberta Maggio, et all. Hacat cells as a reliable in vitro differentiation model to dissect the inflammatory/repair response of human keratinocytes. Mediator of Inflammation. 2017; https://doi.org/10.1155?2017/7435621
18.    Pastar I, Stojadinovic O, Yin NC, Ramirez H, Nusbaum AG, Sawaya A, Patel SB, Khalid L, Isseroff RR, Tomic-Canic M. Epithelialization in wound healing: a comprehensive review. Adv Wound Care (New Rochelle). 2014; 3(7):445-464. doi: 10.1089/wound.2013.0473
19.    Micallef L, Belaubre F, Pinon A, Jayat-Vignoles C, Delage C, Charveron M, Simon A. Effects of extracellular calcium on the growth-differentiation switch in immortalized keratinocyte hacat cells compared with normal human keratinocytes. Exp Dermatol. 2009; 18(2):143-51. doi: 10.1111/j.1600-0625.2008.00775.x
20.    Pedrosa TDN, Catarino CM, Pennacchi PC, Assis SR, Gimenes F, Consolaro MEL, Barros SBM, Maria-Engler SS. A new reconstructed human epidermis for in vitro skin irritation testing. Toxicol In Vitro. 2017; 42:31-37. doi: 10.1016/j.tiv.2017.03.010
21.    Yajarla, V.N.G., Nimmanapalli, R.P., Parikapandla, S., Gupta, G., Karnati, R. Anti-inflammatory, and anti-proliferative properties of Chromolaena odorata leaf extracts in normal and skin-cancer cell lines. J. Herbs. Spices Med. Plants 20. 2014; 359–371. doi:10.1080/10496475.2013.876698
22.    Wilson VG. Growth and differentiation of hacat keratinocytes. Methods Mol Biol. 2014; 1195:33-41. doi: 10.1007/7651_2013_42
23.    Tammineni Sreelatha, M. V. Subramanyam, M. N. Giri Prasad. A survey work on early detection methods of melanoma skin cancer. Research J. Pharm. and Tech. 2019; 12(5):2589-2596. doi: 10.5958/0974-360X.2019.00435.9
24.    Micali G, Lacarrubba F, Nasca MR, Ferraro S, Schwartz RA. Topical pharmacotherapy for skin cancer: part ii. Clinical applications. Journal of the American Academy of Dermatology. 2014; 70(6):979.e1-12; quiz 9912. doi: 10.1016/j.jaad.2013.12.037.
25.    Navdeep Singh, Shivi Sondhi, Sanyam Sharma, Dheeraj Singh, Vishal Koundal, Kamya Goyal, Shammy Jindal. Treatment of skin cancer by topical drug delivery of nanoparticles: a review. Research Journal of Pharmacy and Technology. 2021; 14(10):5589-8. doi: 10.52711/0974-360X.2021.00973
26.    Zainab, Nurlailah, Muhammad Ikhwan Rizki. Identification of active compound and antibacterial activity against gram-positive and gram-negative bacteria of chromolaena odorata leaf extract. Research Journal of Pharmacy and Technology. 2022; 15(10):4720-6. doi: 10.52711/0974-360X.2022.00793
27.    Nishita Gogia, Anandhi D, Kanaga G, Revathi K. In vitro evaluation of anti-mitotic activity of green synthesized silver nanoparticles from ethanolic extract of chromolaena odorata, caesalpinia coriaria (bark and leaves) using allium cepa roots. Research Journal of Pharmacy and Technology. 2021; 14(8):4107-0. doi: 10.52711/0974-360X.2021.00711
28.    Eze FN, Jayeoye TJ. Chromolaena odorata (Siam weed): A natural reservoir of bioactive compounds with potent anti-fibrillogenic, antioxidative, and cytocompatible properties. Biomed Pharmacother. 2021;141:111811. doi: 10.1016/j.biopha.2021.111811.
29.    Madhulika Pradhan, S Sribhuwaneswari, D Karthikeyan, Sunita Minz, Pavani Sure, Atul N Chandu, Umesh Mishra, K Kamalakannan, A Saravanankumar, T Sivakumar. In-vitro cytoprotection activity of foeniculum vulgare and helicteres isora in cultured human blood lymphocytes and antitumour activity against b16f10 melanoma cell line. Research J. Pharm. and Tech. 2018; 1(4):450-452
30.    Radha Krishna Kollipara, Vyshnavi Tallapaneni, Bharat Kumar Reddy Sanapalli, Vinoth Kumar G, Veera Venkata Satyanarayana Reddy Karri. Curcumin loaded ethosomal vesicular drug delivery system for the treatment of melanoma skin cancer. Research Journal of Pharmacy and Technology. 2019; 12(4):1783-1792. doi: 10.5958/0974-360X.2019.00298.1
31.    Perumal S, Langeswaran K. Diosmin anti-tumour efficacious against hepatocellular carcinoma. Research J. Pharm. and Tech. 2020; 13(4):1707-1714. doi: 10.5958/0974-360X.2020.00308.X
32.    Ghadge Ojaswi, Nadar Divya, Parmar Digna. Melanoma and its drug targets. Research J. Pharm. and Tech. 2016; 9(5): 562-570. doi: 10.5958/0974-360X.2016.00107.4

Recomonded Articles:

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

1.3
2021CiteScore
 
56th percentile
Powered by  Scopus


SCImago Journal & Country Rank

Journal Policies & Information


Recent Articles




Tags


Not Available