Author(s):
Siti Nurbaya, Numlil Khaira Rusdi, Diyah Kristianti, Eldafira, Nurhuda Sahar, Rosmalena, Conny Riana Tjampakasari, Berna Elya, Rozana Othman, Maifitrianti, Anna Wirdiani Fathiah
Email(s):
numlilkhaira@uhamka.ac.id
DOI:
10.52711/0974-360X.2025.00875 
Address:
Siti Nurbaya1, Numlil Khaira Rusdi2*, Diyah Kristianti1, Eldafira3, Nurhuda Sahar3, Rosmalena4, Conny Riana Tjampakasari1, Berna Elya5, Rozana Othman6, Maifitrianti7, Anna Wirdiani Fathiah8
1Department of Pathology Clinic, Faculty of Medicine, University of Indonesia.
2Magister of Pharmacy Education Program, School of Postgraduate, Universitas Muhammadiyah Prof. Dr. Hamka, Jakarta, Indonesia.
3Department Biology, Faculty of Medicine, University of Indonesia, Jakarta.
4Department of Chemistry, Faculty of Medicine, University of Indonesia, Jakarta.
4Department of Microbiology Faculty of Medicine, University of Indonesia, Jakarta.
5Faculty of Pharmacy, University of Indonesia, Jakarta.
6Faculty of Chemistry, University of Malaya.
7Faculty of Pharmacy, Universitas Muhammadiyah Prof. Dr. Hamka, Jakarta, Indonesia.
8Master Program in Biomedical Science, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia.
*Corresponding Author
Published In:
Volume - 18,
Issue - 12,
Year - 2025
ABSTRACT:
Overweight (Obesity) and administration of ovarian stimulation regimen have a negative impact on endometrial receptivity during implantation. It is known that the receptive condition of the endometrium during the embryo implantation reception period is characterized by the expression of various receptivity markers, one of which is E-Cadherin. The purpose of this study was to analyze and compare the level of E-Cadherin expression in the endometrium of normal weight female rats with a group of stimulated normal weight rats, a group of overweight rats and a group of stimulated overweight rats. overweight, obesity and normal weight in the stimulated and unstimulated groups. Methods, 3-month-old female Wistar rats with a normal body weight of around 200grams and overweight rats (given a high-calorie fat diet) for 2-3months), an average body weight of 275grams. Normal weight rats were stimulated once with recombinant FSH (Gonal F) intraperitoneally at a dose of 15–20IU and overweight rats were injected with a dose of 20-25 IU FSH in the diestrus phase. 48 hours after injection (estrus phase), the rats were mated with male rats (Vaginal plug). Endometrium sampling was performed serially on days 3, 4, and 5 after mating. E-Cadherin expression was examined using immunohistochemical staining (IHK). Using the IHK score calculation formula = (% positive high cell contribution x 4) + (% positive cell contribution x 3) + (% positive low cell contribution x 2) + (% negative cell contribution 1), Results, E-Cadherin expression in the endometrium of rats in the normal weight group did not show a significant difference in expression levels compared to the stimulated normal weight rat group (P = 0.07). When compared with the group of overweight mice that were not stimulated, there was a significant increase in expression levels (P = 0.02). Furthermore, with the expression of E-Cadherin in the group of overweight mice that were stimulated, there was a significant increase (P = 0.01). Conclusion, E-Cadherin expression increased significantly in overweight female mice, both stimulated and unstimulated, compared with normal weight mice.
Cite this article:
Siti Nurbaya, Numlil Khaira Rusdi, Diyah Kristianti, Eldafira, Nurhuda Sahar, Rosmalena, Conny Riana Tjampakasari, Berna Elya, Rozana Othman, Maifitrianti, Anna Wirdiani Fathiah. Changes in E-Cadherin Expression Levels between Obese and Normal Rat after Gonadotropin Hormone Stimulation. Research Journal Pharmacy and Technology. 2025;18(12):6053-8. doi: 10.52711/0974-360X.2025.00875
Cite(Electronic):
Siti Nurbaya, Numlil Khaira Rusdi, Diyah Kristianti, Eldafira, Nurhuda Sahar, Rosmalena, Conny Riana Tjampakasari, Berna Elya, Rozana Othman, Maifitrianti, Anna Wirdiani Fathiah. Changes in E-Cadherin Expression Levels between Obese and Normal Rat after Gonadotropin Hormone Stimulation. Research Journal Pharmacy and Technology. 2025;18(12):6053-8. doi: 10.52711/0974-360X.2025.00875 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2025-18-12-65
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