Author(s):
Madhuri Sunil Mohite Patil, Hemant Kumar Singh Yadav, Abhay Raizaday
Email(s):
msmohite.2008@yahoo.com , hemant.dops@igu.ac.in , abhayraizadaypharma@gmail.com
DOI:
10.52711/0974-360X.2025.00469 
Address:
Madhuri Sunil Mohite Patil1, Hemant Kumar Singh Yadav2, Abhay Raizaday3
1Gourishankar Institute of Pharmaceutical Education and Research, Limb, Satara, Maharashtra.
2Department of Pharmaceutical Sciences, Indira Gandhi University, Meerpur, Rewari.
3Gyan Vihar School of Pharmacy, Suresh Gyan Vihar University, Jaipur, Rajasthan, India.
*Corresponding Author
Published In:
Volume - 18,
Issue - 7,
Year - 2025
ABSTRACT:
Endometrial carcinoma is the most common gynecological cancer. The number of new cases is increasing worldwide day by day. Increases in malignancy have encourages researches to develop new treatment modalities for this cancer. Severe side effects and limitations in the therapeutic efficiency of conventional cancer treatments have led to the widespread use of complementary and alternative medicines. Among the various treatment modalities, traditional medicines with demonstrable anti-cancer activity are the preferred choice. Borassus flabellifer Linn. is a traditional herb. Each and every part of plant shows different types of pharmacological actions, flowers are used in the treatment of the diabetes, very useful for bilious affections, enlarged liver, spleen, act as cooling and used in uterus tumors. However, the anti-proliferative activity in endometrial cancer cells has yet to be not elucidated. This study investigated the anticancer effects of various extract of, Borassus flabellifer Linn. flowers on Ishikawa cells. The flowers of Borassus flabellifer Linn. were collected from Pune district. Authenticated at Botanical Survey of Pune. The extraction was performed using the Soxhlet device with petroleum ether, ethyl acetate, chloroform and hydro-alcoholic solvent. Flowers were also extracted by cold maceration. Preliminary phytochemical investigation revels extract contains carbohydrates, proteins, steroids, triterpenoids, glycosides, flavonoids, tannins and phenolic compounds. The in vitro anticancer study was performed on Ishikawa cell line with sulforhodamine B assay in TATA Memorial, ACTREC Mumbai as per National Cancer Institute guidelines. The present experiment was repeated thrice and then average percentage of control growth was calculated and compared. In in- vitro experiment the average value of GI50-80 µg/mL in case of (EA) Ethyl acetate extract of Borassus flabellifer Linn. Flowers was 61.9 while in case of pure compound, the average value of GI50-80 µg/mL was -35.1. Our results demonstrate that Borassus flabellifer Linn. Flowers extracts inhibits the growth of cancer cells which suggests the potential applicability of Borassus flabellifer Linn.Flowersas an anticancer agent.
Cite this article:
Madhuri Sunil Mohite Patil, Hemant Kumar Singh Yadav, Abhay Raizaday. The effect of Borassus flabellifer Linn. Flower extract on human Endometrial Cancer Ishikawa cell lines In - vitro. Research Journal of Pharmacy and Technology. 2025;18(7):3258-1. doi: 10.52711/0974-360X.2025.00469
Cite(Electronic):
Madhuri Sunil Mohite Patil, Hemant Kumar Singh Yadav, Abhay Raizaday. The effect of Borassus flabellifer Linn. Flower extract on human Endometrial Cancer Ishikawa cell lines In - vitro. Research Journal of Pharmacy and Technology. 2025;18(7):3258-1. doi: 10.52711/0974-360X.2025.00469 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2025-18-7-49
REFERENCES:
1. Mihai-Vlad Vălu, Ovidiu Toma Endometrial cancer. A review and evaluation of risk factors. Article in Analele științifice ale Universității "Al. I. Cuza" din Iași. Secțiunea IIIc, Științe economice (1976) October 2017 Research Gate 129-136. https://www.researchgate.net/publication/320592508.
2. Jens C. Hahne, Susanne R. Meyer, Johannes Dietl and Arnd Honig.The effect of Cordyceps extract and a mixture of Ganoderma lucidum/Agaricus Blazi Murill extract on human endometrial cancer cell lines in vitro. International Journal of Oncology. doi: 10.3892/ijo.2014.2414. 45: 373-382, 2014.
3. https://www.healthgrades.com/right-care/cancer/types-and-stages-of-uterine-cancer May 19, 2020.
4. Johnson IS, Armstrong JG, Gorman M, Burnett JP Jr. The vinca alkaloids: a new class of oncolytic agents. Cancer Res. 1963; 23: 1390–1427. doi: 10.1016/b978-1-4831-9929-0.50010-3.
5. Moon JY, Mosaddik A, Kim H, et al. The chloroform fraction of Guava leaf extract inhibits human gastric cancer cell proliferation via induction of apoptosis. In Food Chem. 2011; 125: 369–375. doi:10. 1089/jmf.2011.1701
6. Mrs. Asia chatterjee, Dr. Satyesh Chandra prakashit. The Treastis on Indian Medicinal Plants CSIR New Delhi 1992: 14.
7. K.M. Nadbarni, Indian Material Madica Vol-1st A.K. Nadbarni, Popular Prakashan-Pvt Ltd; 1976
8. Kapoor LD, Handbook of Ayurvedic Medicinal Plants 1st edition Washington: CRC Press; 2005,82
9. The Wealth of India. A Dictionary of Indian Raw materials and Industrial products. Publications and Information directorate CSIR New Delhi; 2B 187-198
10. Arya Vaidya Sala Indian Medicinal Plants Chennai; Orient Longaman Ltd. 1996; 293.
11. Dr JC. Kurian Plant That Heal Oriental Watchman Publishing House, Pune, and India.
12. Khandelwal K. R. Practical Pharmacognosy. New Delhi: Vallabh Prakashan; 1994:146-48.
13. Sandeep Charak1 Monika Sharma Sharad M. Porte. In Vitro Evaluation of Cytotoxicity and Anticancer Activity of Herbal Extract V2S2 (Coded Drug) on Human Cancer Cell Lines. Asian Journal of Oncology. 2022-07-04. doi: 10.1055/s-0041-1735335.
14. Vanicha Vichai and Kanyawim Kirtikara. Sulforhodamine B colorimetric assay for cytotoxicity screening Nature Protocols 1: 1112–1116, 2006. doi: 10.1038/nprot.2006. 179.
15. Jyoti Kode, Jeshma Kovvuri, Burri Nagaraju, Shailesh Jadhav, Madan Barkume, Subrata Sen, Nirmal Kumar Kasinathan, Pradip Chaudhari, Bhabani Shankar Mohanty, Jitendra Gour, Dilep Kumar Sigalapalli, C. Ganesh Kumar, Trupti Pradhan, Manisha Banerjee and Ahmed Kamal. Synthesis, biological evaluation and molecular docking analysis of phenstatin based indole linked chalcones as anticancer agents and tubulin polymerization inhibitors. In Bioorganic Chemistry. 2020; 105: 104447. DOI: 10.1016/j.bioorg.2020.104447.
16. Faisal Kholiya, Shruti Chatterjee, Gopal Bhojani, Subrata Sen, Madan Barkume, Nirmal Kumar Kasinathan, Jyoti Kode, Ramavatar Meena. Seaweed polysachharide derived bioaldehyde nanocomposite: Potential application in anticancer therapeutics. Carbohydrate Polymers. Online published 2020; 20(4): 240 doi:10.1016/j.carbpol.2020.116282. 2020
17. Skehan, P.; Storeng, R.; Scudiero, D.; Monks, A.; McMahon, J.; Vistica, D.; Warren, J.T.; Bokesch, H.; Kenney, S.; Boyd, M.R. New colorimetric cytotoxicity assay for anticancer-drug screening. J Natl Cancer Inst. 1990; 82: 1107-1112. doi: 10.1093/jnci/82.13.1107
18. Prasanth DSNBK, Srinivasa Rao A, Rajendra Prasad Y. Anticancer activity of isolated constituents from Aralia racemosa L and Argyreia pilosa wight and arn by sulphorhodamine (SRB) assay on ishikawa and SCC–29B cell Lines. MOJ Bioequivalence and Bioavailability. 2018; 5(1): 1‒9. doi: 10.15406/mojbb.2018.05. 00074
19. Venkateswarlu G, Raja sundararajan. Anticancer Activity of Methanol extract of Limnophila repens and Argyeia cymosa by Using SRB Assay. Research Journal of Pharmacy and Technology. 2023; 16(3): 1459-1462 doi: 10.52711/0974-360X.2023. 00240.