Author(s):
Chandana Choudhury Barua, SM Abdul Aziz Barbhuiya, Syamprasad Np, Ajay Kakati, Lipika Buragohain, Suhena Shirin Barbhuiya
Email(s):
chanacin@gmail.com
DOI:
10.52711/0974-360X.2025.00476 
Address:
Chandana Choudhury Barua1*, SM Abdul Aziz Barbhuiya2, Syamprasad Np3, Ajay Kakati1, Lipika Buragohain1, Suhena Shirin Barbhuiya4
1Department of Pharmacology and Toxicology, College of Veterinary Science, Assam Agricultural University, Guwahati, Assam - 781022, India.
2University of Science and Technology, Meghalaya, Techno City, Kiling Road, Baridua, 9th Mile, Ri-Bhoi, Meghalaya – 793101.
3Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research (NIPER) Guwahati, Assam, India.
4NETES Institute of Pharmaceutical Science, Mirza, Kamrup - 781125, Assam, India.
*Corresponding Author
Published In:
Volume - 18,
Issue - 7,
Year - 2025
ABSTRACT:
In this exploratory research MEEP was screened against various cancer cell lines (HeLa, MDA MB 231, DU 145, A 549, and HepG2) and phaseoloidin against HepG2 cell line. The MTT assay was employed to evaluate the cancer cells' viability. Detection of intracellular ROS by using DCF-DA, measurement of mitochondrial super oxide generation (MitosoxTM Red), mitochondrial membrane potential using JC-1 staining and analyzed by flow cytometer, and molecular features were performed by western blotting. Phaseoloidin showed a promising best result against human hepatic carcinoma cells (HepG2 cells). HepG2 cells were exposed to various concentrations (1.23, 3.70, 11.11, 33.33, and 100 µM) of phaseoloidin, where it was found to trigger the apoptosis dose-dependently on HepG2 cells. This included characteristic changes in nuclear morphology, the breakdown of mitochondrial membrane potential (??m), up-regulation of pro-apoptotic BAX, and down-regulation of anti-apoptotic Bcl-2, which initiates the transformation of caspase-3 to cleaved caspase-3, thus actuating PARP promoting apoptosis.The data showed that Phaseoloidin induces cell death through up-regulation of cellular ROS production. This implies that Phaseoloidin could be a novel anticancer molecule from the natural source in treating hepatic cancer
Cite this article:
Chandana Choudhury Barua, SM Abdul Aziz Barbhuiya, Syamprasad Np, Ajay Kakati, Lipika Buragohain, Suhena Shirin Barbhuiya. Anti-cancer activity of Homogentisic acid 2-O-I-D-glucoside (Phaseoloidin) and Exploration of its underlying Molecular mechanism. Research Journal of Pharmacy and Technology. 2025;18(7):3295-1. doi: 10.52711/0974-360X.2025.00476
Cite(Electronic):
Chandana Choudhury Barua, SM Abdul Aziz Barbhuiya, Syamprasad Np, Ajay Kakati, Lipika Buragohain, Suhena Shirin Barbhuiya. Anti-cancer activity of Homogentisic acid 2-O-I-D-glucoside (Phaseoloidin) and Exploration of its underlying Molecular mechanism. Research Journal of Pharmacy and Technology. 2025;18(7):3295-1. doi: 10.52711/0974-360X.2025.00476 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2025-18-7-56
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