Author(s):
M. Thenmozhi, Vikram E N T, M. Gokul, G. Sathiya Balan
Email(s):
sathiyagbharathi@gmail.com
DOI:
10.52711/0974-360X.2025.00630 
Address:
M. Thenmozhi1, Vikram E N T1, M. Gokul2, G. Sathiya Balan3*
1Department of Pharmacology, Captain Srinivasa Murthy Central Ayurveda Research Institute, CCRAS, M/o AYUSH, Govt. of India, Chennai 600106.
2Department of Chemistry, Captain Srinivasa Murthy Central Ayurveda Research Institute, CCRAS, M/o AYUSH, Govt. of India Chennai 600106.
3Assistant Professor, Department of Pharmacognosy, Madurai Medical College, Madurai - 625020.
*Corresponding Author
Published In:
Volume - 18,
Issue - 9,
Year - 2025
ABSTRACT:
Aim and Objective: Eucalyptus globulus L. belongs to the family Myrtaceae, a natural insecticide widely used as anti -microbial, anti-septic, analgesic, etc. The objective was to formulate gel formulation(Carbopol-940) from essential oil of Eucalyptus globulus and to evaluate its efficacy. Materials and Methods: Eucalyptus oil was fractionated from the leaves of Eucalyptus globulus and characterized by GC-MS Instrumentation technique to detect the presence of active phytoconstituents. Then it is made into a gel formulation containing 12.5% v/v of active ingredient and then evaluated for stability study. The formulated gel was evaluated for its mosquito repellent potential using the laboratory method, arm in cage and the time of protection (% mosquito repellence) was calculated based on comparison with control arm. For determining the larvicidal activity, different concentrations of formulated E. globulus oil gel 0.1%, 0.2%, 0.3%, 0.4% v/v was prepared from the stock solution (5:25 Cineole: Acetone) and were tested against 4th instar larvae of Aedes and Culex species. Mortality rate was recorded at 3, 6, 12, 24 and 48hrs as per the standard protocol. Results: Characterization data from GC-MS revealed the presence of active phytoconstituent 1,8 Cineole. The % of mosquito repellence at consecutive time points was found to be stable without much deviation. The larvicidal activity against species of larva- Culex and Aedes showed mortality rate at 0.3% v/v concentration with effective LC50 values of 2625.418ppm for Aedes and 2448.979ppm for Culex species with 95% CI. (MicroProbit 3.0 software). Conclusion: The formulated gel from Eucalyptus globulus was found to have potential application as mosquito repellent, larvicidal action and used as an alternative to synthetic products.
Cite this article:
M. Thenmozhi, Vikram E N T, M. Gokul, G. Sathiya Balan. Mosquito Repellent and Larvicidal activity of a Gel Formulation from the Essential Oil of Eucalyptus globulus. Research Journal of Pharmacy and Technology. 2025;18(9):4396-0. doi: 10.52711/0974-360X.2025.00630
Cite(Electronic):
M. Thenmozhi, Vikram E N T, M. Gokul, G. Sathiya Balan. Mosquito Repellent and Larvicidal activity of a Gel Formulation from the Essential Oil of Eucalyptus globulus. Research Journal of Pharmacy and Technology. 2025;18(9):4396-0. doi: 10.52711/0974-360X.2025.00630 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2025-18-9-50
REFERENCES:
1. Muller-Heupt LK, Vierengel N, Groß J, Opatz T, Deschner J, von Loewenich FD. Antimicrobial Activity of Eucalyptus globulus, Azadirachta indica, Glycyrrhiza glabra, Rheum palmatum Extracts and Rhein against Porphyromonas gingivalis. Antibiotics (Basel). 2022; 31; 11(2): 186. doi: 10.3390/antibiotics11020186. PMID: 35203789; PMCID: PMC8868162.
2. Khan A, Bisanzio D, Mutuku F, Ndenga B, Grossi-Soyster EN, Jembe Z, Maina PW, Chebii PK, Ronga CO, Okuta V, LaBeaud AD. Spatiotemporal overlapping of dengue, chikungunya, and malaria infections in children in Kenya. BMC Infect Dis. 2023; 29; 23(1): 183. doi: 10.1186/s12879-023-08157-4. PMID: 36991340; PMCID: PMC10053720.
3. Tizifa TA, Kabaghe AN, McCann RS, van den Berg H, Van Vugt M, Phiri KS. Prevention Efforts for Malaria. Curr Trop Med Rep. 2018; 5(1): 41-50. doi: 10.1007/s40475-018-0133-y. Epub 2018 Feb 8. PMID: 29629252; PMCID: PMC5879044.
4. Haleem ZM, Yadav S, Cushion ML, Tanner RJ, Carek PJ, Mainous AG. Exposure to N,N-Diethyl-Meta-Toluamide Insect Repellent and Human Health Markers: Population Based Estimates from the National Health and Nutrition Examination Survey. Am J Trop Med Hyg. 2020; 103(2): 812-814. doi: 10.4269/ajtmh.20-0226. Epub 2020 May 21. PMID: 32458781; PMCID: PMC7410448.
5. Cmikova N, Galovicova L, Schwarzová M, Vukic MD, Vukovic NL, Kowalczewski PŁ, Bakay L, Kluz MI, Puchalski C, Kacaniová M. Chemical Composition and Biological Activities of Eucalyptus globulus Essential Oil. Plants (Basel). 2023; 12(5): 1076. doi: 10.3390/plants12051076. PMID: 36903935; PMCID: PMC10004840.
6. Rehman A, Iqbal M, Khan BA, Khan MK, Huwaimel B, Alshehri S, Alamri AH, Alzhrani RM, Bukhary DM, Safhi AY, Hosny KM. Fabrication, In Vitro, and In Vivo Assessment of Eucalyptol-Loaded Nanoemulgel as a Novel Paradigm for Wound Healing. Pharmaceutics. 2022; 14(9): 1971. doi: 10.3390/pharmaceutics14091971. PMID: 36145720; PMCID: PMC9500607.
7. Barnard DR. Repellency of essential oils to mosquitoes (Diptera: Culicidae). J. Med. Entomol. 1999; 36: 625–629.
8. Amer A, Mehlhorn H. Repellency effect of forty-one essential oils against Aedes, Anopheles and Culex mosquitoes. Parasitol. Res. 2006a; 99: 478–490.
9. Amer A, Mehlhorn H. Larvicidal effects of various essential oils against Aedes, Anopheles, and Culex larvae (Diptera, Culicidae). Parasitol. Res. 2006b; 99: 466–472. Google Scholar
10. Aulena DN, Purba AV, Djamil R. Formulation and Evaluation of Gel Contains the Combination of Ethanol Extract Basil leaves (Ocimum sanctum L.) and Soursop Leaves (Annona muricata L.) as a Mosquito Repellent. Int J Pharm Pharm Res. 2016; 7(2): 10-18.
11. Barnard DR, Xue RD. Laboratory evaluation of mosquito repellents against Aedes albopictus, Culex nigripalpus, and Ochlerotatus triseriatus (Diptera: Culicidae). J. Med. Entomol. 2004; 41: 726–730.
12. Muturi EJ, Selling GW, Doll KM, Hay WT, Ramirez JL. Leptospermum scoparium essential oil is a promising source of mosquito larvicide and its toxicity is enhanced by a biobased emulsifier. PLoS One. 2020; 15(2): e0229076. doi: 10.1371/journal.pone.0229076. PMID: 32078653; PMCID: PMC7032722.
13. Salehi B, Upadhyay S, Erdogan Orhan I, Kumar Jugran A, L D Jayaweera S, A Dias D, Sharopov F, Taheri Y, Martins N, Baghalpour N, Cho WC, Sharifi-Rad J. Therapeutic Potential of α- and β-Pinene: A Miracle Gift of Nature. Biomolecules. 2019; 9(11): 738. doi: 10.3390/biom9110738. PMID: 31739596; PMCID: PMC6920849.