Author(s):
Tamilvanan K, P.V Murali Krishna, Vanita Somasekhar, Rajeshkumar R
Email(s):
pallamurali86@gmail.com
DOI:
10.52711/0974-360X.2026.00011 
Address:
Tamilvanan K1, P.V Murali Krishna2*, Vanita Somasekhar3, Rajeshkumar R4
1Department of Pharmaceutical Analysis, KLE College of Pharmacy, Bengaluru - 560 010, Karnataka, India.
2Associate Professor, Department of Pharmaceutical Analysis, KLE College of Pharmacy, Bengaluru - 560 010, Karnataka, India.
3Professor, Department of Pharmaceutical Analysis, KLE College of Pharmacy, Bengaluru - 560 010, Karnataka, India.
4Assistant Manager, QC Department, Natural Remedies Pvt Ltd, Bengaluru - 560 100, Karnataka, India.
*Corresponding Author
Published In:
Volume - 19,
Issue - 1,
Year - 2026
ABSTRACT:
The study aimed to identify and quantify andrographolides in various marketed samples of A. Paniculata and detect pesticide residues. It aimed to improve understanding of the composition, purit, and safety.The TLC technique used an aluminium plate with pre-coated silica gel (E. Merck – Silica Gel 60F254). Samples were spotted using a Linomat IV syringe TLC applicator (CAMAG) and a solvent system consisting of acetone, benzene, and chloroform (20:20:10 ratio). With a LiChrospher 100 RP-18e (5µm) column, a quaternary pump, UV detector, auto injector, column oven, and LC-Solution software, the HPLC system comprised a SHIMADZU LC-2030C Liquid Chromatograph. The Agilent Gas Chromatograph with Electron Capture and Nitrogen Phosphorus Detector 7890A-ECD/NPD (EZ Chrome) and associated equipment, along with a Zebron ZB-1 column (30 m in length, 0.25 mm in diameter, and 0.50 m film thickness), made up the GC-ECD system.The TLC results showed well-developed andrographolide spots in all samples, with sample C having the highest percentage. HPLC quantification confirmed this, with sample C exhibiting the highest total andrographolide content. GC-ECD analysis detected pesticide residues in A. paniculata samples. This comprehensive chromatographic analysis enhances our understanding of A. paniculata composition and purity, ensuring potency and safety in herbal products. These findings provide valuable insights for quality control in A. paniculata-based formulations.Our research emphasises the safety and therapeutic potential of Andrographis paniculata, underscoring its importance in contemporary medicine. By using cutting-edge methods for thorough study, we were able to verify its effectiveness against several diseases.
Cite this article:
Tamilvanan K, P.V Murali Krishna, Vanita Somasekhar, Rajeshkumar R. A Comprehensive Analysis of Andrographis paniculata Herbal Products Conducted to Enhance Potency and Ensure Safety Through the Application of Pertinent Chromatographic Techniques.. Research Journal of Pharmacy and Technology. 2026;19(1):69-5. doi: 10.52711/0974-360X.2026.00011
Cite(Electronic):
Tamilvanan K, P.V Murali Krishna, Vanita Somasekhar, Rajeshkumar R. A Comprehensive Analysis of Andrographis paniculata Herbal Products Conducted to Enhance Potency and Ensure Safety Through the Application of Pertinent Chromatographic Techniques.. Research Journal of Pharmacy and Technology. 2026;19(1):69-5. doi: 10.52711/0974-360X.2026.00011 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2026-19-1-11
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