Author(s):
V. Bhawani, A. Perumal
Email(s):
bhawanikmc@gmail.com , perumalanat1sas@gmail.com
DOI:
10.52711/0974-360X.2026.00022 
Address:
V. Bhawani1, A. Perumal2
1Full Time Research Scholar, Department of Anatomy, Srinivasan Medical College and Hospital, Dhanalakshmi Srinivasan University, Samayapuram, Tiruchirappalli - 621112.
2Professor, Department of Anatomy, Srinivasan Medical College and Hospital, Dhanalakshmi Srinivasan University, Samayapuram, Tiruchirappalli - 621112.
*Corresponding Author
Published In:
Volume - 19,
Issue - 1,
Year - 2026
ABSTRACT:
Background: Amaranthus caudatus herb is popularly known as Inca wheat. Amaranthus caudatus leaves are used by the indigenous tribes for treating various pulmonary ailments and hepatorenal conditions. Besides it is also used as an antidiarrheal and anti-ulcer agent but there is lack in literature about the compounds responsible for these actions. Previous researches have shown the preliminary phytocomponents present in cold methanolic leaf extract of Amaranthus caudatus by GC-MS analysis. This study was conducted to reveal the difference in bioactive materials and the antioxidant properties present in hot methanolic leaf extract of Amaranthus caudatus along with its uses. Aim: To gain knowledge about the specific bioactive materials present in the hot methanolic leaf extract of Amaranthus caudatus by various analysis and its application in various ailments. Material and Methods: The seeds of Amaranthus caudatus were collected from NBPGR - Shimla Center. GC-2010 and QP-2020 SHIMADZU Japan corporation analyzers were used to find the list of phytochemicals with the help of National Institute of Standards and Technology 20M Library Search Programmes. DPPH, total phenol and total flavonoid present in current plant extract were detected with standard methods. The statistical analysis was done by linear correlation with content in graph pad prism software and statistical significance was finalized by one way ANOVA in SPPS software. Results: The presence of 74 different phytochemicals, major compounds which shows a retention time above 20 include octadecanoic acid (20.258), benzene acetic acid (20.342), 9H-pyridol (3,4-b) indole, cis-sinapyl alcohol (20.741), 6-methylheptanoicacid (21.010), chondrillasterol (25.610), hexadecenoic acid (26.220), 1-heptanol (28.24), 13-docosenamide Z(28.984). The mean value of total flavonoid present was detected as 0.44mg/ml in terms of quercetin after triplication per gram of dried plant extract. The mean value of total phenol content was detected as 0.58 mg/ml in terms of gallic acid after triplication from dried plant extract. The total antioxidant percentage inhibition detected in terms of ascorbic acid by DPPH assay was 48.93mg/ml. Conclusion: The methanolic leaf extract of Amaranthus caudatus has shown better antioxidant properties due to the presence of Phenol, Flavonoid and various bioactive compounds. The selection of compounds from current plant extracts depends on the target application. The compounds of interest can be isolated and used in various in vivo models.
Cite this article:
V. Bhawani, A. Perumal. Phytochemical Assessment and In vitro Antioxidant Screening in Methanolic Leaf Concentrate of Amaranthus caudatus. Research Journal of Pharmacy and Technology. 2026;19(1):141-6. doi: 10.52711/0974-360X.2026.00022
Cite(Electronic):
V. Bhawani, A. Perumal. Phytochemical Assessment and In vitro Antioxidant Screening in Methanolic Leaf Concentrate of Amaranthus caudatus. Research Journal of Pharmacy and Technology. 2026;19(1):141-6. doi: 10.52711/0974-360X.2026.00022 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2026-19-1-22
REFERENCES:
1. Manish Deygun, et al. Recent Techniques for Extraction of Natural Products. Research J. Pharm and Tech. 2010; 3(3): 644-649. https://rjptonline.org/AbstractView.aspx?PID=2010-3-3-23.
2. Kinghorn AD et al. The Relevance of Higher Plants in Lead Compound discovery programs. Journal of Natural Products. 2011; 74(6): 1539–55. PMID:21650152: PMCID: PMC3158731. https://doi.org/10.1021/np200391c.
3. Raiger HL, Jajoriya. Grain amaranth. Naturally gluten-free superfood grain. Indian Farming. 2023; 73 (3): 24-27. https://epub.icar.org.in/index.php/IndFarm/article/view/131746
4. Alicia Martinez-Lopez M, et al. Nutraceutical value of kiwicha (Amaranthus caudatus L.). Journal of Functional Foods. 2020; 65(103735): 1756-4646. https://doi.org/10.1016/j.jff.2019.103735
5. Ramdwar MN, Chadee ST, Stoute VA. Estimating the potential consumption level of amaranth for food security initiatives in Trinidad. Cogent Food Agric. 2017; 3 (1): 1-14. https://doi.org/10.1080/23311932.2017.1321475
6. Küpper A, Manmathan HK, et al. Population genetic structure in glyphosate-resistant and susceptible palmer amaranth (Amaranthus palmeri) populations using genotyping by sequencing (GBS). Front. Plant Sci. 2018; 9(29): 1-14. doi:10.3389/fpls.2018.00029.
7. Waysehon Assen Abate, et al. The effects of inclusion of amaranthus (Amaranthus caudatus) grain in layers diet on productive performance and egg quality parameters. Livestock Research for Rural Development. 2023; 35(79). 366. http://www.irrd.org/Irrd35/9/3579wasi.html
8. Komal Ch-andral, Preetham Kumar, Deepa Arya et al. Nutraceuticals role in management of urolithiasis. YMER Journal. 2024; 23(03): 340-354. Doi:10.37896/YMER23.03/21.
9. Jimoh MO, Afolayan AJ, Lewu FB. Toxicity and antimicrobial activities of Amaranthus caudatus L. (Amaranthaceae) harvested from formulated soils at different growth stages. J Evid Based Complementary Altern. Med. 2020; 25: 1–11. PMCID: PMC7705779: PMID:33241708. DOI: 10.1177/2515690X209971578.
10. Aswal JS, Bisht BS, Dobhal R, Uniyal DP. Historical Journey with Amaranth. Asian Agrihist.2016, 20: 201–210. https://www.i-scholar.in/index.php/aah/article/view/162723#tp4
11. Hait, Milan. Research Trends in Medical Plant Sciences. Extraction Techniques of Herbal Drugs. Chapter-2.2019; 5(17): 17-33. DOI: 10.22271/ed.book.415.
12. Cowan MM. Plant products as antimicrobial agents. Clin. Microbiol. Rev.1999; 12: 564–582. PMID: PMC8825: PMID: 1051903. DOI: 10.1128/CMR.12.4.564.
13. Borges A, et al. Comparison of Techniques and Solvents on the Antimicrobial and Antioxidant Potential of Extracts from Acacia dealbata and Olea europaea. Antibiotics (Basel). 2020; 9(2): 48. PMID: 32012935; PMCID: PMC7168226. doi: 10.3390/antibiotics9020048.
14. Madhuri M. Deshmukh, et al. Biochemical Screening, Antibacterial and GC-MS Analysis of Ethanolic Extract of Hemidesmus indicus (L) R.Br. root. Research Journal of Pharmacognosy and Phytochemistry. 2019; 11(2): 73-80. doi:10.5958/0975-4385.2019.00014.1.
15. Chanchal Kumari, Meenatchi P. GC-MS analysis of Phyto-constituents and antimicrobial activity of hexane extract of Lanatana camara Linn. Research Journal of Pharmacognosy and Phytochemistry. 2017; 9(2): 115-120. http://dx.doi.org/10.5958/0975-4385.2017.00021.8
16. Madhuri M, et al. Biochemical Screening, Antibacterial and GC-MS Analysis of Ethanolic Extract of Hemidesmus indicus (L) R.Br. root. Research Journal of Pharmacognosy and Phytochemistry. 2019; 11(2): 73-80. doi:10.5958/0975-4385.2019.00014.1.
17. John Refaat, et al. GC-MS studies of Crinum asiaticum L. Leaves and Flowers.Research Journal of Pharmacognosy and Phytochemistry. 2011; 3(5): 232-235. http://rjpponline.org/AbstractView.aspx?PID=2011-3-6-10
18. Ramasubramaniaraja R. Pharmacognostical Phytochemical Including GC-MS Investigation of Ethanolic Leaf Extracts of Abutilon indicum (Linn). Asian Journal of Pharmaceutical Analaysis. 2011; 1(4): 88-92. https://www.researchgate.net/publication/27688664
19. Chanchal Kumari, Deepalakshmi J. Qualitative and GC-MS Analysis of Phytoconstituents of Parthenium hysterophorus Linn. Research Journal of Pharmacognosy and Phytochemistry. 2017; 9(2): 105-110. http://dx.doi.org/10.5958/0975-4385.2017.00019.X
20. Kumar A, et al. In Vitro α-Amylase Inhibition and Antioxidant Activities of Methanolic Extract of Amaranthus Caudatus Linn. Oman Med J. 2011; 26(3): 166-70. PMID: 22043408; PMCID: PMC3191696. doi: 10.5001/omj.2011.40.
21. Koushik Jana, et al. Das. GC-MS Analysis of Phytocomponents of Methanolic Bark Extract of Sterculia foetida. Research Journal of Pharmacy and Technology. 2023; 16(12): 5624-0. doi: 10.52711/0974-360X.2023.00909
22. T. Thilagavathi, G. Kathiravan. Phytochemical Analysis and Antimicrobial Activity of Ethanolic Leaf Extract of Ficus racemosa Linn. Research J. Pharm. and Tech. 2017; 10(2): 537-540. DOI: 10.5958/0974-360X.2017.00107.X
23. Koushik Jana, et al. GC-MS Analysis of Phytocomponents of Methanolic Bark Extract of Sterculia foetida. Research Journal of Pharmacy and Technology. 2023; 16(12): 5624-0. doi: 10.52711/0974-360X.2023.00909
24. Rina Wijayanti, et al. Isolation and Identification of Phytoconstituents from Methanol Extract Parijoto (Medinilla speciosa). Research Journal of Pharmacy and Technology. 2022; 15(10): 4395-4.doi: 10.52711/0974-360X.2022.00737.
25. Girija K, et al. Anti–diabetic and anti–cholesterolemic activity of methanol extracts of three species of Amaranthus. Asian Pacific Journal of Tropical Biomedicine. 2011; 1(2): 133-138. https://doi.org/10.1016/S2221-1691(11)60011-7. 1.
26. Rajabudeen E, et al. GC-MS Analysis of the Methanol Extract of Tephrosia villosa (L.) Pers. Asian J. Research Chem. 2012; 5(11): 1331-1334.
27. Sonia Sharma, et al. Antimicrobial activity of the Soxhlet extraction of Plumbago zeylanica leaf extracts In-vitro conditions. Research J. Pharm. and Tech. 2012; 5(10): 1297-1300.
28. Kanchan Nikam, et al. An Overview of techniques for extracting bioactive components from natural sources. Research Journal of Pharmacy and Technology. 2024; 17(4): 1874-1880. doi:10.52711/0974-360X.2024.00297.
29. Farha Fatma and Dr. Arun Kumar. GCMS Analysis of Phytochemical Constituents and Screening For Anti-Anaemic Activity of the Methanolic extract of Amaranthus caudatus Leaves.World Journal of Pharmaceutical Science and Research. 2024; 3(3): 88-94. DOI: https://doi.org/10.5281/zenodo.11795634
30. Mahmudul Hasan Md, et al. GC-MS Analysis of Bio-active Compounds in Ethanol Extract of Putranjiva roxburghii Wall. Fruit Peel. Pharmacognosy Journal. 2019; 11(1): 146-149. DOI:10.5530/pj.2019.1.24.
31. Bhalodia, Nayan et.al. Evaluation of in vitro Antioxidant activity of Flowers of Cassia fistula Linn. International Journal of Pharm Tech Research. 2011; 3(1): 58-599.
32. Alam, Numer, Sharma, Khaga. Estimation of Phenolic Content, Flavonoid Content, Antioxidant, and Alpha-Amylase Inhibitory Activity of Some Selected Plants from Siraha District Nepal. Asian J Pharm Clin Res. 2020; 13(4); 18-23. doi: 10.22159/ajpcr. 2020.v13i4.36734.
33. Ansar Mehmood, et al. In vitro total phenolics, total flavonoids, antioxidant and antibacterial activities of selected medicinal plants using different solvent systems. BMC Chemistry. 2022; 16(1): 64. DOI: https://doi.org/10.1186/s13065-022-00858-2
34. Devyana Dyah Wulandari, et al. Total Phenolic, Flavonoid content, and Antioxidant properties of Fermented Honey-Garlic in Hyperlipidemia Rats. Research Journal of Pharmacy and Technology. 2023; 16(9): 4085-2. doi: 10.52711/0974-360X.2023.00669
35. Krishna Kondragunta V, Karuppuraj V, Perumal K. Antioxidant activity and Folic acid content in indigenous isolates of Ganoderma lucidum. Asian J. Pharm. 2016; 6(4): 213-215.
36. Ghanshyam B, et al. Free radical Scavenging and Antioxidant Activity of Punica granatum Linn. Asian J. Res. Pharm. Sci. 2014; 4(2): 51-54.
37. Umavandhana R, Jayanthi S. Analysis of Phytochemical compounds and DPPH radical scavenging activity of Dictyotadichotoma and Halimedamacroloba. Research J. Pharm. and Tech. 2018; 11(8): 3440-3444. doi: 10.5958/0974-360X.2018.00635.2.