Author(s):
Lia Kusmita, Marshanda Amelia, I Kadek Bagiana, Aninditia Sabdaningsih
Email(s):
lia_kusmita@yahoo.com
DOI:
10.52711/0974-360X.2026.00024 
Address:
Lia Kusmita1*, Marshanda Amelia1, I Kadek Bagiana1, Aninditia Sabdaningsih2
1STIFAR Yayasan Pharmasi Semarang, Letjend Sarwo Edhie Wibowo KM 1 50193 Plamongansari Semarang, Central Java, Indonesia.
2Postgraduate Program of Aquatic Resources Management, Department of Aquatic Resources, Faculty of Fisheries and Marine Sciences, Universitas Diponegoro, 50275 Tembalang, Semarang, Central Java, Indonesia.
*Corresponding Author
Published In:
Volume - 19,
Issue - 1,
Year - 2026
ABSTRACT:
Sargassum polycystum is a species of brown algae that contains carotenoid compounds with the main content being fucoxanthin. Fucoxanthin is an orange pigment found in Sargassum polycystum. The active fraction of fucoxanthin has antibacterial activity at contents of 500 and 1000 µg/disk against Staphylococcus aureus. Staphylococcus aureus bacteria are gram-positive bacteria that can cause acne vulgaris. The purpose of this study was to determine the appropriate concentrations of fucoxanthin required to prevent Staphylococcus aureus growth, and its formulation into nanogel preparations with active antibacterial properties. The impact of different fucoxanthin concentrations in nanogel preparations on the inhibition of Staphylococcus aureus growth was also evaluated. In this study, the maceration method was used to extract fucoxanthin from fresh Sargassum polycystum. This process was then followed by separation with a column chromatography method, purity testing, as well as using UV-Vis spectrophotometry for identification. Identification of fucoxanthin isolates was carried out using TLC, UV-Vis spectrophotometer, and FTIR spectrophotometer. The formulation was then developed into a nanogel, which underwent testing for transmittance, organoleptic properties, homogeneity, pH, viscosity, spreadability, adhesiveness, and antibacterial activity against Staphylococcus aureus. Fucoxanthin had antibacterial activity at varying concentration, namely 0.1%, 0.2%, and 0.3%, both in its raw form and in the nanogel preparations. Furthermore, there were significant differences (p<0.05) in the growth inhibition of Staphylococcus aureus and in the nanogel physical characteristics, including pH, viscosity, spreadability, and adhesiveness, at different fucoxanthin concentration. Fucoxanthin nanogel showed a good potential to be utilized as an anti-acne product.
Cite this article:
Lia Kusmita, Marshanda Amelia, I Kadek Bagiana, Aninditia Sabdaningsih. Research Journal of Pharmacy and Technology. 2026;19(1):153-9. doi: 10.52711/0974-360X.2026.00024
Cite(Electronic):
Lia Kusmita, Marshanda Amelia, I Kadek Bagiana, Aninditia Sabdaningsih. Research Journal of Pharmacy and Technology. 2026;19(1):153-9. doi: 10.52711/0974-360X.2026.00024 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2026-19-1-24
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