ABSTRACT:
Introduction: Jeevantyadi Avaleha is a polyherbal formulation comprising herbal drugs such as Jeevanti, Satavari, Atibala, Arjuna, Amalaki, Guduchi, and Draksha, along with Sita, and is administered during pregnancy to support healthy foetal development and promote maternal well-being. The term ‘Saviryata Avadhi’ is mentioned in Ayurveda classical texts to denote the shelf life of a drug or the period up to which the drug remains stable and retains its therapeutic potential, unaltered by environmental and microbial contamination. The assessment of the stability of Jeevantyadi Avaleha was concentrated on microbial contamination after the preparation and storage of the drug, considering various weather and temperature conditions. The study aimed to determine the shelf life of the drug, focusing on the impact of temperature and humidity on microbial growth during different periods. Materials and Methods: Three separate batches of Jeevantyadi Avaleha were prepared in the Pharmacy unit of Institute of Teaching and Research in Ayurveda (I.T.R.A), Jamnagar, Gujarat. A microbial study was conducted at regular intervals from the Microbiology Laboratory of Institute of Teaching and Research in Ayurveda (I.T.R.A), Jamnagar, Gujarat. Results: Assessment of microbial contamination was conducted using tests such as wet mount examination, gram staining, fungal culture, and aerobic culture at monthly intervals from July 05, 2021, to January 31, 2023. The highest relative humidity was 95%, and the lowest was 20%. The samples consistently showed no signs of microbial proliferation throughout the study. Discussion: Adherence to recommended storage guidelines and the confirmed absence of microbial contamination validate the safety of Jeevantyadi Avaleha in alignment with the regulatory norms outlined in the Gazette of India. Conclusion: These results confirm that Jeevantyadi Avaleha maintains an acceptable shelf life, which is essential for preserving its therapeutic efficacy and safety during pregnancy.