Author(s):
Khushboo Kushwaha, Anushka Joshi, Krishna Kant Jangde, Rishabh Kumar, Satish Dubey, Kundan Kumar Ojha, Dinesh Kumar Mishra, Ashwini Kumar Dixit
Email(s):
kushwahakhushboo0595@gmail.com , anushkajoshi5321@gmail.com , kjangde10@gmail.com , rishabhkumarbehra1995@gmail.com , satishdubey549@gmail.com , rukundan.ojha790@gmail.com , dineshdops@yahoo.com , dixitak@live.com
DOI:
10.52711/0974-360X.2026.00679 
Address:
Khushboo Kushwaha1, Anushka Joshi1, Krishna Kant Jangde2, Rishabh Kumar3, Satish Dubey4, Kundan Kumar Ojha1, Dinesh Kumar Mishra2, Ashwini Kumar Dixit1
1Department of Botany, Guru Ghasidas Vishwavidyalaya (A Central University), Koni, Bilaspur, Chhattisgarh, India, 495009.
2Department of Pharmacy, Guru Ghasidas Vishwavidyalaya (A Central University), Koni, Bilaspur, C.G., India, 495009.
3Department of Rural Technology, Guru Ghasidas Vishwavidyalaya (A Central University), Koni, Bilaspur, C.G., India, 495009.
4Department of Botany, Dr. Indrajeet Singh Govt. College, Akaltara, Janjgir-Champa, Chhattisgarh, India, 495552.
*Corresponding Author
Published In:
Volume - 19,
Issue - 10,
Year - 2026
ABSTRACT:
Soymida febrifuga (Roxb.) A. Juss., a wild perennial woody plant, is utilized by local communities for a variety of medicinal purposes. To the best of our knowledge, this study is the first to report the a-amylase and a-glucosidase inhibitory potential of the aqueous bark extract of Soymida febrifuga extract (SFE), together with Ultra High-Performance Liquid Chromatography merged with High-Resolution Tandem Mass Spectrometry (UHPLC-Orbitrap HRMS/MS) metabolite profiling. S. febrifuga bark collected from Bilaspur (India) was Soxhlet-extracted with water. Preliminary phytochemical screening and UHPLC-Orbitrap HRMS/MS analysis were performed. In vitro assays of DPPH, a-amylase, a-glucosidase inhibition, and FRAP assays were conducted. SFE showed a 34.56% yield with rich phytochemicals, including saponins, glycosides, and phenolics. HRMS/MS tentatively identified 25 major metabolites such as quinic acid, catechin, and luteolin. The extract demonstrated the inhibition of a-amylase (IC50 547.06 µg/mL) and a-glucosidase (IC50 562.47 µg/mL). Strong antioxidant activity was observed in DPPH (IC50 0.71 µg/mL) and FRAP assays. Significant dose-dependent effects were confirmed (p<0.0001). SFE exhibits strong antioxidant and moderate enzyme inhibitory activities, suggesting a synergistic, multi-metabolite mechanism. Further, the in vivo and mechanistic studies need to be validated for their therapeutic potential.
Cite this article:
Khushboo Kushwaha, Anushka Joshi, Krishna Kant Jangde, Rishabh Kumar, Satish Dubey, Kundan Kumar Ojha, Dinesh Kumar Mishra, Ashwini Kumar Dixit. Antidiabetic and Antioxidant Potential of Soymida febrifuga Aqueous Bark Extract: Enzyme Inhibitory Activity and UHPLC-Orbitrap HRMS/MS Profiling. Research Journal Pharmacy and Technology. 2026;19(10):4867-6. doi: 10.52711/0974-360X.2026.00679
Cite(Electronic):
Khushboo Kushwaha, Anushka Joshi, Krishna Kant Jangde, Rishabh Kumar, Satish Dubey, Kundan Kumar Ojha, Dinesh Kumar Mishra, Ashwini Kumar Dixit. Antidiabetic and Antioxidant Potential of Soymida febrifuga Aqueous Bark Extract: Enzyme Inhibitory Activity and UHPLC-Orbitrap HRMS/MS Profiling. Research Journal Pharmacy and Technology. 2026;19(10):4867-6. doi: 10.52711/0974-360X.2026.00679 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2026-19-10-55
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