Author(s): Khairan Khairan, Syaifullah Muhammad, Nadia Isnaini, Meutia Faradilla, Friesca Erwan, Elly Sufriadi, Binawati Ginting, Suraiya Kamaruzzaman, Adinda Gusti Vonna, Ernawati, Indra Zainun, Hizir Sofyan

Email(s): syaiful.muhammad@usk.ac.id

DOI: 10.52711/0974-360X.2026.00150   

Address: Khairan Khairan, Syaifullah Muhammad*, Nadia Isnaini, Meutia Faradilla, Friesca Erwan, Elly Sufriadi, Binawati Ginting, Suraiya Kamaruzzaman, Adinda Gusti Vonna, Ernawati, Indra Zainun, Hizir Sofyan
Universitas Syiah Kuala, JL. Syech Abdurrauf No.3, Kopelma Darussalam, Kec. Syiah Kuala, Kota Banda Aceh, Aceh, Indonesia, 23111.
*Corresponding Author

Published In:   Volume - 19,      Issue - 3,     Year - 2026


ABSTRACT:
Pogostemom cablin Benth is a flowering plants and commonly known as member of the Lamiaceae family. Pogostemom cablin Benth is plant essential oils as source of raw material for the essential-oil industry that are useful for several therapeutics treatment such as pharmaceutical, food industries, and cosmetics. In this study, herbal body lotion have been formulated using a light fraction of patchouli oil as an active ingredient. The aim of this study was to formulate and evaluate of antioxidant and antiaging activity of body lotion containing light fraction of patchouli oil. The cycling assay method showed that the spread ability; pH; homogeneity; odor, and colour of the lotions were suitable for herbal body lotion. The contaminations assay showed the formulated lotion showed did not contain any heavy metals and microbial contaminations. The antioxidant assay by DPPH method showed that Formula 1 (F1) showed a good antioxidant activity with the IC50 value of 92.50 ppm. Meanwhile, the antiaging activity by MTT assay method showed that Formula 1 (F1) showed low cytotoxic activity against NIH3T3 fibroblast normal cells. Overall, the formulated body lotions showed excellent antioxidant and antiaging activities.


Cite this article:
Khairan Khairan, Syaifullah Muhammad, Nadia Isnaini, Meutia Faradilla, Friesca Erwan, Elly Sufriadi, Binawati Ginting, Suraiya Kamaruzzaman, Adinda Gusti Vonna, Ernawati, Indra Zainun, Hizir Sofyan. Formulation and Evaluation of Antioxidant and Antiaging Activity of the Body Lotion containing Light Fraction of Patchouli Oil. Research Journal Pharmacy and Technology. 2026;19(3):1059-9. doi: 10.52711/0974-360X.2026.00150

Cite(Electronic):
Khairan Khairan, Syaifullah Muhammad, Nadia Isnaini, Meutia Faradilla, Friesca Erwan, Elly Sufriadi, Binawati Ginting, Suraiya Kamaruzzaman, Adinda Gusti Vonna, Ernawati, Indra Zainun, Hizir Sofyan. Formulation and Evaluation of Antioxidant and Antiaging Activity of the Body Lotion containing Light Fraction of Patchouli Oil. Research Journal Pharmacy and Technology. 2026;19(3):1059-9. doi: 10.52711/0974-360X.2026.00150   Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2026-19-3-11


REFERENCES:
1.    Khairan; Muhammad, S.; Diah, M. Patchouli (Pogostemon cablin Benth): Chemistry, Biology, and Anti-Inflammatory Activities: A Review. In Proceedings of the Proceedings of the 2nd International Conference of Essential Oils; SCITEPRESS - Science and Technology Publications, 2019; pp. 67–73.
2.    Ramya, H.G. Study on Steam Distillation of Patchouli (Pogostemon cablin Benth.) and Utilization of the By-Product Spent Leaves, University of Agricultural Sciences, GKVK, Bangalore, India, 2010.
3.    Verma, R.S. Distillate Water: Overlooked Golden Drops. Med. Aromat. Plants 2012, 01, doi:10.4172/2167-0412.1000e112.
4.    Chomchalow, N. Production of Aromatic Plants in Asia-An Overview; Bangkok, 2002;
5.    Janovská, D.; Kubíková, K.; Kokoška, L. Screening for Antimicrobial Activity of Some Medicinal Plants Species of Traditional Chinese Medicine. Czech J. Food Sci. 2011, 21, 107–110, doi:10.17221/3485-CJFS.
6.    Hussain, A.I.; Anwar, F.; Nigam, P.S.; Sarker, S.D.; Moore, J.E.; Rao, J.R.; Mazumdar, A. Antibacterial Activity of Some Lamiaceae Essential Oils Using Resazurin as an Indicator of Cell Growth. LWT - Food Sci. Technol. 2011, 44, 1199–1206, doi:10.1016/j.lwt.2010.10.005.
7.    Hussin, N.; Mondello, L.; Costa, R.; Dugo, P.; Nik Yusoff, N.I.; Yarmo, M.A.; Ab.Wahabd, A.; Said, M. Quantitative and Physical Evaluation of Patchouli Essential Oils Obtained from Different Sources of Pogostemon cablin. Nat. Prod. Commun. 2012, 7, 927–930, doi:10.1177/1934578x1200700732.
8.    Kim, H.W.; Cho, S.J.; Kim, B.-Y.; Cho, S.I.; Kim, Y.K. Pogostemon Cablin as ROS Scavenger in Oxidant-Induced Cell Death of Human Neuroglioma Cells. Evidence-Based Complement. Altern. Med. 2010, 7, 239–247, doi:10.1093/ecam/nem176.
9.    Hasegawa, Y.; Tajima, K.; Toi, N.; Sugimura, Y. An Additional Constituent Occurring in the Oil from a Patchouli Cultivar. Flavour Fragr. J. 1992, 7, 333–335, doi:10.1002/ffj.2730070608.
10.    Lianhua, W.; Yougen, W.; Qiaosheng, G.; Shaopeng, L.; Kaibing, Z.; Junfeng, Z. Comparison of Genetic Diversity in Pogostemon cablin from China Revealed by RAPD, Morphological and Chemical Analyses. J. Med. Plants Res. 2011, 5, 4549–4559.
11.    Swamy, M.; Sinniah, U. A Comprehensive Review on the Phytochemical Constituents and Pharmacological Activities of Pogostemon cablin Benth.: An Aromatic Medicinal Plant of Industrial Importance. Molecules 2015, 20, 8521–8547, doi:10.3390/molecules20058521.
12.    Astuti, P.; Khairan, K.; Marthoenis, M.; Hasballah, K. Antidepressant-like Activity of Patchouli Oil Var. Tapak Tuan (Pogostemon cablin Benth) via Elevated Dopamine Level: A Study Using Rat Model. Pharmaceuticals 2022, 15, 608, doi:10.3390/ph15050608.
13.    Chakrapani, P.; Venkatesh, K.; Chandra, S.S..; Arun Jyothi, B.; Prem, K.; Amareshwari, P.; A, R.R. Phytochemical, Pharmacological Importance of Patchouli (Pogostemon cablin (Blanco) Benth) an Aromatic Medicinal Plant. Int. J. Pharm. Sci. Rev. Res. 2013, 21, 7–15.
14.    Priya, D.; Swati, D.; Vilasrao, D, K. A Review on Pogostemon Patchouli. Res. J. Pharmacogn. Phytochem 2014, 691, 41–47.
15.    Li, P.-B.; Ma, Y.; Wang, Y.-G.; Su, W.-W. Experimental studies on antitussive, expectorant and antiasthmatic effects of  extract from Citrus grandis var. tomentosa.  China J. Chinese  Mater. medica 2006, 31, 1350–1352.
16.    Astuti, R.D.; Safitri, N.; Risdayanti; Khotimah, S. A Stick Formulation of a Mixture of Citronella Oil, Cananga Oil and Patchouli Oil as Aromatherapy. In Proceedings of the In Proceedings of the First International Conference on Health, Social Sciences and Technology; 2021.
17.    Chen, Y.; Wu, Y.-G.; Xu, Y.; Zhang, J.-F.; Song, X.-Q.; Zhu, G.-P.; Hu, X.-W. Dynamic Accumulation of Sesquiterpenes in Essential Oil of Pogostemon cablin. Rev. Bras. Farmacogn. 2014, 24, 626–634, doi:10.1016/j.bjp.2014.11.001.
18.    Nikiforov, A.; Jirovetz, L.; Buchbauer, G.; Raverdino, V. GC-FTIR and GC-MS in Odour Analysis of Essential Oils. Mikrochim. Acta 1988, 95, 193–198, doi:10.1007/BF01349751.
19.    Neerman, M. Sesquiterpene Lactones: A Diverse Class of Compounds Found in Essential Oils Possessing Antibacterial and Antifungal Properties. Int. J. Aromather. 2003, 13, 114–120, doi:10.1016/S0962-4562(03)00078-X.
20.    Tohidi, B.; Rahimmalek, M.; Arzani, A. Essential Oil Composition, Total Phenolic, Flavonoid Contents, and Antioxidant Activity of Thymus Species Collected from Different Regions of Iran. Food Chem. 2017, 220, 153–161, doi:10.1016/j.foodchem.2016.09.203.
21.    Liu, G.; Slater, N.; Perkins, A. Epilepsy: Treatment Options. Am. Fam. Physician 2017, 96, 87–96.
22.    Xu, N.; Luo, H.; Li, M.; Wu, J.; Wu, X.; Chen, L.; Gan, Y.; Guan, F.; Li, M.; Su, Z.; et al. β-Patchoulene Improves Lipid Metabolism to Alleviate Non-Alcoholic Fatty Liver Disease via Activating AMPK Signaling Pathway. Biomed. Pharmacother. 2021, 134, 111104, doi:10.1016/j.biopha.2020.111104.
23.    Donelian, A.; Carlson, L.H.C.; Lopes, T.J.; Machado, R.A.F. Comparison of Extraction of Patchouli (Pogostemon cablin) Essential Oil with Supercritical CO2 and by Steam Distillation. J. Supercrit. Fluids 2009, 48, 15–20, doi:10.1016/j.supflu.2008.09.020.
24.    van Beek, T.A.; Joulain, D. The Essential Oil of Patchouli, Pogostemon cablin : A Review. Flavour Fragr. J. 2018, 33, 6–51, doi:10.1002/ffj.3418.
25.    Nurhadianty, V.; Amalia Amri, I.; Kanza, S.; Putu Maharani, L.; Cahyani, C. Moisturizing Lotion Formulation on Tropical Skin Based on Cananga Oil (Cananga odorata), Kaffir Lime Oil (Citrus hystrix DC) and Patchouli Oil (Pogostemon cablin) as a Bioactive. In Proceedings of the Proceedings of the 2nd International Conference of Essential Oils; SCITEPRESS - Science and Technology Publications, 2019; pp. 106–111.
26.    Zineb, M.; Zoubida, M.; Kamilia, B.; Fateh, S. Socioeconomic valorization and development of a bio-fungicide from essential oils of four Algerian aromatic and medicinal plants: Artemisia herba alba Asso, Mentha pulegium L, Rosmarinus officinalis L and Ocimum basilicum L. Asian J. Research Chem. 2020; 13(6): 473-484. doi: 10.5958/0974-4150.2020.00084.X.
27.    Monika, G.; Aditi, G.;, Sudhakar, G. Insecticidal Activity of Essential Oils Obtained from Piper nigrum and Psoralea corylifolia Seeds against Agricultural Pests. Asian J. Research Chem. 2013, 6(4); 360-363.
28.    Naima, B.; Segni, L.; Mohamed, B.G.; Amar, E.; Kifah, W.M.S. Chemical compositions, Fumigant and Repellent Activities, of Essential oils from three Indigenous medicinal plants and their mixture, against stored grain pest, Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae). Asian J. Research Chem. 2020; 13(6): 455-464. doi: 10.5958/0974-4150.2020.00081.4.
29.    Chasanah, U.; Ermawati, D. Lotion Formulation Combination of Cananga and Patchouli Oil as a Repellent. In Proceedings of the International Conference on Bioinformatics and Nano-medicine from Natural Resources for Biomedical Research; AIP Publishing, 2019; p. 020018.
30.    Febriyenti, Netty. S.; Rizki F.P. Formulation and Evaluation of Patchouli Oil Microemulsion and Microemulgel for Inhibit the Bacterial Growth. Research Journal of Pharmacy and Technology. 2022; 15(1): 51-5. doi: 10.52711/0974-360X.2022.00010.
31.    Aman. Y.; Dinesh, M.P.; Pritesh, P.; Nadeem. F.; Arpit .G. Formulation and Evaluation of Polyherbal Antiaging Cream. Asian Journal of Pharmacy and Technology. 2021; 11(4): 284-8. doi: 10.52711/2231-5713.2021.00047.
32.    Kabita, B.; Simhachalam, R.; Gupta, B.K. Chromatographic Estimation of Niacinamide in Anti aging Dermatological product. Asian J. Pharm. Ana. 2019; 9(2): 62-66. doi: 10.5958/2231-5675.2019.00013.9.
33.    Karamać, M.; Profile, S.; Bucinski, A. Antioxidant and Antiradical Activity of Ferulates Neuropsychology of Somatic Disorders View Project The Importance of Walnut and the Effective Utilization of Its by Products View Project. Artic. Czech J. Food Sci. 2005. doi:10.17221/3373-CJFS.
34.    Torres-Martínez, R.; García-Rodríguez, Y.M.; Ríos-Chávez, P.; Saavedra-Molina, A.; López-Meza, J.E.; Ochoa-Zarzosa, A.; Garciglia, R.S. Antioxidant Activity of the Essential Oil and Its Major Terpenes of Satureja macrostema (Moc. and Sessé Ex Benth.) Briq. Pharmacogn. Mag. 2018, 13, S875–S880, doi:10.4103/pm.pm_316_17.
35.    Stankovic, M.S.; Niciforovic, N.; Mihailovic, V.; Topuzovic, M.; Solujic, S. Antioxidant Activity, Total Phenolic Content and Flavonoid Concentrations of Different Plant Parts of Teucrium polium L. Subsp. Polium. Acta Soc. Bot. Pol. 2012, 81, 117–122, doi:10.5586/asbp.2012.010.
36.    Dolorosa, M.T.; Nurjanah; Purwaningsih, S.; Anwar, E. Utilization of Kappaphycus alvarezii and Sargassum plagyophyllum from Banten as Cosmetic Creams. IOP Conf. Ser. Earth Environ. Sci. 2020, 404, 012008, doi:10.1088/1755-1315/404/1/012008.
37.    Asha, S.; Thirunavukkarasu, P.; Mani, V.; Sadiq, A. Antioxidant Activity of Euphorbia hirta Linn Leaves Extracts. European J. Med. Plants 2016, 14, 1–14, doi:10.9734/EJMP/2016/24952.
38.    Soh, M.; Kang, D.-W.; Jeong, H.-G.; Kim, D.; Kim, D.Y.; Yang, W.; Song, C.; Baik, S.; Choi, I.-Y.; Ki, S.-K.; et al. Ceria-Zirconia Nanoparticles as an Enhanced Multi-Antioxidant for Sepsis Treatment. Angew. Chemie Int. Ed. 2017, 56, 11399–11403, doi:10.1002/anie.201704904.
39.    Arifan, F.; Winarni, S.; Wahyuningsih, W.; Pudjihastuti, I.; Broto, R.W. Total Plate Count (TPC) Analysis of Processed Ginger on Tlogowungu Village. In Proceedings of the Proceedings of the International Conference on Maritime and Archipelago (ICoMA 2018); Atlantis Press: Paris, France, 2019.
40.    Mosmann, T. Rapid Colorimetric Assay for Cellular Growth and Survival: Application to Proliferation and Cytotoxicity Assays. J. Immunol. Methods 1983, 65, 55–63, doi:10.1016/0022-1759(83)90303-4.
41.    Sieuwerts, A.M.; Klijn, J.G.M.; Peters, H.A.; Foekens, J.A. The MTT Tetrazolium Salt Assay Scrutinized: How to Use This Assay Reliably to Measure Metabolie Activity of Cell Cultures in Vitro for the Assessment of Growth Characteristics, IC50-Values and Cell Survival. Clin. Chem. Lab. Med. 1995, 33, doi:10.1515/cclm.1995.33.11.813.
42.    Muntafiah, L.; Shabrina, B.A.; Sulistyowati, D.; Asshagab, M.R.N.; Jenie, R.I. Anti-Aging Activity of Cucurbita moschata Ethanolic Extract Towards NIH3T3 Fibroblast Cells Induced By Doxorubicin. Indones. J. Cancer Chemoprevention 2017, 7, 49, doi:10.14499/indonesianjcanchemoprev7iss2pp49-53.
43.    Misharina, T.A.; Terenina, M.B.; Krikunova, N.I. Antioxidant Properties of Essential Oils. Appl. Biochem. Microbiol. 2009, 45, 642–647, doi:10.1134/S000368380906012X.
44.    Soon, H.S.; Shuchi, A.; Sundaramurthy, J.; Ming, T.T.; Cindy, L.Y.L.; Akshay, J. A Study of Essential Oil Extraction and Antioxidant Activity of Patchouli (Pogostemon cablin) Using Supercritical Carbon Dioxide. ON12 2018.
45.    Musdalipah, M.; Tee, S.A.; Karmilah, K.; Sahidin, S.; Fristiohady, A.; Yodha, A.W.M. Total Phenolic and Flavonoid Content, Antioxidant, and Toxicity Test with BSLT of Meistera chinensis Fruit Fraction from Southeast Sulawesi. Borneo J. Pharm. 2021, 4, 6–15, doi:10.33084/bjop.v4i1.1686.
46.    Dechayont, B.; Ruamdee, P.; Poonnaimuang, S.; Mokmued, K.; Chunthorng-Orn, J. Antioxidant and Antimicrobial Activities of Pogostemon cablin (Blanco) Benth. J. Bot. 2017, 2017, 1–6, doi:10.1155/2017/8310275.
47.    Tahir, M.; Muflihunna, A.; Syafrianti, S. Penentuan Kadar Fenolik Total Ekstrak Etanol Daun Nilam (Pogostemon cablin Benth.) Dengan Metode Spektrofotometri Uv-Vis. J. Fitofarmaka Indones. 2017, 4, 215–218, doi:10.33096/jffi.v4i1.231.
48.    Suresh, S.; Jayamoorthy, K.; Saravanan, P.; Karthikeyan, S. Switch-Off Fluorescence of 5-Amino-2-Mercapto Benzimidazole with Ag3O4 Nanoparticles: Experimental and Theoretical Investigations. Sensors Actuators B Chem. 2016, 225, 463–468, doi:10.1016/j.snb.2015.11.056.
49.    Barrett, A.H.; Farhadi, N.F.; Smith, T.J. Slowing Starch Digestion and Inhibiting Digestive Enzyme Activity Using Plant Flavanols/Tannins-A Review of Efficacy and Mechanisms. LWT. 2018, 87, 394–399, doi:10.1016/j.lwt.2017.09.002.
50.    Thummajitsakul, S.; Samaikam, S.; Tacha, S.; Silprasit, K. Study on FTIR Spectroscopy, Total Phenolic Content, Antioxidant Activity and Anti-Amylase Activity of Extracts and Different Tea Forms of Garcinia schomburgkiana Leaves. LWT 2020, 134, 110005, doi:10.1016/j.lwt.2020.110005.
51.    Kusuma, H.S.; Mahfud, M. Microwave-Assisted Hydrodistillation for Extraction of Essential Oil from Patchouli (Pogostemon cablin) Leaves. Period. Polytech. Chem. Eng. 2016, doi:10.3311/PPch.8676.
52.    Olivier, R.; Emile, M.; Gaydou, Robert, F.; Isabelle, B. (1997) Sesquiterpenes from Patchouli (Pogostemon cablin) Essential Oil. Assignment of the Proton and Carbon-13 NMR Spectra, Journal of Essential Oil Research. 1997. 9:3, 321-327, DOI: 10.1080/10412905.1997.10554251.
53.    Thomas, S.; Ernest, B.; Kevin, B.; Aubin, N.L.; Pierre, C. Aromatic Plants from “Plateau des Cataractes”: Kinetic modeling of the extraction of leaf essential oils from Curcuma mangga (Valeton and Zijp) acclimatized in Congo-Brazzaville. Asian Journal of Research in Chemistry. 2021; 14(3): 186-4. doi: 10.52711/0974-4150.2021.00034.
54.    Ansel, H.C.; Popovich; Allen, L. V Pharmaceutical Dosage Forms and Drug Delivery System (Sixth Edition); Lippincott Williams and Wilkins: Philadelphia, 1989;
55.    Helal, D..; Abdel-rehman, A.; Abdel-halim, S.; Nabarawi, M.. Formulation and Evaluation of Fluconazole Topical Gel. Int. J. Pharm. Pharm 2012, 4, 176–183.
56.    Hana, R.B.; Sawsan, A.; Oran, Y.K.; Bustanji. Chemical Composition and Antiproliferative and Antioxidant Activities of Essential Oil from Juniperus phoenicea L. Cupressaceae. Research Journal of Pharmacy and Technology. 2022; 15(1):153-9. doi: 10.52711/0974-360X.2022.00025.
57.    Burali, S.; Kulkarni, A.R. Antioxidant and Anti-colon cancer activity of fruit peel of Citrus reticulate essential oil on HT-29 cell line. Research J. Pharm. and Tech. 6(2): Feb. 2013; Page 216-219.
58.    Ali Khan, B.; Ullah, S.; Khan, M.K.; Alshahrani, S.M.; Braga, V.A. Formulation and Evaluation of Ocimum basilicum-Based Emulgel for Wound Healing Using Animal Model. Saudi Pharm. J. 2020, 28, 1842–1850, doi:10.1016/j.jsps.2020.11.011.
59.    Hussain, A.; Anwar, F.; Iqbal, T.; Bhatti, I. Antioxidants Attributes of Four Lamiaceae Essential Oils. Pakistan J. Bot. 2011, 43, 1315–1321.
60.    Rahmi, D.; Yunilawati, R.; Jati, B.N.; Setiawati, I.; Riyanto, A.; Batubara, I.; Astuti, R.I. Antiaging and Skin Irritation Potential of Four Main Indonesian Essential Oils. Cosmetics 2021, 8, 94, doi:10.3390/cosmetics8040094.

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