Author(s):
Nashwa Athaya Admiral, Febriyenti, Regina Andayani
Email(s):
uniregina74@gmail.com
DOI:
10.52711/0974-360X.2026.00370 
Address:
Nashwa Athaya Admiral, Febriyenti, Regina Andayani*
Faculty of Pharmacy, Andalas University, Kampus Limau Manis, Padang 25163, West Sumatera, Indonesia.
*Corresponding Author
Published In:
Volume - 19,
Issue - 6,
Year - 2026
ABSTRACT:
Red ginger rhizome extract (Zingiber officinale var. rubrum) exhibits pharmacological activities including anti-inflammatory, analgesic, antioxidant, and anti-emetic effects, indicating its potential for development into herbal pharmaceutical preparation such as Orally Disintegrating Films (ODFs). ODFs were selected for their efficient administration. This study developed and validated a TLC-densitometry method for the analysis of 6-shogaol as a marker compound to ensure the quality of red ginger extract and ODFs preparation. Although red ginger extract has been formulated into ODFs, a practical method for quantifying the extract's concentration has not been previously established. Optimization of the mobile phase using a silica gel F254 TLC plate identified n-hexane: ethyl acetate (9:1) as optimal, yielding an Rf value of 0.37±0.03, Rs 1.6, TF 1, JSPT 9.13075×10-5, and N 87616. Analysis of 6-shogaol was performed at a wavelength of 279nm. Method validation demonstrated linearity with a correlation coefficient of 0.9978. The limits of detection and quantitation were 35.97µg/mL and 119.91µg/mL, respectively. Intra-day and inter-day precision ranged from 0.24% to 0.71% and 0.49% to 0.57%, respectively. Accuracy testing showed recovery percentages in the extract of 101.02% to 102.77% and in the ODFs of 100.36% to 102.49%. These results confirm that the TLC-densitometry method is valid for analyzing 6-shogaol in both the extract and ODFs of Zingiber officinale var. rubrum.
Cite this article:
Nashwa Athaya Admiral, Febriyenti, Regina Andayani. Optimization and Validation of TLC Densitometry Method for 6-shogaol Analysis in Orally Disintegrating Films (ODFs) of Standardized Ginger (Zingiber officinale var. rubrum) extract. Research Journal Pharmacy and Technology. 2026;19(6):2585-2. doi: 10.52711/0974-360X.2026.00370
Cite(Electronic):
Nashwa Athaya Admiral, Febriyenti, Regina Andayani. Optimization and Validation of TLC Densitometry Method for 6-shogaol Analysis in Orally Disintegrating Films (ODFs) of Standardized Ginger (Zingiber officinale var. rubrum) extract. Research Journal Pharmacy and Technology. 2026;19(6):2585-2. doi: 10.52711/0974-360X.2026.00370 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2026-19-6-27
REFERENCES:
1. Putri M. Khasiat dan Manfaat Jahe Merah. Semarang: ALPRIN. 2019.
2. BPS. Produksi Tanaman Biofarmaka. 2020.
3. Anggraeni R, Aljaberi M. Pharmaceutical effect of Red Ginger (Zingiber officinale var. rubrum) on Arthritis and Gout pain in Older people at Parungkuda Public Health Center’s Geriatric Polyclinic in Sukabumi Regency, Indonesia. Res J Pharm Technol. 2023; 16(5): 2115–2119.
4. Widyaningsih T, Sari A, Rukmi W. The Effect of Moringa Leaf (Moringa oleifera Lam.), Pandan Wangi (Pandanus amaryllifolius Roxb.) and Red Ginger (Zingiber officinale Rosc.Var) Extract Supplement Intervention on Uric Acid, Kidney, Liver Function and Perceptions of Hyperuricemia patients o. Res J Pharm Technol. 2022; 15(10): 4474–4477.
5. Kandhan T, Roy A, Rajeshkumar T. Green Synthesis of Rosemary Oleoresin Mediated Silver Nanoparticles and its Effect on Oral Pathogens. Res J Pharm Tech. 2019; 12(11): 5379–5382.
6. Irfan M, Rabel S, Bukhtar Q, Qadi M, Jabeen F, Khan A. Orally Disintegrating Films: A Modern Expansion in Drug Delivery System. Saudi Pharm J. 2016; 24(5): 537–546.
7. Nandi S, Das G, Reza K. Formulation, Evaluation and Optimization of Orally Disintegrating Films of Azithromycin Using DOE Approach for the Pediatrics with Acute Otitis Media. Res J Pharm Technol. 2022; 15(11): 5031–5037.
8. Mazumder S, Pavurala N, Mand P, Xu X, Cruz C, Krishnaiah Y. Quality by Design Approach for Studying the Impact of Formulation and Process Variables on Product Quality of Orally Disintegrating Films. Int J Pharm. 2017; 527(1–2): 151–160.
9. Amin P, Gangurde A, Alai P. Oral Film Technology : Challenges and Future Scope for Pharmaceutical Industry. Int J Pharm Pharm Res. 2015; 3(3): 183–203.
10. Febriyenti, Rizki SA, Intan R, Azizah M, Syahyuning KC, Meri S. Orally Disintegrating Film Formulation Extract of Red Ginger (Zingiber officinale var. rubrum). Drug Discovery. 2025;19:1-9.
11. Alqasoumi S. Quantification of 6-gingerol in Zingiber officinale Extract, Ginger-containing Dietary Supplements, Teas, and Commercial Creams by Validated HPTLC Densitometry. FABAD J. Pharm. Sci. 2009; 34: 33-42.
12. Yusufoglu H, Alqasoumi S. High Performance Thin Layer Chromatographic Analysis of 10-gingerol in Zingiber officinale Extract and Ginger-containing Dietary Supplements, Teas, and Commercial Creams. FABAD J. harm. Sci. 2008; 33: 199-204.
13. Srikandi S, Humaeroh M, Sutamihardja R. Kandungan Gingerol Dan Shogaol Dari Ekstrak Jahe Merah (Zingiber Officinale Roscoe) Dengan Metode Maserasi Bertingkat. al-Kimiya. 2020; 7(2): 75–81.
14. Heru Agus Cahyanto. Standardisasi Simplicia Dan Ekstrak Etanol Jahe Merah (Zingiber officinale Rosch. var Rubrum) Dari Lahan Gambut Kubu Raya, Kalimantan Barat. J Borneo Akcaya. 2022; 7(2): 49–55.
15. Sa’diah S, Anwar E, Jufri M, Cahyaningsih U. Perbandingan Ekstrak Jahe Merah (Zingiber officinale var.Rubrum), Gingerol dan Shogaol sebagai Anti-Toksoplasma terhadap Parasit Toxoplasma Gondii Secara In-Vitro. 2019; 4(3): 93–102.
16. Vyas J, Desai U, Patel A, Patel S, Sheth D. A Brief Review on Dual Wavelength Spectrophotometry: The Simultaneous Estimation Method and its Application. Asian J Pharm Anal. 2024;14(3):166–174.
17. Galgatte U. Challenges in formulation development of fast dissolving oral film. Indo Am J Pharm Res. 2014; 3(8): 6391 – 6407.
18. Darusman F, Ramadhan M, Lantika U. Formulasi Dan Karakterisasi Sediaan Orally Dissolving Film Tamsulosin Hidroklorida. J Ilm Farm Farmasyifa. 2023; 6(1): 29–40.
19. Liew K, Gobal G, Rofiq M, Phang C, Lee S, Ming L. Orally Disintegrating Film: A Review of Its Formulation and Manufacturing Method. Malaysian J Med Heal Sci. 2023; 19(6): 297–303.
20. Foudah AI, Shakeel F, Yusufoglu HS, Ross SA, Alam P. Simultaneous Determination of 6-shogaol and 6-gingerol in Various Ginger (Zingiber officinale Roscoe) Extracts and Commercial Formulations Using a Green RP-HPTLC-densitometry Method. Foods. 2020; 9(8): 11–15.
21. Kealey D, Halines P. Instant Notes : Analytical Chemistry. In: BIOS. New York: Scientific Publisher Limited; 2014.
22. Jazokaite R, Marksa M, Zevzikoviene A, Zevzikovas A. Chromatographic Analysis of 6-gingerol and 6-shogaol Using TLC and HPLC Methods. Sci Pharm Sci. 2019; 2019(2): 10–15.
23. Kementrian Kesehatan RI Farmakope Herbal Indonesia. II. Jakarta: Kementerian Kesehatan Republik Indonesia; 2017.
24. Lonare A, Nipurte D, Lonare A. Extraction, Paper and Thin Layer Chromatography and Activity of Curcumin. Asian J Pharm Technol. 2024; 14(1): 13–15.
25. Paun J, Raval M, Sheth N. Optimization of Solvents and Processing Conditions for Crystallization of Aceclofenac. Asian J Res Pharm Sci. 2024; 3(3): 122–132.
26. Shahana V, Ramya G, Rajesh A, Kathirvel S. A Comprehensive Validation Method and Development of RP-HPLC for Simultaneous Estimation of Metoprolol, Telmisartan and Chlorthalidonein Bulk and its Formulation. Asian J Res Chem. 2018; 11(6): 827–834.
27. Takeuchi Y, Hayakawa F, Tahara K. Orally Disintegrating Films: The Effects of Water Content on Disintegration and Mechanical Properties. J Drug Deliv Sci Technol. 2021; 66(1): 1–9.
28. Vyas J, Patel B, Patel A, Patel A, Patel N. A Brief Review on Q-absorption Ratio Method in UV-Spectrophotometry. Asian J Pharm Anal. 2022; 12(4): 281–285.
29. Chemical C. Product Information : 6-Shogaol. United States Of America: Cayman Chemical Company; 2018.
30. Sristava M. High-Performance Thin Layer Chromatography (HPTLC). New York: Springer; 2014.
31. Harmita. Petunjuk Pelaksanaan Validasi Metode Dan Cara Perhitungannya. Maj Ilmu Kefarmasian. 2014; 1(3).
32. AOAC. Official Methods of Analysis of AOAC International. AOAC Int. 2020.