ABSTRACT:
RP-HPLC isocratic method was developed for mangiferin (MAN) in polyherbal liquid, Saptarangi plus Kadha and herbal tablet, Saptarangi tablet (vati) formulation using JASCO (LC4000) System, 4010 diode array detector, YMC-Pack Pro C-18, (250 mm × 4.6 mm, 3 µm) column, software (Crome-Nav 2), data integration at 254 nm was achieved. Water: ACN (30:70 v/v), mobile phase, pH adjusted to 3 with orthophosphoric acid at flow rate of was 1 ml/min was used. The analysis was completed within 6 minutes. Hence, consequently speed and economy has been achieved in analysis. System suitability parameters for analytes were well within the limit. The method was validated as per ICH guidelines and was found to be simple, linear, specific, accurate and robust. The linearity was observed over the concentration range of 10-50 µg/ml at 254 nm with R2 0.995 for MAN. The method precision (inter-day and intra-day) was determined using six -independent test solutions and % RSD was found <2. Assay method was evaluated with the recovery of the standards. The results of recovery found close to 100 % confirms accuracy of the method. The result of precision and recovery shows that the methods is precise and accurate. The values of LOD and LOQ were calculated and method were found to be sensitive. RT of standard in chromatograms of extracts and herbal preparations matches perfectly. The values of purity front and purity tail were found greater than 0.99 for MAN which indicate peak is pure. Hence, a method is specific. The effect of different variations on peak areas was evaluated by calculating %RSD for each condition which indicate low value for %RSD (less than 2) and hence, method is robust.