Author(s):
Manju Bargavi Sakthivel, Prabhavathy Devi Narayanan Doss, Nalini Devarajan, Tharani Munusamy, Suresh Malchi, Naresh Kumar Kodaganti, Karunanidhi Kanappan
Email(s):
prabhavathy_devi@yahoo.com
DOI:
10.52711/0974-360X.2026.00384 
Address:
Manju Bargavi Sakthivel1, Prabhavathy Devi Narayanan Doss1*, Nalini Devarajan2, Tharani Munusamy2, Suresh Malchi2, Naresh Kumar Kodaganti2, Karunanidhi Kanappan2
1Department of Nutrition and Dietetics, Faculty of Humanities and Science, Meenakshi Academy of Higher Education and Research, K.K. Nagar, Chennai-600 078, Tamil Nadu, India.
2Department of Research, Meenakshi Academy of Higher Education and Research, K.K. Nagar, Chennai-600 078, Tamil Nadu, India.
*Corresponding Author
Published In:
Volume - 19,
Issue - 6,
Year - 2026
ABSTRACT:
This research aims to explore the synthesis and multi-bioactive investigation of berberine nanoformulation copper oxide nanoparticles (BNF-CuONPs), targeting antioxidant, antimicrobial and anticancer activities. The synthesized berberine nanoformulation was characterized by UV-visible spectroscopy, Transmission Electron Microscopy, and Scanning Electron Microscopy. UV-visible spectra confirmed the formation of nanoparticles with a distinct absorption peak, while SEM and TEM images demonstrated a spherical morphology with a uniform dispersion of bioactive compounds. The antioxidant properties of the berberine nanoformulation were evidenced by the DPPH assay, with the highest inhibition percentage compared to standards. Furthermore, the antimicrobial assay showed significant inhibitory effects against Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, and Escherichia coli, with dose-response inhibition of zones confirming broad-spectrum antimicrobial activity. For anticancer studies, DMSO was used as a solvent control and exhibited 98% cell viability, validating the specificity of the cytotoxicity induced by the berberine nanoformulation in NCI-H460 cells. The cellular toxicity showed a dose-responsive response at the maximum concentration (200 µg/mL), resulting in 97% cell suppression, indicating selective anticancer activity. In normal cells, viable cells ranged from 99% (20 µg/mL) to 97% (200 µg/mL), indicating almost no toxicity. The cell migration assay revealed a strong anti-migratory effect, observed as changes in wound space and the relative cell migration decreasing to 0.05, indicating anti-metastatic potential. Overall, the findings suggest that berberine nanoformulation of copper oxide nanoparticles exhibited enhanced bioactive compounds suitable for anti-microbial, anti-oxidant, cytotoxicity and cell migration assay.
Cite this article:
Manju Bargavi Sakthivel, Prabhavathy Devi Narayanan Doss, Nalini Devarajan, Tharani Munusamy, Suresh Malchi, Naresh Kumar Kodaganti, Karunanidhi Kanappan. Formulation of Bioactive Berberine–Copper Oxide Nanoparticles via Solanum torvum for Multifunctional Antioxidant, Antimicrobial, Anticancer, and Wound-Healing Applications in NCI-H460 cells. Research Journal Pharmacy and Technology. 2026;19(6):2688-4. doi: 10.52711/0974-360X.2026.00384
Cite(Electronic):
Manju Bargavi Sakthivel, Prabhavathy Devi Narayanan Doss, Nalini Devarajan, Tharani Munusamy, Suresh Malchi, Naresh Kumar Kodaganti, Karunanidhi Kanappan. Formulation of Bioactive Berberine–Copper Oxide Nanoparticles via Solanum torvum for Multifunctional Antioxidant, Antimicrobial, Anticancer, and Wound-Healing Applications in NCI-H460 cells. Research Journal Pharmacy and Technology. 2026;19(6):2688-4. doi: 10.52711/0974-360X.2026.00384 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2026-19-6-41
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