Author(s):
Yosephine Sri Wulan Manuhara, Syamsuardi Syamsuardi, Suprapto Suprapto, Ummi Zubaidah, Anjar Tri Wibowo, Nanik Siti Aminah, Alfinda Novi Kristanti, Sugiharto, Djarot Sugiarso
Email(s):
yosephine-s-w-m@fst.unair.ac.id
DOI:
10.52711/0974-360X.2026.00388 
Address:
Yosephine Sri Wulan Manuhara1,2*, Syamsuardi Syamsuardi3, Suprapto Suprapto4, Ummi Zubaidah1, Anjar Tri Wibowo1,2, Nanik Siti Aminah5,2, Alfinda Novi Kristanti5,2, Sugiharto1,2, Djarot Sugiarso4
1Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Kampus Merr C, Jl. Dr. Ir. H. Soekarno, Mulyorejo, Surabaya 60115, Indonesia.
2Biotechnology of Tropical Medicinal Plants Research Group, Universitas Airlangga, Kampus Merr C, Jl. Dr. Ir. H. Soekarno, Mulyorejo, Surabaya 60115, Indonesia.
3Department of Biology, Faculty of Mathematics and Sciences, Andalas University, Padang, Indonesia.
4Department of Chemistry, Faculty of Science and Data Analytics, Institut Teknologi Sepuluh Nopember, Surabaya, 60111, Indonesia.
5Department of Chemistry, Faculty of Science and Technology, Universitas Airlangga, Kampus Merr C, Jl. Dr. Ir. H. Soekarno, Mulyorejo, Surabaya 60115, Indonesia.
*Corresponding Author
Published In:
Volume - 19,
Issue - 6,
Year - 2026
ABSTRACT:
Recent research has focused on examining the metabolite diversity of U. gambir Roxb. cv. Cubadak to assess its antioxidant and catechin content across various plant organs, processing methods, and extract types. The antioxidant ability is further tested on pathogenic microbes. U. gambir samples were prepared in the forms of catechu-2 (G2), catechu-1 (G1), dried leaves (D), fresh leaves (FL), fresh twigs (FS), and fresh roots (FR). Extractions were performed using methanol, ethyl acetate, and n-hexane. The resulting extracts underwent metabolite profiling via LC-MS/MS, DPPH assay, total phenolic content (TPC), total flavonoid content (TFC), and total catechin content. Antimicrobial testing was performed on Escherichia coli, Staphylococcus aureus and Candida albicans. U. gambir Roxb. cv. Cubadak contains lipids, terpenoids, benzene derivatives, flavonoids, and alkaloids. Both solvent polarity and type of raw material significantly impacted antioxidant activity, TPC, TFC, and total catechin content. Among samples, the best antioxidant activity was observed in the following order: G2 > G1 > D > FL > FS > FR. Pearson correlation analysis confirmed a positive association between high antioxidant capacity and increased levels of TPC, TFC, and total catechin. U. gambir showed antimicrobial activity on fungal pathogen compared to bacteria. Taken together, the combination of G2 processed with ethyl acetate extraction demonstrated superior antioxidant activity (IC50 = 8.82µg/mL), along with high levels of TPC (1420mg GAE/g DW), TFC (1902 mg QE/g DW), and total catechin (784.45µg/mL), highlighting its potential as a natural antioxidant source. This antioxidant exhibited fair antimicrobial.
Cite this article:
Yosephine Sri Wulan Manuhara, Syamsuardi Syamsuardi, Suprapto Suprapto, Ummi Zubaidah, Anjar Tri Wibowo, Nanik Siti Aminah, Alfinda Novi Kristanti, Sugiharto, Djarot Sugiarso. Exploring the Medicinal Potency of Uncaria gambir (W. Hunter) Roxb. Cv. Cubadak: Antioxidant Activity, Catechin Content, Metabolite Profiling, and Antimicrobial Testing as a Basis for Potential Therapeutic Use. Research Journal Pharmacy and Technology. 2026;19(6):2714-2. doi: 10.52711/0974-360X.2026.00388
Cite(Electronic):
Yosephine Sri Wulan Manuhara, Syamsuardi Syamsuardi, Suprapto Suprapto, Ummi Zubaidah, Anjar Tri Wibowo, Nanik Siti Aminah, Alfinda Novi Kristanti, Sugiharto, Djarot Sugiarso. Exploring the Medicinal Potency of Uncaria gambir (W. Hunter) Roxb. Cv. Cubadak: Antioxidant Activity, Catechin Content, Metabolite Profiling, and Antimicrobial Testing as a Basis for Potential Therapeutic Use. Research Journal Pharmacy and Technology. 2026;19(6):2714-2. doi: 10.52711/0974-360X.2026.00388 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2026-19-6-45
REFERENCES:
1. Yeni G, Syamsu K, Suparno O, Mardliyati E, Muchtar H. Repeated Extraction Process of Raw Gambiers (Uncaria gambier Robx.) for the Catechin Production as an Antioxidant. International Journal of Applied Engineering Research. 2014; 9(24).
2. Ho TJ, Tsai PH, Hsieh CH, Lin JH, Lin YW, Wu JR, et al. Role of Herbal Extracts of Catechu from Uncaria gambir in the Treatment of Chronic Diabetic Wounds. Pharmaceuticals. 2022; Dec 31; 16(1): 66. DOI: 10.3390/ph16010066.
3. Munggari IP, Kurnia D, Deawati Y, Julaeha E. Current Research of Phytochemical, Medicinal and Non-Medicinal Uses of Uncaria gambir Roxb.: A Review. Molecules. 2022 Oct 3; 27(19): 6551. DOI: 10.3390/molecules27196551
4. Wardana AP, Aminah NS, Kristanti AN, Fahmi MZ, Zahrah HI, Widiyastuti W, et al. Nano Uncaria gambir as Chemopreventive Agent Against Breast Cancer. Int J Nanomedicine. 2023 Aug; Volume 18: 4471–84. DOI: 10.2147/IJN.S403385
5. Yunarto N, Sulistyowati I, Reswandaru UN, Elya B, Sauriasari R, Konadi L. Inhibitory activity of Uncaria Gambir Roxb extract, ethyl acetate fraction, and catechin isolate on lipase. In 2023. p. 020011. DOI: 10.1063/5.0118363
6. Zebua EA, Silalahi J, Julianti E. Hypoglicemic activity of gambier (Uncaria gambir robx.) drinks in alloxan-induced mice. IOP Conf Ser Earth Environ Sci. 2018 Feb; 122: 012088.
7. Abral H, Kurniawan A, Rahmadiawan D, Handayani D, Sugiarti E, Muslimin AN. Highly antimicrobial and strong cellulose-based biocomposite film prepared with bacterial cellulose powders, Uncaria gambir, and ultrasonication treatment. Int J Biol Macromol. 2022 May; 208: 88–96. DOI: 10.1016/j.ijbiomac.2022.02.154.
8. Anggraini T, Tai A, Yoshino T, Itani T. Antioxidative activity and catechin content of four kinds of Uncaria gambir extracts from West Sumatra, Indonesia. African Journal of Biochemistry Research. 2011; 5(1): 33–8.
9. Sugiharto, Darmanto W, Wahyuningsih SPA, Hayati A, Martha Y, Olivia P, et al. Antioxidant Activities of Curcumin to MDA Blood Serum Concentration and Lead Levels in Liver of Mice. Malaysian Journal of Science. 2019 Nov 30; 38: 21–9.
10. Kaewseejan N, Siriamornpun S. Bioactive components and properties of ethanolic extract and its fractions from Gynura procumbens leaves. Ind Crops Prod. 2015; 74: 271–8. DOI: https://doi.org/10.1016/j.indcrop.2015.05.019
11. Sugiharto S, Tri Wibowo A, Zubaidah U, Dwi Savitri A, Faukib MS, Safira Salsabila N, et al. Biological Properties of Gynura procumbens Leaves Extract to MDA Levels and Antioxidant Activities in Liver of Mice. Res J Pharm Technol. 2022 Dec 24; 5829–34. DOI: 10.5958/0974-360X
12. Kaewseejan N, Sutthikhum V, Siriamornpun S. Potential of Gynura procumbens leaves as source of flavonoid-enriched fractions with enhanced antioxidant capacity. J Funct Foods. 2015; 12: 120–8. DOI: https://doi.org/10.1016/j.jff.2014.11.001
13. Geraldy A, Wardana AP, Aminah NS, Kristanti AN, Sadila AY, Wijaya NH, Wijaya MRA, Diningrum NID, Hajar VR, Manuhara YSW. Tropical medicinal plant extracts from Indonesia as antifungal agents against Candida albicans. Front. Biosci. (Landmark Ed). 2022; 27(9): 274. https://doi.org/10.31083/j.fbl2709274
14. Ferdinal N, Nazir N. Phytochemical screening and fractionation of gambier (Uncaria gambier). The Indonesian Society of Biomaterial Chemistry. 2013; 1(1): 23–6. DOI: 10.3390/molecules27196551
15. Andasuryani A, Purwanto YA, Budiastra IW, Syamsu K. Determination of Catechin Content in Gambir Powder from Dried Gambir Leaves Quickly using FT NIR PLS Model. Int J Adv Sci Eng Inf Technol. 2014; 4(5): 303. DOI: http://dx.doi.org/10.18517/ijaseit.4.5.423
16. Taniguchi S, Kuroda K, Doi K ichi, Tanabe M, Shibata T, Yoshida T, et al. Revised Structures of Gambiriins A1, A2, B1, and B2, Chalcane-Flavan Dimers from Gambir (Uncaria gambir Extract). Chem Pharm Bull (Tokyo). 2007; 55(2): 268–72. DOI: 10.1248/cpb.55.268
17. Nur Sazwi N, Nalina T, Rahim ZHA. Antioxidant and cytoprotective activities of Piper betle, Areca catechu, Uncaria gambir and betel quid with and without calcium hydroxide. BMC Complement Altern Med. 2013; Dec 11; 13(1): 351. DOI: 10.1186/1472-6882-13-351
18. Kasim A, Malrianti Y, Derosya V, Syukri D. GC-MS Screening of Valuable Volatile Compounds in the Waste of Uncaria gambir. Ann Biol. 2019;35(2):242–5.
19. Merlini L, Mondelli R, Nasini G, Hesse M. The structure of roxburghines A–E, new indole alkaloids from an Uncaria Sp. Tetrahedron. 1970 Jan; 26(10): 2259–79.
20. Gulcin İ, Alwasel SH. DPPH Radical Scavenging Assay. Processes. 2023 Jul 26; 11(8): 2248.
21. Scherer R, Godoy HT. Antioxidant activity index (AAI) by the 2,2-diphenyl-1-picrylhydrazyl method. Food Chem. 2009 Feb; 112(3): 654–8.
22. Jun M, Fu H ‐Y., Hong J, Wan X, Yang CS, Ho C ‐T. Comparison of Antioxidant Activities of Isoflavones from Kudzu Root (Pueraria lobata Ohwi). J Food Sci. 2003; Aug 20; 68(6): 2117–22. https://doi.org/10.1111/j.1365-2621.2003.tb07029.x
23. Askin H, Yilmaz B, Gulcin I, Taslimi P, Bakirci S, Yildiz M, et al. Antioxidant Activity of the Aqueous Extract of Iris taochia and Identification of its Chemical Constituents. Indian J Pharm Sci. 2018; 80(5). 10.4172/pharmaceutical-sciences.1000425
24. Rauf R, Santoso U, Suparmo. Aktivitas Penangkapan Radikal DPPH Ekstrak Gambir (Uncaria gambir Roxb.). Agritech. 2010; 30(1).
25. Halisa N, Frethernety A, Permatasari S, Alexandra FD, Ysrafil Y. Aktivitas Antioksidan Ekstrak Etanol Bajakah Kalalawit (Uncaria gambir (W.Hunter) Roxb) Menggunakan Metode DPPH. Jurnal Surya Medika. 2024 Aug 4; 10(2): 144–50.
26. Hidayati M, Rahmatulloh A. Antioxidant Activity of Uncaria gambir (Hunter) Roxb Extracts. Tropical Journal of Natural Product Research. 2022; 6(8): 1215–8.
27. Prior RL, Wu X, Schaich K. Standardized Methods for the Determination of Antioxidant Capacity and Phenolics in Foods and Dietary Supplements. J Agric Food Chem. 2005 May 1; 53(10): 4290–302. DOI: 10.1021/jf0502698.
28. Hartanti L, Ashari AM, Warsidah W. Total Phenol and Antioxidant Activity of Ethanol Extract and Water Extract from Claw Uncariaa gambir Roxb. Berkala Saintek. 2021 Aug 9; 9(3): 131. DOI: 10.19184/bst.v9i3.27179
29. Sindu Sakti A, Saputri FC, Munim A. Microscopic Characters, Phytochemical Screening Focus on Alkaloid and Total Phenolic Content of Uncaria gambir Roxb. and Uncaria sclerophylla Roxb. Leaves. Pharmacognosy Journal. 2019 Jan 7; 11(1): 119–23. DOI: 10.5530/pj.2019.1.20
30. Amir M, Mujeeb M, Khan A, Ashraf K, Sharma D, Aqil M. Phytochemical analysis and in vitro antioxidant activity of Uncaria gambir. International Journal of Green Pharmacy. 2012; 67–72. DOI: 10.4103/0973-8258.97136
31. Chang CC, Yang MH, Wen HM, Chern JC. Estimation of total flavonoid content in propolis by two complementary colometric methods. J Food Drug Anal. 2020 Jul 14; 10(3).
32. Ghanimi R, Ouhammou A, El Atki Y, El Hassan Bouchari M, Cherkaoui M. The Antioxidant Activities of Ethanolic, Methanolic, Ethyl Acetate, and Aqueous Extracts of the Endemic Species, Lavandula mairei Humbert (A Comparative Study between Cold and Hot Extraction). Ethiop J Health Sci. 2022; Nov; 32(6): 1231–6.
33. Amir M, Mujeeb M, Khan A, Ashraf K, Sharma D, Aqil M. Phytochemical analysis and in vitro antioxidant activity of Uncaria gambir. International Journal of Green Pharmacy. 2012; 6(1): 67–72.
34. Alfani NR, Febrianti R, Amananti W. Analysis of Total Flavonoid Content in the Extract of Bajakah Kalalawit Root (Uncaria gambir Roxb) Infunded Results. Indonesian Journal of Chemical Science and Technology (IJCST). 2023 Feb 1; 6(1): 65.
35. Gbashi S, Njobeh P, Steenkamp P, Tutu H, Madala N. The effect of temperature and methanol–water mixture on pressurized hot water extraction (PHWE) of anti-HIV analogoues from Bidens pilosa. Chem Cent J. 2016 Dec 8; 10(1): 37.
36. Gallina L, Cravotto C, Capaldi G, Grillo G, Cravotto G. Plant Extraction in Water: Towards Highly Efficient Industrial Applications. Processes. 2022 Oct 31; 10(11): 2233.
37. Rahman ED, Sari E, Burmawi, Frizka, Endah. Determination of Extraction Process Conditions of Gambier Catechin (Uncaria Gambier Roxb) from Solok Bio Bio Lima Puluh Kota District – West Sumatera. IOP Conf Ser Mater Sci Eng. 2018 Mar; 316: 012022.
38. Ibrahim N, Mohd Yusoff NZ, Ahmad R. Quantification of Catechin in Leaves and Stems of Malaysian Uncaria Gambir (Hunter) Roxb. By HPLC-DAD. Malaysian Journal of Analytical Science. 2016 Jun 15; 20(3): 567–72.
39. Getachew AT, Holdt SL, Meyer AS, Jacobsen C. Effect of Extraction Temperature on Pressurized Liquid Extraction of Bioactive Compounds from Fucus vesiculosus. Mar Drugs. 2022 Apr 12; 20(4).
40. Zubaidah U, Sugiharto, Siregar MIP, Islamatasya U, Nisa N, Wibowo AT, et al. Gynura procumbens Adventitious Root Ameliorates Oxidative Stress and has Cytotoxic Activity Against Cancer. BIO Integration. 2024; 5(1).
41. Sugiharto S, Faradila D, Rizqi Aningrum K, Nur Hakiki FD, Zahrotus Sa’adah A, Fatimatuz Zahroh U, et al. Potential of Jahe Merah (Zingiber officinale) and Temu Kunci (Boesenbergia rotunda): As Stimulator of Antioxidant Enzyme Activity and Anticancer in HepG2 Cell Line. Res J Pharm Technol. 2024 Aug 26; 3599–606.
42. GBD 2019 Antimicrobial Resistance Collaborators. Global mortality associated with 33 bacterial pathogens in 2019: a systematic analysis for the Global Burden of Disease Study 2019. Lancet. 2022 Dec 17; 400(10369): 2221–48.
43. Bhattacharya D, Bhattacharya S, Patra MM, Chakravorty S, Sarkar S, Chakraborty W, et al. Antibacterial Activity of Polyphenolic Fraction of Kombucha Against Enteric Bacterial Pathogens. Curr Microbiol. 2016 Dec; 73(6): 885–96.
44. Abdul Qadir M, Shahzadi SK, Bashir A, Munir A, Shahzad S. Evaluation of Phenolic Compounds and Antioxidant and Antimicrobial Activities of Some Common Herbs. Int J Anal Chem. 2017; 2017: 3475738.